Pesticidal Genes and Methods of Use

ABSTRACT

Compositions having pesticidal activity and methods for their use are provided. Compositions include isolated and recombinant polypeptides having pesticidal activity, recombinant and synthetic nucleic acid molecules encoding the polypeptides, DNA constructs and vectors comprising the nucleic acid molecules, host cells comprising the vectors, and antibodies to the polypeptides. Nucleotide sequences encoding the polypeptides can be used in DNA constructs or expression cassettes for transformation and expression in organisms of interest. The compositions and methods provided are useful for producing organisms with enhanced pest resistance or tolerance. Transgenic plants and seeds comprising a nucleotide sequence that encodes a pesticidal protein of the invention are also provided. Such plants are resistant to insects and other pests. Methods are provided for producing the various polypeptides disclosed herein, and for using those polypeptides for controlling or killing a pest. Methods and kits for detecting polypeptides of the invention in a sample are also included.

CROSS REFERENCE TO RELATED APPLICATION

This application is a divisional application of U.S. application Ser.No. 16/184,769, filed Nov. 8, 2018, which is a divisional application ofU.S. application Ser. No. 15/702,343, filed Sep. 12, 2017, now U.S. Pat.No. 10,167,324, issued Jan. 1, 2019, which is a divisional applicationof U.S. application Ser. No. 15/099,919, filed Apr. 15, 2016, now U.S.Pat. No. 10,053,707, issued Aug. 21, 2018, which claims the benefit ofU.S. Provisional Application Ser. No. 62/151,156, filed Apr. 22, 2015,the contents of the applications are herein incorporated by reference inits entirety.

FIELD

The invention is drawn to methods and compositions for controllingpests, particularly plant pests.

REFERENCE TO A SEQUENCE LISTING SUBMITTED AS A TEXT FILE VIA EFS-WEB

The official copy of the sequence listing is submitted electronicallyvia EFS-Web as an ASCII formatted sequence listing with a file namedAgB012USDIV3_seqlisting.txt, created on Oct. 9, 2019, and having a sizeof 1.5 MB and is filed concurrently with the specification. The sequencelisting contained in this ASCII formatted document is part of thespecification and is herein incorporated by reference in its entirety.

BACKGROUND

Pests, plant diseases, and weeds can be serious threats to crops. Lossesdue to pests and diseases have been estimated at 37% of the agriculturalproduction worldwide, with 13% due to insects, bacteria and otherorganisms.

Toxins are virulence determinants that play an important role inmicrobial pathogenicity and/or evasion of the host immune response.Toxins from the gram-positive bacterium Bacillus, particularly Bacillusthuringiensis, have been used as insecticidal proteins. Currentstrategies use the genes expressing these toxins to produce transgeniccrops. Transgenic crops expressing insecticidal protein toxins are usedto combat crop damage from insects.

While the use of Bacillus toxins has been successful in controllinginsects, resistance to Bt toxins has developed in some target pests inmany parts of the world where such toxins have been used intensively.One way of solving this problem is sowing Bt crops with alternating rowsof regular non Bt crops (refuge). An alternative method to avoid or slowdown development of insect resistance is stacking insecticidal geneswith different modes of action against insects in transgenic plants. Thecurrent strategy of using transgenic crops expressing insecticidalprotein toxins is placing increasing emphasis on the discovery of noveltoxins, beyond those already derived from the bacterium Bacillusthuringiensis. These toxins may prove useful as alternatives to thosederived from B. thuringiensis for deployment in insect- andpest-resistant transgenic plants. Thus, new toxin proteins are needed.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 provides an alignment of SEQ ID NOS: 177, 307, 372, 374, and 396.Divergent amino acids are highlighted in grey.

FIG. 2 provides the global sequence identity relationship between SEQ IDNOS: 177, 307, 372, 374, and 396.

SUMMARY

Compositions having pesticidal activity and methods for their use areprovided. Compositions include isolated and recombinant polypeptidesequences having pesticidal activity, recombinant and synthetic nucleicacid molecules encoding the pesticidal polypeptides, DNA constructscomprising the nucleic acid molecules, vectors comprising the nucleicacid molecules, host cells comprising the vectors, and antibodies to thepesticidal polypeptides. Nucleotide sequences encoding the polypeptidesprovided herein can be used in DNA constructs or expression cassettesfor transformation and expression in organisms of interest, includingmicroorganisms and plants.

The compositions and methods provided herein are useful for theproduction of organisms with enhanced pest resistance or tolerance.These organisms and compositions comprising the organisms are desirablefor agricultural purposes. Transgenic plants and seeds comprising anucleotide sequence that encodes a pesticidal protein of the inventionare also provided. Such plants are resistant to insects and other pests.

Methods are provided for producing the various polypeptides disclosedherein, and for using those polypeptides for controlling or killing apest. Methods and kits for detecting polypeptides of the invention in asample are also included.

DETAILED DESCRIPTION

The present inventions now will be described more fully hereinafter withreference to the accompanying drawings, in which some, but not allembodiments of the is inventions are shown. Indeed, these inventions maybe embodied in many different forms and should not be construed aslimited to the embodiments set forth herein; rather, these embodimentsare provided so that this disclosure will satisfy applicable legalrequirements. Like numbers refer to like elements throughout.

Many modifications and other embodiments of the inventions set forthherein will come to mind to one skilled in the art to which theseinventions pertain having the benefit of the teachings presented in theforegoing descriptions and the associated drawings. Therefore, it is tobe understood that the inventions are not to be limited to the specificembodiments disclosed and that modifications and other embodiments areintended to be included within the scope of the appended claims.Although specific terms are employed herein, they are used in a genericand descriptive sense only and not for purposes of limitation.

I. Polynucleotides and Polypeptides

Compositions and method for conferring pesticidal activity to anorganism are provided. The modified organism exhibits pesticidalresistance or tolerance.

Recombinant pesticidal proteins, or polypeptides and fragments andvariants thereof that retain pesticidal activity, are provided andinclude those set forth in SEQ ID NOs: 1-398. The pesticidal proteinsare biologically active (e.g., pesticidal) against pests includinginsects, fungi, nematodes, and the like. Nucleotides encoding thepesticidal polypeptides, including for example, SEQ ID NOS: 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23,24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41,42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59,60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77,78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138,139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152,153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180,181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194,195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208,209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222,223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236,237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250,251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264,265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278,279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320,321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334,335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362,363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376,377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390,391, 392, 393, 394, 395, 396, 397, or 398 or active fragments orvariants thereof, can be used to produce transgenic organisms, such asplants and microorganisms. The pesticidal proteins are biologicallyactive (for example, are pesticidal) against pests including insects,fungi, nematodes, and the like. Polynucleotides encoding the pesticidalpolypeptides, including for example, SEQ ID NOS: 1-398 or activefragments or variants thereof, can be used to produce transgenicorganisms, such as plants and microorganisms. The transformed organismsare characterized by genomes that comprise at least one stablyincorporated DNA construct comprising a coding sequence for a pesticidalprotein disclosed herein. In some embodiments, the coding sequence isoperably linked to a promoter that drives expression of the encodedpesticidal polypeptide. Accordingly, transformed microorganisms, plantcells, plant tissues, plants, seeds, and plant parts are provided. Asummary of various polypeptides, active variants and fragments thereof,and polynucleotides encoding the same are set forth below in Table 1. Asnoted in Table 1, various forms of polypeptides are provided. Fulllength pesticidal polypeptides, as well as, modified versions of theoriginal full-length sequence (i.e., variants) are provided. Table 1further denotes “CryBP1” sequences. Such sequences comprise accessorypolypeptides that can be associated with some of the toxin genes. Insuch instances, the CryBP1 sequences can be used alone or in combinationwith any of the pesticidal polypeptides provided herein. Table 1 furtherprovides Split-Cry C-terminus polypeptides. Such sequences comprise thesequence of a downstream protein that has is homology to the C-terminalend of the Cry class of toxin genes and are usually found after a Crygene that is not full-length and is missing the expected C-terminalregion.

TABLE 1 Summary of SEQ ID NOs, Gene Class, and Variants thereofPolypeptides of the invention Polypeptides of the invention Split-Cry(and polynucleotides (and polynucleotides Full- Modified CryBP1C-terminus encoding the same) include encoding the same) include Genelength SEQID SEQ ID SEQ ID Gene those having the % sequence those havingthe similarity set Name SEQ ID No. No.(s) No. No. Homologs Classidentity listed below forth below APG00056 1 2 AGU13855.1 (69.6%identity, 79.2% similarity) Cry32 70, 75, 80, 85, 90, 95, 96, 97, 80,85, 90, 95, 96, 97, 98, 99 98, 99 AGU13869.1 (68.4% identity, 79.1%similarity) US20110203014_23 (68.4% identity, 79.1% similarity) APG00058(63.6% identity, 75.5% similarity) Cry32Ea1 (55.1% identity, 65.9%similarity) APG00068 (52.0% identity, 64.2% similarity) APG00105 (50.5%identity, 61.8% similarity) APG00054 (50.3% identity, 60.9% similarity)APG00058 3 4 AGU13869.1 (74.6% identity, 81.5% similarity) Cry32 75, 80,85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98, 99 99 US20110203014_23(74.6% identity, 81.5% similarity) AGU13855.1 (69.3% identity, 77.8%similarity) APG00056 (63.6% identity, 75.5% similarity) Cry32Da1 (52.7%identity, 64.1% similarity) APG00064 5 6 US20130227743A1_58 (66.4%identity, 78.7% similarity) Mtx 70, 75, 80, 85, 90, 95, 96, 97, 80, 85,90, 95, 96, 97, 98, 99 98, 99 WP_000875422.1 (65.8% identity, 79.6%similarity) US20060191034A1_10 (65.8% identity, 78.7% similarity)WP_000875423.1 (65.2% identity, 79.3% similarity) APG00055 (64.2%identity, 78.5% similarity) APG00174 (61.6% identity, 74.9% similarity)APG00768 (57.9% identity, 73.5% similarity) APG00067 7 APG00408 (81.6%identity, 89.3% similarity) Mtx 75, 80, 85, 90, 95, 96, 97, 98, 85, 90,95, 96, 97, 98, 99 99 APG00559 (75.5% identity, 81.2% similarity)APG00006 (74.4% identity, 85.9% similarity) APG00201 (74.4% identity,84.9% similarity) WP_000963933.1 (72.6% identity, 84.3% similarity)US20130227743A1_100 (72.5% identity, 83.2% similarity) APG00155 (71.8%identity, 81.5% similarity) APG00107 (71.5% identity, 83.6% similarity)APG00022 (71.1% identity, 83.7% similarity) APG00036 (70.9% identity,80.5% similarity) US20130227743A1_60 (40.7% identity, 46.9% similarity)WP_026324166.1 (26.9% identity, 42.7% similarity) APG00071 8 9 APG00150(95.9% identity, 98.6% similarity) Vip 25, 30, 35, 40, 45, 50, 55, 60,35, 40, 45, 50, 55, 60, 65, 70, 65, 70, 75, 80, 85, 90, 95, 96, 75, 80,85, 90, 95, 96, 97, 98, 97, 98, 99 99 CAI96522.1 (22.2% identity, 34.9%similarity) US_6369213_B1-94 (22.2% identity, 34.9% similarity)AFJ19238.1 (22.2% identity, 34.8% similarity) Vip3Aa49 (22.2% identity,34.7% similarity) APG00073 10 11, 12, 13 CR5AC_BACTU (64.9% identity,71.0% similarity) Cry5 65, 70, 75, 80, 85, 90, 95, 96, 75, 80, 85, 90,95, 96, 97, 98, 97, 98, 99 99 US_5281530-3 (64.4% identity, 71.9%similarity) Cry5Ab1 (64.4% identity, 71.8% similarity) APG00074 14 228KEZ80024.1 (39.4% identity, 48.8% similarity) Vip 40, 45, 50, 55, 60,65, 70, 75, 50, 55, 60, 65, 70, 75, 80, 85, 80, 85, 90, 95, 96, 97, 98,99 90, 95, 96, 97, 98, 99 Vip3Ag2 (23.6% identity, 40.2% similarity)AIT93180.1 (23.6% identity, 40.1% similarity) APG00084 15  16, 229APG00152 (59.4% identity, 69.8% similarity) Cry 60, 65, 70, 75, 80, 85,90, 95, 75, 80, 85, 90, 95, 96, 97, 98, 96, 97, 98, 99 99US20130227743A1_12 (57.6% identity, 70.5% similarity) WP_003308604.1(55.4% identity, 66.8% similarity) APG00164 (53.6% identity, 68.1%similarity) CBL59394.1 (46.2% identity, 63.8% similarity) Cry8Aa1 (28.8%identity, 38.0% similarity) APG00105 17 18, 19, 20 AGU13822.1 (78.4%identity, 84.5% similarity) Cry32 80, 85, 90, 95, 96, 97, 98, 99 85, 90,95, 96, 97, 98, 99 AGU13821.1 (57.0% identity, 69.8% similarity)AGU13871.1 (56.1% identity, 68.2% similarity) Cry32Ab1 (54.2% identity,66.7% similarity) APG00054 (53.4% identity, 65.2% similarity) APG00068(52.1% identity, 65.4% similarity) APG00056 (50.5% identity, 61.8%similarity) APG00107 21 APG00155 (78.0% identity, 87.5% similarity) Mtx80, 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99 WP_000963933.1(77.4% identity, 88.9% similarity) US20130227743A1_100 (77.0% identity,87.8% similarity) APG00201 (75.3% identity, 83.6% similarity) APG00006(74.9% identity, 84.4% similarity) APG00067 (71.5% identity, 83.6%similarity) APG00036 (71.3% identity, 80.0% similarity) APG00022 (69.8%identity, 80.4% similarity) APG00137 (69.4% identity, 81.2% similarity)APG00898 (64.8% identity, 78.8% similarity) US20130227743A1_60 (39.0%identity, 44.9% similarity) WP_012181803.1 (23.0% identity, 37.8%similarity) APG00108 22 230 WP_030313032.1 (71.8% identity, 72.2%similarity) Cyt 75, 80, 85, 90, 95, 96, 97, 98, 75, 80, 85, 90, 95, 96,97, 98, 99 99 WP_015038174.1 (69.5% identity, 71.1% similarity) APG00134(68.8% identity, 69.9% similarity) WP_030212110.1 (66.9% identity, 69.5%similarity) APG00198 (62.0% identity, 66.5% similarity) Cyt2Ca1 (19.9%identity, 34.7% similarity) APG00112 23 AGA40062.1 (80.6% identity,88.3% similarity) Mtx 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99AGA40061.1 (61.3% identity, 73.9% similarity) APG00907 (57.7% identity,69.5% similarity) Cry60Ba1 (36.2% identity, 51.6% similarity) APG0011324 231, 232 APG00196 (91.0% identity, 95.3% similarity) Cry 70, 75, 80,85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99 APG00030 (71.4%identity, 84.0% similarity) AGP17989.1 (68.7% identity, 82.6%similarity) APG00096 (68.1% identity, 82.2% similarity) APG00114 (58.5%identity, 77.2% similarity) AGP17990.1 (57.9% identity, 68.4%similarity) APG00500 (56.6% identity, 65.3% similarity)US_2013_0227743_A1_194 (41.3% identity, 47.4% similarity) Cry42Aa1(23.6% identity, 37.1% similarity) APG00116 25  26, 233, 234 AGP18043.1(94.2% identity, 96.6% similarity) Cry 95, 96, 97, 98, 99 97, 98, 99AFM37573.1 (93.9% identity, 96.1% similarity) AGV55020.1 (86.8%identity, 92.6% similarity) APG00153 (53.8% identity, 64.4% similarity)Cry53Aa1 (39.8% identity, 54.8% similarity) APG00117 27 235, 236APG00186 (97.2% identity, 97.4% similarity) Cyt 35, 40, 45, 50, 55, 60,65, 70, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96, 97, 98,75, 80, 85, 90, 95, 96, 97, 98, 99 99 AJF68767.1 (31.8% identity, 32.3%similarity) WP_030689537.1 (31.5% identity, 32.2% similarity)WP_030313032.1 (30.4% identity, 32.0% similarity) Cyt2Aa2 (10.5%identity, 18.6% similarity) APG00118 28 237 APG00223 (91.0% identity,95.2% similarity) Bin 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99APG00913 (90.9% identity, 92.7% similarity) APG00454 (90.3% identity,94.9% similarity) APG00242 (90.3% identity, 94.7% similarity)WP_002187944.1 (84.6% identity, 86.9% similarity) US20130227743A1_10(81.8% identity, 86.2% similarity) WP_001258160.1 (81.6% identity, 85.9%similarity) APG00065 (58.1% identity, 70.6% similarity) Cry35Ba1 (18.8%identity, 32.5% similarity) APG00121 29 238 APG00177 (68.6% identity,79.9% similarity) Cyt 55, 60, 65, 70, 75, 80, 85, 90, 70, 75, 80, 85,90, 95, 96, 97, 95, 96, 97, 98, 99 98, 99 APG00126 (54.7% identity,68.1% similarity) WP_016110459.1 (54.3% identity, 63.7% similarity)APG00128 (54.3% identity, 63.7% similarity) WP_016110460.1 (51.8%identity, 66.4% similarity) CT2BB_BACTJ (38.9% identity, 52.5%similarity) Cyt2Ca1 (37.6% identity, 50.9% similarity) APG00131 30Vip3Ag5 (29.9% identity, 44.1% similarity) Vip 30, 35, 40, 45, 50, 55,60, 65, 45, 50, 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 95, 96, 97,85, 90, 95, 96, 97, 98, 99 98, 99 APG00132 31 239 APG00249 (88.9%identity, 94.7% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90,95, 96, 97, 98, 99 99 APG00459 (88.0% identity, 94.1% similarity)APG00065 (77.7% identity, 85.0% similarity) APG00407 (75.9% identity,85.2% similarity) APG00229 (73.7% identity, 82.4% similarity)WP_000839920.1 (71.9% identity, 82.9% similarity) WP_002166959.1 (68.4%identity, 79.7% similarity) US20130227743A1_146 (68.2% identity, 79.4%similarity) Cry35Ab3 (20.7% identity, 35.2% similarity) APG00134 32 240WP_030313032.1 (94.8% identity, 96.9% similarity) Cyt 95, 96, 97, 98, 9997, 98, 99 WP_015038174.1 (93.8% identity, 96.4% similarity)WP_030212110.1 (93.2% identity, 96.4% similarity) APG00198 (86.5%identity, 92.2% similarity) APG00108 (68.8% identity, 69.9% similarity)Cyt2Ca1 (24.2% identity, 42.0% similarity) APG00137 33 241 APG00006(71.8% identity, 83.6% similarity) Mtx 75, 80, 85, 90, 95, 96, 97, 98,85, 90, 95, 96, 97, 98, 99 99 APG00201 (71.7% identity, 81.2%similarity) WP_000963933.1 (71.6% identity, 82.5% similarity)US20130227743A1_100 (70.0% identity, 81.5% similarity) APG00107 (69.4%identity, 81.2% similarity) APG00898 (69.2% identity, 80.0% similarity)APG00067 (67.2% identity, 81.6% similarity) APG00036 (67.2% identity,78.0% similarity) APG00408 (66.2% identity, 78.0% similarity) APG00022(65.2% identity, 77.0% similarity) US20130227743A1_60 (39.2% identity,46.7% similarity) WP_012181803.1 (24.5% identity, 36.9% similarity)APG00141 34 242 WP_000727408.1 (93.5% identity, 96.6% similarity) Bin95, 96, 97, 98, 99 97, 98, 99 WP_016110923.1 (93.2% identity, 96.3%similarity) WP_000727409.1 (92.7% identity, 95.8% similarity) APG00237(61.2% identity, 71.8% similarity) APG00261 (60.5% identity, 71.5%similarity) APG00913 (54.0% identity, 65.1% similarity) APG00065 (53.4%identity, 67.1% similarity) APG00398 (50.8% identity, 60.9% similarity)Cry35Ab5 (22.2% identity, 38.4% similarity) APG00150 35 243 APG00071(95.9% identity, 98.6% similarity) Vip 25, 30, 35, 40, 45, 50, 55, 60,35, 40, 45, 50, 55, 60, 65, 70, 65, 70, 75, 80, 85, 90, 95, 96, 75, 80,85, 90, 95, 96, 97, 98, 97, 98, 99 99 Vip3Aa49 (22.4% identity, 34.8%similarity) APG00152 36 37 US20130227743A1_12 (69.7% identity, 80.8%similarity) Cry 70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98,99 98, 99 WP_003308604.1 (66.0% identity, 75.9% similarity) APG00084(59.4% identity, 69.8% similarity) D5H3I8_BACTG (45.3% identity, 62.9%similarity) Cry8Aa1 (27.8% identity, 37.8% similarity) APG00153 38  39,244, 245 AGP18043.1 (54.1% identity, 65.0% similarity) Cry 55, 60, 65,70, 75, 80, 85, 90, 70, 75, 80, 85, 90, 95, 96, 97, 95, 96, 97, 98, 9998, 99 AFM37573.1 (53.9% identity, 64.7% similarity) APG00116 (53.8%identity, 64.4% similarity) AGV55020.1 (53.4% identity, 65.3%similarity) Cry8Ba1 (21.6% identity, 33.6% similarity) APG00155 40WP_000963933.1 (78.5% identity, 85.9% similarity) Mtx 80, 85, 90, 95,96, 97, 98, 99 90, 95, 96, 97, 98, 99 APG00107 (78.0% identity, 87.5%similarity) US20130227743A1_100 (77.0% identity, 85.1% similarity)APG00201 (76.3% identity, 83.9% similarity) APG00006 (75.7% identity,83.0% similarity) APG00036 (74.8% identity, 82.4% similarity) APG00022(72.1% identity, 80.7% similarity) APG00067 (71.8% identity, 81.5%similarity) APG00898 (67.1% identity, 79.3% similarity) APG00137 (66.4%identity, 77.6% similarity) US20130227743A1_60 (41.6% identity, 46.9%similarity) WP_000794513.1 (22.9% identity, 35.3% similarity) APG0016441 42 CBL59393.1 (58.2% identity, 73.3% similarity) Cry 60, 65, 70, 75,80, 85, 90, 95, 75, 80, 85, 90, 95, 96, 97, 98, 96, 97, 98, 99 99D5H3J0_BACTG (58.2% identity, 73.3% similarity) D5H3I8_BACTG (58.0%identity, 73.2% similarity) APG00084 (53.6% identity, 68.1% similarity)Cry8Aa1 (37.2% identity, 43.6% similarity) APG00166 43 246 KEZ80024.1(34.1% identity, 37.7% similarity) Vip 35, 40, 45, 50, 55, 60, 65, 70,40, 45, 50, 55, 60, 65, 70, 75, 75, 80, 85, 90, 95, 96, 97, 98, 80, 85,90, 95, 96, 97, 98, 99 99 US_7129212_B2-19 (17.5% identity, 28.4%similarity) US_7129212_B2-17 (17.1% identity, 28.4% similarity) Vip3Ag2(16.8% identity, 27.9% similarity) APG00168 44 247 APG00015 (60.3%identity, 71.4% similarity) Cyt2 60, 65, 70, 75, 80, 85, 90, 95, 75, 80,85, 90, 95, 96, 97, 98, 96, 97, 98, 99 99 Cyt2Aa2 (59.5% identity, 71.8%similarity) APG00172 45 AHN21707.1 (72.8% identity, 84.3% similarity)Mtx 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98, 99 99WP_024361905.1 (72.8% identity, 84.3% similarity) WP_020721402.1 (58.1%identity, 74.8% similarity) Cry46Ab (37.2% identity, 52.6% similarity)APG00173 46 47 APG00278 (94.5% identity, 96.8% similarity) Vip 75, 80,85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98, 99 99 APG00077 (82.4%identity, 87.7% similarity) APG00175 (80.5% identity, 86.0% similarity)KEZ80024.1 (73.6% identity, 84.3% similarity) APG00273 (68.8% identity,78.1% similarity) APG00104 (68.7% identity, 78.1% similarity) APG00033(68.1% identity, 77.4% similarity) Vip3Aa13 (24.3% identity, 41.6%similarity) APG00175 48 49 APG00077 (97.4% identity, 97.9% similarity)Vip 70, 75, 80, 85, 90, 95, 96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98,99 APG00278 (80.7% identity, 86.6% similarity) APG00173 (80.5% identity,86.0% similarity) APG00273 (78.0% identity, 85.7% similarity) APG00033(76.9% identity, 84.7% similarity) APG00104 (71.3% identity, 80.3%similarity) KEZ80024.1 (68.1% identity, 78.7% similarity) Vip3Ai1 (25.0%identity, 42.7% similarity) APG00176 50 51 AGP18009.1 (44.3% identity,58.8% similarity) Cry 45, 50, 55, 60, 65, 70, 75, 80, 60, 65, 70, 75,80, 85, 90, 95, 85, 90, 95, 96, 97, 98, 99 96, 97, 98, 99 AGP17994.1(44.2% identity, 58.3% similarity) US_8461415_B2-49 (42.2% identity,56.1% similarity) Cry53Aa1 (34.7% identity, 49.1% similarity) APG0017752  53, 248 APG00121 (68.6% identity, 79.9% similarity) Cyt 60, 65, 70,75, 80, 85, 90, 95, 75, 80, 85, 90, 95, 96, 97, 98, 96, 97, 98, 99 99WP_016110459.1 (56.8% identity, 68.9% similarity) APG00128 (56.6%identity, 69.9% similarity) APG00126 (56.5% identity, 73.1% similarity)WP_016110460.1 (55.4% identity, 72.5% similarity) US_6686452-2 (42.1%identity, 54.5% similarity) Cyt2Ca1 (41.7% identity, 54.1% similarity)APG00178 54 APG00727 (90.7% identity, 93.6% similarity) Bin 30, 35, 40,45, 50, 55, 60, 65, 45, 50, 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90,95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99 APG00315 (87.7% identity,91.6% similarity) Cry49Ab1 (27.7% identity, 44.2% similarity) APG0018055 WP_000240776.1 (79.3% identity, 91.2% similarity) Cry6 80, 85, 90,95, 96, 97, 98, 99 95, 96, 97, 98, 99 WP_000240775.1 (79.0% identity,90.7% similarity) WP_016078427.1 (79.0% identity, 90.4% similarity)APG00024 (77.7% identity, 88.9% similarity) APG00342 (62.9% identity,78.8% similarity) Cry6Ba1 (27.2% identity, 48.1% similarity) APG00186 56249, 250 APG00117 (97.2% identity, 97.4% similarity) Cyt 35, 40, 45, 50,55, 60, 65, 70, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 96,97, 98, 75, 80, 85, 90, 95, 96, 97, 98, 99 99 AJF68767.1 (31.8%identity, 32.3% similarity) WP_030689537.1 (31.5% identity, 32.2%similarity) WP_030313032.1 (30.4% identity, 32.0% similarity) Cyt2Aa2(10.6% identity, 18.8% similarity) APG00188 57 58 APG00007 (76.5%identity, 83.7% similarity) Cry 75, 80, 85, 90, 95, 96, 97, 98, 85, 90,95, 96, 97, 98, 99 99 AGP18022.1 (74.9% identity, 83.0% similarity)AEH76820.1 (44.4% identity, 59.2% similarity) US20130227743A1_48 (44.3%identity, 58.5% similarity) Cry32Ca1 (44.3% identity, 57.3% similarity)APG00189 59 60, 61 APG00087 (90.3% identity, 94.0% similarity) Cry 30,35, 40, 45, 50, 55, 60, 65, 45, 50, 55, 60, 65, 70, 75, 80, 70, 75, 80,85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99 US_8759619_B2-30(28.0% identity, 43.2% similarity) US_7253343_B2-17 (27.7% identity,41.1% similarity) Cry1Ib4 (27.3% identity, 40.9% similarity) APG00190 6263 WP_001083588.1 (69.3% identity, 79.0% similarity) Cry26 70, 75, 80,85, 90, 95, 96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98, 99 AGT50919.1(68.1% identity, 78.0% similarity) Cry26Aa1 (65.4% identity, 75.3%similarity) APG00192 64 WP_002167240.1 (32.2% identity, 51.3%similarity) Bin 35, 40, 45, 50, 55, 60, 65, 70, 55, 60, 65, 70, 75, 80,85, 90, 75, 80, 85, 90, 95, 96, 97, 98, 95, 96, 97, 98, 99 99WP_002016877.1 (32.2% identity, 49.6% similarity) WP_016097060.1 (31.3%identity, 49.0% similarity) Cry35Ab2 (23.3% identity, 38.3% similarity)APG00194 65 251 APG00017 (57.4% identity, 72.2% similarity) Cyt 40, 45,50, 55, 60, 65, 70, 75, 55, 60, 65, 70, 75, 80, 85, 90, 80, 85, 90, 95,96, 97, 98, 99 95, 96, 97, 98, 99 US_8513493_B2-47 (35.7% identity,53.1% similarity) 2RCI_A (27.0% identity, 43.8% similarity) CT2BB_BACTJ(27.0% identity, 40.9% similarity) Cyt2Aa2 (26.9% identity, 42.8%similarity) APG00196 66 252 APG00113 (91.0% identity, 95.3% similarity)Cry 70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99APG00030 (69.9% identity, 82.5% similarity) AGP17989.1 (66.9% identity,81.1% similarity) APG00096 (66.6% identity, 80.9% similarity) APG00114(57.1% identity, 76.0% similarity) AGP17990.1 (56.6% identity, 67.0%similarity) APG00500 (51.7% identity, 62.1% similarity)US_2013_0227743_A1_194 (39.6% identity, 46.0% similarity) Cry42Aa1(23.4% identity, 36.7% similarity) APG00198 67 253 WP_024758398.1 (91.8%identity, 95.9% similarity) Cyt 95, 96, 97, 98, 99 96, 97, 98, 99WP_030208963.1 (90.7% identity, 94.3% similarity) WP_030545473.1 (89.8%identity, 92.9% similarity) APG00134 (86.5% identity, 92.2% similarity)APG00108 (62.0% identity, 66.5% similarity) Cyt2Ca1 (22.9% identity,40.3% similarity) APG00200 68 254, 255 APG00186 (53.9% identity, 58.7%similarity) Cyt 40, 45, 50, 55, 60, 65, 70, 75, 40, 45, 50, 55, 60, 65,70, 75, 80, 85, 90, 95, 96, 97, 98, 99 80, 85, 90, 95, 96, 97, 98, 99APG00117 (52.6% identity, 57.5% similarity) WP_030752998.1 (35.8%identity, 37.7% similarity) WP_030497750.1 (35.2% identity, 37.1%similarity) WP_031015242.1 (35.0% identity, 37.3% similarity) Cyt2Aa2(11.7% identity, 21.5% similarity) APG00203 69 256 APG00353 (95.3%identity, 96.6% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90,95, 96, 97, 98, 99 99 APG00301 (90.9% identity, 93.7% similarity)APG00844 (89.8% identity, 93.7% similarity) APG00412 (87.5% identity,91.3% similarity) APG00065 (75.7% identity, 83.2% similarity)WP_000839920.1 (73.0% identity, 82.8% similarity) WP_002166959.1 (70.4%identity, 80.9% similarity) WP_002191947.1 (70.2% identity, 80.9%similarity) Cry35Ad2 (22.9% identity, 36.8% similarity) APG00207 70Vip3Aa8 (37.8% identity, 53.6% similarity) Vip 40, 45, 50, 55, 60, 65,70, 75, 55, 60, 65, 70, 75, 80, 85, 90, 80, 85, 90, 95, 96, 97, 98, 9995, 96, 97, 98, 99 APG00209 71 257 WP_002128069.1 (73.1% identity, 85.5%similarity) Cry6 75, 80, 85, 90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 9999 WP_000591974.1 (73.1% identity, 85.2% similarity) WP_002185458.1(73.1% identity, 85.2% similarity) WP_002191530.1 (73.1% identity, 85.2%similarity) APG00210 72 258 WP_028939439.1 (59.6% identity, 74.1%similarity) Cry6 60, 65, 70, 75, 80, 85, 90, 95, 75, 80, 85, 90, 95, 96,97, 98, 96, 97, 98, 99 99 WP_015096649.1 (42.6% identity, 63.0%similarity) WP_019823923.1 (32.2% identity, 51.3% similarity)WP_016779464.1 (32.0% identity, 51.8% similarity) APG00211 73 74APG00368 (98.7% identity, 99.2% similarity) Bin 80, 85, 90, 95, 96, 97,98, 99 90, 95, 96, 97, 98, 99 APG00011 (96.2% identity, 97.3%similarity) APG00377 (79.1% identity, 87.4% similarity) APG00356 (79.1%identity, 86.9% similarity) APG00231 (78.6% identity, 86.9% similarity)APG00035 (76.9% identity, 85.5% similarity) WP_000143307.1 (76.4%identity, 85.8% similarity) WP_000143308.1 (75.1% identity, 85.3%similarity) US20130227743A1_6 (69.4% identity, 79.4% similarity)Cry35Ab4 (22.7% identity, 38.5% similarity) APG00212 75 259, 260APG00798 (92.7% identity, 96.8% similarity) Bin 80, 85, 90, 95, 96, 97,98, 99 85, 90, 95, 96, 97, 98, 99 APG00592 (89.8% identity, 94.2%similarity) APG00600 (87.1% identity, 92.0% similarity) APG00619 (86.2%identity, 92.0% similarity) US20130227743A1_146 (76.9% identity, 83.3%similarity) APG00065 (72.0% identity, 80.7% similarity) WP_002191947.1(71.2% identity, 79.2% similarity) WP_002166959.1 (70.9% identity, 79.2%similarity) Cry49Aa1 (22.4% identity, 34.4% similarity) APG00213 76 261APG00243 (82.7% identity, 89.1% similarity) Bin 75, 80, 85, 90, 95, 96,97, 98, 85, 90, 95, 96, 97, 98, 99 99 APG00844 (81.4% identity, 89.9%similarity) APG00203 (81.2% identity, 90.2% similarity) APG00353 (80.4%identity, 89.4% similarity) APG00065 (79.2% identity, 84.1% similarity)WP_000839920.1 (72.3% identity, 83.0% similarity) WP_002166959.1 (71.1%identity, 81.2% similarity) WP_002191947.1 (70.8% identity, 81.2%similarity) Cry35Ad2 (21.5% identity, 37.1% similarity) APG00214 77 262WP_033669474.1 (90.4% identity, 95.6% similarity) Cry6 95, 96, 97, 98,99 96, 97, 98, 99 EOP91866.1 (84.6% identity, 89.6% similarity)KFN04132.1 (73.4% identity, 86.0% similarity) WP_016092834.1 (72.8%identity, 84.6% similarity) APG00215 78 WP_016093722.1 (73.0% identity,82.5% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96,97, 98, 99 99 WP_002167240.1 (59.6% identity, 72.3% similarity) APG00386(59.0% identity, 73.7% similarity) WP_002090518.1 (53.4% identity, 70.7%similarity) Cry35Ad2 (22.8% identity, 40.5% similarity) APG00216 79APG00090 (73.0% identity, 73.3% similarity) Bin 55, 60, 65, 70, 75, 80,85, 90, 70, 75, 80, 85, 90, 95, 96, 97, 95, 96, 97, 98, 99 98, 99US20130227743A1_154 (54.1% identity, 69.7% similarity)US20130227743A1_156 (54.1% identity, 66.3% similarity) KEZ80012.1 (44.3%identity, 55.4% similarity) Cry49Ab1 (17.6% identity, 27.5% similarity)APG00219 80 263 AGA40057.1 (33.9% identity, 51.3% similarity) Cry 35,40, 45, 50, 55, 60, 65, 70, 55, 60, 65, 70, 75, 80, 85, 90, 75, 80, 85,90, 95, 96, 97, 98, 95, 96, 97, 98, 99 99 AGA40058.1 (29.9% identity,46.9% similarity) WP_017762619.1 (29.7% identity, 43.3% similarity)WP_017762581.1 (29.5% identity, 44.7% similarity) APG00221 81 264APG00376 (92.5% identity, 95.1% similarity) Bin 70, 75, 80, 85, 90, 95,96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98, 99 APG00237 (88.1% identity,91.6% similarity) APG00353 (77.0% identity, 84.8% similarity) APG00844(75.7% identity, 83.7% similarity) WP_002166959.1 (67.7% identity, 79.1%similarity) WP_000839920.1 (67.4% identity, 79.5% similarity)WP_002191947.1 (67.4% identity, 79.1% similarity) APG00065 (63.5%identity, 75.0% similarity) Cry35Ac2 (21.9% identity, 39.4% similarity)APG00223 82 265, 266 APG00242 (98.4% identity, 98.6% similarity) Bin 90,95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99 APG00454 (97.7% identity,98.4% similarity) APG00118 (91.0% identity, 95.2% similarity)US20130227743A1_10 (85.6% identity, 88.7% similarity) WP_001258160.1(85.4% identity, 88.4% similarity) WP_001258161.1 (85.2% identity, 88.2%similarity) APG00913 (85.1% identity, 90.0% similarity) APG00065 (58.3%identity, 70.5% similarity) Cry35Ab4 (19.1% identity, 34.1% similarity)APG00227 83 267, 268 APG00517 (79.8% identity, 79.8% similarity) Mtx 55,60, 65, 70, 75, 80, 85, 90, 65, 70, 75, 80, 85, 90, 95, 96, 95, 96, 97,98, 99 97, 98, 99 APG00635 (55.0% identity, 66.2% similarity) APG00020(53.3% identity, 64.4% similarity) WP_002166885.1 (52.2% identity, 61.8%similarity) US20130227743A1_110 (39.4% identity, 54.0% similarity)AGP17985.1 (34.3% identity, 49.0% similarity) US_8829279_B2-11 (30.9%identity, 44.1% similarity) APG00229 84 269, 270 APG00419 (92.6%identity, 94.1% similarity) Bin 70, 75, 80, 85, 90, 95, 96, 97, 80, 85,90, 95, 96, 97, 98, 99 98, 99 APG00065 (86.9% identity, 92.8%similarity) APG00844 (86.4% identity, 89.1% similarity) APG00301 (85.4%identity, 88.4% similarity) APG00412 (84.9% identity, 89.1% similarity)WP_000839920.1 (68.1% identity, 77.5% similarity) WP_002166959.1 (67.6%identity, 77.6% similarity) WP_002191947.1 (67.3% identity, 77.6%similarity) Cry35Ab3 (21.9% identity, 34.9% similarity) APG00230 85 271APG00413 (81.0% identity, 88.9% similarity) Bin 80, 85, 90, 95, 96, 97,98, 99 90, 95, 96, 97, 98, 99 KEZ80012.1 (79.9% identity, 87.6%similarity) WP_017154552.1 (72.3% identity, 83.0% similarity) APG00596(69.1% identity, 80.9% similarity) APG00191 (67.5% identity, 81.0%similarity) APG00741 (65.8% identity, 78.2% similarity) APG00757 (62.3%identity, 73.8% similarity) APG00090 (59.9% identity, 77.2% similarity)US20130227743A1_156 (51.3% identity, 58.4% similarity) Cry35Ad2 (21.3%identity, 36.7% similarity) APG00231 86 272 APG00377 (96.8% identity,98.7% similarity) Bin 90, 95, 96, 97, 98, 99 95, 96, 97, 98, 99 APG00356(95.4% identity, 98.1% similarity) APG00287 (94.4% identity, 97.3%similarity) APG00731 (89.3% identity, 93.6% similarity) APG00035 (87.7%identity, 93.3% similarity) WP_000143307.1 (87.7% identity, 91.7%similarity) WP_000143308.1 (84.5% identity, 91.2% similarity)US20130227743A1_6 (79.4% identity, 83.9% similarity) APG00011 (77.7%identity, 85.5% similarity) Cry35Ab4 (22.4% identity, 39.0% similarity)APG00232 87 273 AGA40057.1 (27.5% identity, 41.5% similarity) Cry 30,35, 40, 45, 50, 55, 60, 65, 45, 50, 55, 60, 65, 70, 75, 80, 70, 75, 80,85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99US20130227743A1_200 (26.7% identity, 41.2% similarity) AGP17992.1 (24.4%identity, 35.9% similarity) Cry73Aa (20.0% identity, 31.5% similarity)APG00233 88 274 APG00251 (81.8% identity, 87.8% similarity) Cry 40, 45,50, 55, 60, 65, 70, 75, 55, 60, 65, 70, 75, 80, 85, 90, 80, 85, 90, 95,96, 97, 98, 99 95, 96, 97, 98, 99 US20130227743A1_200 (38.7% identity,52.6% similarity) AGA40058.1 (25.4% identity, 38.9% similarity)WP_017762619.1 (25.0% identity, 40.7% similarity) Cry41Ba2 (13.1%identity, 20.4% similarity) APG00235 89 275 US20130227743A1_170 (32.8%identity, 37.8% CRY 35, 40, 45, 50, 55, 60, 65, 70, 50, 55, 60, 65, 70,75, 80, 85, similarity) 75, 80, 85, 90, 95, 96, 97, 98, 90, 95, 96, 97,98, 99 99 US20130227743A1_200 (29.8% identity, 41.7% similarity)WP_017762619.1 (29.2% identity, 45.7% similarity) Cyt1Da1 (17.9%identity, 33.1% similarity) APG00237 90 276 APG00221 (88.1% identity,91.6% similarity) Bin 70, 75, 80, 85, 90, 95, 96, 97, 80, 85, 90, 95,96, 97, 98, 99 98, 99 APG00376 (85.3% identity, 89.8% similarity)APG00203 (77.5% identity, 82.8% similarity) APG00844 (77.3% identity,82.6% similarity) WP_002166959.1 (69.1% identity, 78.9% similarity)WP_002191947.1 (68.8% identity, 78.9% similarity) WP_000839920.1 (68.6%identity, 79.6% similarity) APG00065 (65.0% identity, 75.1% similarity)Cry35Ab3 (22.7% identity, 38.7% similarity) APG00239 91 277WP_017762619.1 (27.3% identity, 38.4% similarity) Cry 30, 35, 40, 45,50, 55, 60, 65, 45, 50, 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 95,96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99 WP_017762581.1 (26.9%identity, 37.3% similarity) AGA40058.1 (26.8% identity, 41.8%similarity) AGP17992.1 (26.2% identity, 38.6% similarity) APG00241 92278, 279 KFN03392.1 (55.9% identity, 71.4% similarity) Cry6 60, 65, 70,75, 80, 85, 90, 95, 75, 80, 85, 90, 95, 96, 97, 98, 96, 97, 98, 99 99WP_033674722.1 (55.2% identity, 72.8% similarity) WP_003196831.1 (54.5%identity, 71.2% similarity) WP_018765384.1 (54.5% identity, 71.2%similarity) APG00298 (52.0% identity, 71.0% similarity) APG00242 93 280APG00223 (98.4% identity, 98.6% similarity) Bin 90, 95, 96, 97, 98, 9990, 95, 96, 97, 98, 99 APG00454 (97.9% identity, 98.8% similarity)APG00118 (90.3% identity, 94.7% similarity) US20130227743A1_10 (85.2%identity, 88.4% similarity) WP_001258160.1 (85.0% identity, 88.2%similarity) WP_001258161.1 (84.7% identity, 88.0% similarity) APG00913(84.5% identity, 89.6% similarity) APG00065 (58.8% identity, 71.4%similarity) Cry35Ab3 (19.3% identity, 34.5% similarity) APG00243 94 281APG00301 (86.7% identity, 92.7% similarity) Bin 80, 85, 90, 95, 96, 97,98, 99 85, 90, 95, 96, 97, 98, 99 APG00213 (82.7% identity, 89.1%similarity) APG00844 (82.0% identity, 90.1% similarity) APG00412 (81.6%identity, 89.3% similarity) APG00065 (80.4% identity, 85.1% similarity)WP_000839920.1 (77.4% identity, 84.3% similarity) WP_002166959.1 (73.1%identity, 81.4% similarity) WP_002191947.1 (72.9% identity, 81.4%similarity) Cry35Ad2 (23.0% identity, 37.5% similarity) APG00248 95 282APG00262 (63.2% identity, 73.2% similarity) Bin 60, 65, 70, 75, 80, 85,90, 95, 70, 75, 80, 85, 90, 95, 96, 97, 96, 97, 98, 99 98, 99WP_002191947.1 (59.5% identity, 69.9% similarity) WP_002166959.1 (59.3%identity, 69.9% similarity) APG00243 (59.1% identity, 69.9% similarity)AGA40039.1 (58.0% identity, 69.2% similarity) APG00369 (57.6% identity,68.1% similarity) APG00212 (57.1% identity, 67.6% similarity) APG00065(56.8% identity, 69.6% similarity) Cry35Ab1 (20.6% identity, 33.7%similarity) APG00249 96 283, 284 APG00459 (89.0% identity, 92.8%similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98,99 99 APG00132 (88.9% identity, 94.7% similarity) APG00065 (77.4%identity, 83.8% similarity) APG00407 (75.5% identity, 84.4% similarity)APG00229 (73.0% identity, 81.3% similarity) WP_000839920.1 (71.7%identity, 81.0% similarity) WP_002166959.1 (68.3% identity, 78.0%similarity) WP_002191947.1 (68.0% identity, 78.0% similarity) Cry49Ab1(21.9% identity, 33.7% similarity) APG00251 97 285 APG00233 (81.8%identity, 87.8% similarity) Cry 40, 45, 50, 55, 60, 65, 70, 75, 55, 60,65, 70, 75, 80, 85, 90, 80, 85, 90, 95, 96, 97, 98, 99 95, 96, 97, 98,99 US20130227743A1_200 (37.8% identity, 50.2% similarity) AGA40058.1(26.1% identity, 41.6% similarity) WP_002140071.1 (24.2% identity, 35.0%similarity) Cry3Ca1 (18.9% identity, 30.3% similarity) APG00255 98 286APG00401 (94.1% identity, 96.9% similarity) Cry 35, 40, 45, 50, 55, 60,65, 70, 50, 55, 60, 65, 70, 75, 80, 85, 75, 80, 85, 90, 95, 96, 97, 98,90, 95, 96, 97, 98, 99 99 US20130227743A1_200 (31.3% identity, 46.8%similarity) WP_017762616.1 (28.7% identity, 44.7% similarity) AGA40058.1(28.5% identity, 44.6% similarity) Cry41Aa1 (19.3% identity, 28.9%similarity) APG00258 99 WP_020799009.1 (72.7% identity, 84.5%similarity) Cry6 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98,99 99 WP_037330331.1 (33.2% identity, 47.0% similarity) WP_040871902.1(32.4% identity, 47.5% similarity) Cry6Ba1 (23.6% identity, 40.5%similarity) APG00261 100 287 WP_002187944.1 (76.1% identity, 85.5%similarity) Bin 80, 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99WP_001258160.1 (75.6% identity, 85.0% similarity) WP_001258161.1 (75.6%identity, 85.0% similarity) APG00454 (68.7% identity, 76.8% similarity)APG00242 (68.5% identity, 76.8% similarity) APG00223 (68.5% identity,76.6% similarity) APG00118 (68.1% identity, 76.4% similarity) APG00065(59.2% identity, 69.8% similarity) Cry35Aa2 (21.5% identity, 37.8%similarity) APG00262 101 288, 289 WP_002191947.1 (77.7% identity, 82.3%similarity) Bin 80, 85, 90, 95, 96, 97, 98, 99 85, 90, 95, 96, 97, 98,99 WP_002166959.1 (77.4% identity, 82.3% similarity) APG00925 (73.7%identity, 82.5% similarity) APG00369 (71.9% identity, 80.2% similarity)WP_000839920.1 (71.0% identity, 77.7% similarity) APG00065 (68.7%identity, 77.2% similarity) APG00212 (68.7% identity, 77.1% similarity)APG00249 (67.4% identity, 75.9% similarity) Cry49Aa1 (20.3% identity,33.3% similarity) APG00263 102 WP_037330331.1 (32.9% identity, 51.0%similarity) Cry6 35, 40, 45, 50, 55, 60, 65, 70, 55, 60, 65, 70, 75, 80,85, 90, 75, 80, 85, 90, 95, 96, 97, 98, 95, 96, 97, 98, 99 99WP_040871901.1 (32.9% identity, 49.5% similarity) WP_040871902.1 (31.7%identity, 48.8% similarity) Cry6Ba1 (19.2% identity, 32.2% similarity)APG00264 103 290, 291 APG00307 (66.0% identity, 78.8% similarity) Cry665, 70, 75, 80, 85, 90, 95, 96, 75, 80, 85, 90, 95, 96, 97, 98, 97, 98,99 99 APG00297 (64.5% identity, 73.6% similarity) WP_017762448.1 (64.4%identity, 73.9% similarity) WP_016110403.1 (29.0% identity, 49.5%similarity) WP_002140073.1 (28.9% identity, 49.1% similarity)WP_016083929.1 (26.8% identity, 47.6% similarity) APG00265 104 APG00291(86.4% identity, 91.2% similarity) Cry6 75, 80, 85, 90, 95, 96, 97, 98,85, 90, 95, 96, 97, 98, 99 99 WP_001104853.1 (73.1% identity, 84.5%similarity) WP_002187785.1 (72.1% identity, 84.5% similarity)WP_001104975.1 (71.7% identity, 83.4% similarity) WP_016078425.1 (71.7%identity, 83.4% similarity) APG00280 (63.0% identity, 74.7% similarity)APG00720 (51.2% identity, 74.4% similarity) APG00381 (50.9% identity,73.0% similarity) APG00266 105 292 APG00288 (55.5% identity, 69.9%similarity) Cry 35, 40, 45, 50, 55, 60, 65, 70, 50, 55, 60, 65, 70, 75,80, 85, 75, 80, 85, 90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99 99AGA40058.1 (31.4% identity, 46.0% similarity) AGP17992.1 (27.9%identity, 39.9% similarity) WP_017762581.1 (25.8% identity, 37.3%similarity) WP_017762619.1 (24.9% identity, 36.6% similarity) APG00267106 293, 294 APG00391 (93.5% identity, 96.1% similarity) Cry 30, 35, 40,45, 50, 55, 60, 65, 50, 55, 60, 65, 70, 75, 80, 85, 70, 75, 80, 85, 90,95, 96, 97, 90, 95, 96, 97, 98, 99 98, 99 US20130227743A1_200 (29.7%identity, 46.1% similarity) AGA40058.1 (28.1% identity, 44.7%similarity) WP_017762581.1 (25.9% identity, 39.9% similarity) AGP17992.1(25.2% identity, 37.9% similarity) APG00270 107 WP_007973068.1 (77.4%identity, 90.3% similarity) Cry6 80, 85, 90, 95, 96, 97, 98, 99 95, 96,97, 98, 99 APG00319 (75.8% identity, 89.8% similarity) WP_003379701.1(43.2% identity, 62.2% similarity) WP_017683125.1 (43.2% identity, 62.2%similarity) WP_024677851.1 (43.0% identity, 62.0% similarity) APG00271108 295 WP_018765648.1 (57.1% identity, 74.1% similarity) Cry6 60, 65,70, 75, 80, 85, 90, 95, 75, 80, 85, 90, 95, 96, 97, 98, 96, 97, 98, 9999 WP_018780622.1 (56.7% identity, 74.1% similarity) WP_040119596.1(56.7% identity, 74.1% similarity) WP_000790920.1 (56.5% identity, 74.6%similarity) APG00273 109 296 APG00033 (80.3% identity, 88.1% similarity)Vip 70, 75, 80, 85, 90, 95, 96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98,99 APG00175 (78.0% identity, 85.7% similarity) APG00077 (77.1% identity,84.9% similarity) APG00104 (72.1% identity, 82.1% similarity) KEZ80024.1(69.1% identity, 79.7% similarity) APG00278 (68.9% identity, 78.0%similarity) APG00173 (68.8% identity, 78.1% similarity) US_7129212_B2-17(23.7% identity, 41.2% similarity) US_8334431_B2-13 (23.6% identity,41.2% similarity) Vip3Ca1 (23.0% identity, 37.3% similarity) APG00274110 297, 298 WP_016098095.1 (93.1% identity, 97.1% similarity) Cry6 95,96, 97, 98, 99 98, 99 WP_002064828.1 (77.4% identity, 87.7% similarity)WP_016103147.1 (77.4% identity, 87.7% similarity) WP_002126822.1 (77.1%identity, 87.7% similarity) APG00275 111 299 AGA40057.1 (26.0% identity,30.3% similarity) Cry 30, 35, 40, 45, 50, 55, 60, 65, 35, 40, 45, 50,55, 60, 65, 70, 70, 75, 80, 85, 90, 95, 96, 97, 75, 80, 85, 90, 95, 96,97, 98, 98, 99 99 US20130227743A1_206 (13.3% identity, 20.5% similarity)AGA40058.1 (12.5% identity, 20.2% similarity) WP_017762619.1 (12.4%identity, 18.5% similarity) APG00278 112 113 APG00173 (94.5% identity,96.8% similarity) Vip 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96,97, 98, 99 99 APG00077 (82.3% identity, 88.3% similarity) APG00175(80.7% identity, 86.6% similarity) KEZ80024.1 (73.9% identity, 84.1%similarity) APG00273 (68.9% identity, 78.0% similarity) APG00104 (68.7%identity, 79.0% similarity) APG00033 (68.3% identity, 78.5% similarity)Vip3Ca3 (24.8% identity, 42.0% similarity) APG00279 114 300WP_020799009.1 (33.1% identity, 48.6% similarity) Cry6 35, 40, 45, 50,55, 60, 65, 70, 50, 55, 60, 65, 70, 75, 80, 85, 75, 80, 85, 90, 95, 96,97, 98, 90, 95, 96, 97, 98, 99 99 WP_012620778.1 (29.4% identity, 46.8%similarity) WP_012103144.1 (29.0% identity, 46.2% similarity) Cry6Ba1(23.4% identity, 36.7% similarity) APG00280 115 APG00265 (63.0%identity, 74.7% similarity) Cry6 60, 65, 70, 75, 80, 85, 90, 95, 70, 75,80, 85, 90, 95, 96, 97, 96, 97, 98, 99 98, 99 APG00291 (59.3% identity,71.9% similarity) WP_001104853.1 (55.2% identity, 69.0% similarity)WP_002187785.1 (54.5% identity, 69.7% similarity) WP_001104975.1 (54.5%identity, 69.0% similarity) WP_016078425.1 (53.8% identity, 68.3%similarity) APG00282 116 301 APG00925 (69.9% identity, 75.6% similarity)Bin 65, 70, 75, 80, 85, 90, 95, 96, 75, 80, 85, 90, 95, 96, 97, 98, 97,98, 99 99 APG00369 (66.1% identity, 72.7% similarity) APG00295 (61.3%identity, 68.1% similarity) WP_002191947.1 (61.0% identity, 70.1%similarity) WP_002166959.1 (60.7% identity, 70.1% similarity) APG00262(59.8% identity, 66.6% similarity) WP_016107065.1 (59.0% identity, 69.4%similarity) APG00065 (57.9% identity, 65.8% similarity) Cry35Ab3 (21.5%identity, 36.3% similarity) APG00284 117 302 APG00731 (91.2% identity,96.0% similarity) Bin 90, 95, 96, 97, 98, 99 95, 96, 97, 98, 99WP_000143307.1 (89.3% identity, 94.4% similarity) WP_000143308.1 (89.0%identity, 93.8% similarity) APG00035 (88.7% identity, 95.4% similarity)APG00377 (86.6% identity, 92.5% similarity) APG00231 (86.3% identity,92.2% similarity) APG00356 (85.8% identity, 92.5% similarity)US20130227743A1_6 (76.9% identity, 83.4% similarity) APG00011 (73.7%identity, 84.5% similarity) Cry35Ac2 (22.2% identity, 41.1% similarity)APG00285 118 303 WP_017762581.1 (30.9% identity, 45.1% similarity) Cry35, 40, 45, 50, 55, 60, 65, 70, 50, 55, 60, 65, 70, 75, 80, 85, 75, 80,85, 90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99 99 AGA40057.1 (30.5%identity, 46.6% similarity) AGP17992.1 (30.5% identity, 43.9%similarity) WP_017762616.1 (30.4% identity, 45.2% similarity) APG00286119 120, 304 WP_003204425.1 (53.7% identity, 74.3% similarity) Cry6 55,60, 65, 70, 75, 80, 85, 90, 75, 80, 85, 90, 95, 96, 97, 98, 95, 96, 97,98, 99 99 WP_018767550.1 (53.7% identity, 74.3% similarity)WP_018783608.1 (53.7% identity, 74.0% similarity) WP_003209431.1 (53.5%identity, 74.0% similarity) APG00287 121 305 APG00356 (98.4% identity,98.7% similarity) Bin 90, 95, 96, 97, 98, 99 95, 96, 97, 98, 99 APG00377(95.7% identity, 97.9% similarity) APG00231 (94.4% identity, 97.3%similarity) APG00035 (90.1% identity, 93.8% similarity) APG00731 (89.5%identity, 93.3% similarity) WP_000143307.1 (85.0% identity, 90.6%similarity) WP_000143308.1 (84.2% identity, 90.9% similarity)US20130227743A1_6 (78.8% identity, 83.4% similarity) APG00011 (76.9%identity, 84.7% similarity) Cry35Ab4 (22.5% identity, 39.1% similarity)APG00288 122 306 APG00266 (55.5% identity, 69.9% similarity) Cry 35, 40,45, 50, 55, 60, 65, 70, 50, 55, 60, 65, 70, 75, 80, 85, 75, 80, 85, 90,95, 96, 97, 98, 90, 95, 96, 97, 98, 99 99 AGA40058.1 (31.7% identity,47.3% similarity) AGP17992.1 (28.9% identity, 42.1% similarity)WP_017762581.1 (28.8% identity, 41.8% similarity) AGA40057.1 (26.9%identity, 39.9% similarity) APG00289 123 WP_017683125.1 (47.1% identity,65.9% similarity) Cry6 50, 55, 60, 65, 70, 75, 80, 85, 70, 75, 80, 85,90, 95, 96, 97, 90, 95, 96, 97, 98, 99 98, 99 WP_024677851.1 (47.1%identity, 65.7% similarity) WP_017701944.1 (46.9% identity, 65.9%similarity) WP_011105063.1 (46.9% identity, 65.4% similarity) APG00290124 307 APG00422 (85.0% identity, 86.9% similarity) Cyt 30, 35, 40, 45,50, 55, 60, 65, 40, 45, 50, 55, 60, 65, 70, 75, 70, 75, 80, 85, 90, 95,96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98, 99 APG00698 (82.2% identity,88.2% similarity) APG00647 (81.1% identity, 86.6% similarity) Cyt1Ba1(25.8% identity, 39.2% similarity) APG00291 125 308 APG00265 (86.4%identity, 91.2% similarity) Cry6 75, 80, 85, 90, 95, 96, 97, 98, 85, 90,95, 96, 97, 98, 99 99 WP_001104853.1 (71.6% identity, 82.8% similarity)WP_001104975.1 (70.6% identity, 82.1% similarity) WP_002187785.1 (70.3%identity, 82.8% similarity) WP_016078425.1 (69.9% identity, 82.8%similarity) APG00280 (59.3% identity, 71.9% similarity) APG00381 (50.5%identity, 71.2% similarity) APG00720 (50.2% identity, 73.6% similarity)APG00292 126 309 US20130227743A1_200 (33.4% identity, 48.3% Cry 35, 40,45, 50, 55, 60, 65, 70, 50, 55, 60, 65, 70, 75, 80, 85, similarity) 75,80, 85, 90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99 99 AGA40058.1 (26.5%identity, 42.3% similarity) AGP17992.1 (25.8% identity, 41.0%similarity) Cry42Aa1 (15.7% identity, 25.6% similarity) APG00294 127 310WP_016102361.1 (51.7% identity, 70.6% similarity) Cry6 55, 60, 65, 70,75, 80, 85, 90, 75, 80, 85, 90, 95, 96, 97, 98, 95, 96, 97, 98, 99 99WP_002144067.1 (51.4% identity, 70.4% similarity) WP_002035071.1 (50.0%identity, 69.2% similarity) WP_040119237.1 (49.5% identity, 69.7%similarity) APG00295 128 311, 312 APG00308 (77.6% identity, 82.9%similarity) Bin 65, 70, 75, 80, 85, 90, 95, 96, 75, 80, 85, 90, 95, 96,97, 98, 97, 98, 99 99 APG00385 (70.5% identity, 79.8% similarity)APG00392 (68.9% identity, 79.1% similarity) APG00767 (66.6% identity,76.3% similarity) APG00065 (64.4% identity, 74.3% similarity)WP_033679178.1 (63.7% identity, 74.1% similarity) WP_000839920.1 (62.6%identity, 73.5% similarity) WP_002191947.1 (61.3% identity, 73.2%similarity) Cry49Aa1 (20.5% identity, 34.0% similarity) APG00297 129313, 314 WP_017762448.1 (87.7% identity, 89.1% similarity) Cry6 90, 95,96, 97, 98, 99 90, 95, 96, 97, 98, 99 APG00264 (64.5% identity, 73.6%similarity) APG00307 (63.1% identity, 75.4% similarity) WP_033674645.1(28.2% identity, 44.8% similarity) WP_000751842.1 (27.6% identity, 47.9%similarity) WP_000751829.1 (27.4% identity, 47.9% similarity) APG00298130 315, 316 WP_016098094.1 (94.0% identity, 97.2% similarity) Cry6 95,96, 97, 98, 99 98, 99 KFN03392.1 (77.7% identity, 87.3% similarity)WP_033674722.1 (74.4% identity, 86.8% similarity) WP_002135640.1 (73.1%identity, 85.8% similarity) APG00241 (52.0% identity, 71.0% similarity)APG00301 131 317 APG00353 (93.5% identity, 95.6% similarity) Bin 75, 80,85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98, 99 99 APG00844 (91.1%identity, 94.8% similarity) APG00419 (87.9% identity, 90.4% similarity)APG00412 (87.5% identity, 91.3% similarity) APG00065 (77.7% identity,84.4% similarity) WP_000839920.1 (73.8% identity, 83.0% similarity)WP_002166959.1 (68.8% identity, 79.4% similarity) WP_002114997.1 (67.9%identity, 78.8% similarity) Cry35Ac2 (22.5% identity, 37.8% similarity)APG00302 132 318, 319 AGA40058.1 (27.8% identity, 41.4% similarity) Cry30, 35, 40, 45, 50, 55, 60, 65, 45, 50, 55, 60, 65, 70, 75, 80, 70, 75,80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99US_2013_0227743_A1_178 (27.5% identity, 41.7% similarity)US20130227743A1_200 (26.9% identity, 44.0% similarity) Cry42Aa1 (17.9%identity, 29.2% similarity) APG00305 133 320 WP_017762447.1 (92.7%identity, 96.0% similarity) Cry6 95, 96, 97, 98, 99 97, 98, 99WP_000162958.1 (44.7% identity, 60.9% similarity) WP_002012120.1 (44.7%identity, 60.7% similarity) WP_002118280.1 (44.7% identity, 60.7%similarity) APG00307 134 321, 322 APG00264 (66.0% identity, 78.8%similarity) Cry6 65, 70, 75, 80, 85, 90, 95, 96, 80, 85, 90, 95, 96, 97,98, 99 97, 98, 99 WP_017762448.1 (63.9% identity, 76.3% similarity)APG00297 (63.1% identity, 75.4% similarity) WP_033674645.1 (26.9%identity, 46.1% similarity) KFM95248.1 (26.5% identity, 47.5%similarity) WP_000751870.1 (25.7% identity, 47.0% similarity) APG00308135 323 APG00392 (82.9% identity, 91.2% similarity) Bin 70, 75, 80, 85,90, 95, 96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98, 99 APG00767 (81.8%identity, 89.6% similarity) APG00295 (77.6% identity, 82.9% similarity)APG00385 (76.1% identity, 86.6% similarity) APG00065 (67.0% identity,78.6% similarity) WP_033679178.1 (66.8% identity, 77.4% similarity)WP_000839920.1 (66.8% identity, 77.3% similarity) WP_002191947.1 (64.9%identity, 76.7% similarity) Cry35Ab1 (21.7% identity, 36.6% similarity)APG00314 136 324 WP_029439068.1 (72.5% identity, 81.9% similarity) Bin75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98, 99 99 APG00415(55.1% identity, 66.4% similarity) WP_029439066.1 (46.9% identity, 61.0%similarity) WP_002191947.1 (34.3% identity, 50.9% similarity) Cry35Ac2(22.3% identity, 37.9% similarity) APG00315 137 APG00178 (87.7%identity, 91.6% similarity) Bin 30, 35, 40, 45, 50, 55, 60, 65, 45, 50,55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96,97, 98, 99 98, 99 APG00727 (85.5% identity, 89.9% similarity) Cry49Ab1(27.3% identity, 44.2% similarity) APG00317 138 WP_015096649.1 (68.9%identity, 84.4% similarity) Cry6 70, 75, 80, 85, 90, 95, 96, 97, 85, 90,95, 96, 97, 98, 99 98, 99 APG00400 (68.7% identity, 84.6% similarity)APG00630 (67.2% identity, 83.6% similarity) WP_028939439.1 (45.1%identity, 63.9% similarity) WP_019823923.1 (32.5% identity, 51.3%similarity) WP_016779464.1 (31.5% identity, 51.4% similarity) APG00319139 APG00270 (75.8% identity, 89.8% similarity) Cry6 75, 80, 85, 90, 95,96, 97, 98, 90, 95, 96, 97, 98, 99 99 WP_007973068.1 (71.2% identity,86.8% similarity) WP_024691985.1 (41.9% identity, 62.8% similarity)WP_016569001.1 (41.7% identity, 62.8% similarity) WP_029571787.1 (41.7%identity, 62.8% similarity) APG00320 140 325 APG00913 (75.8% identity,85.4% similarity) Bin 70, 75, 80, 85, 90, 95, 96, 97, 80, 85, 90, 95,96, 97, 98, 99 98, 99 APG00118 (72.2% identity, 81.7% similarity)APG00223 (70.8% identity, 81.7% similarity) APG00398 (68.3% identity,77.3% similarity) WP_002114997.1 (67.6% identity, 75.7% similarity)WP_002187944.1 (61.9% identity, 72.6% similarity) US20130227743A1_10(61.5% identity, 72.6% similarity) APG00065 (54.9% identity, 67.1%similarity) Cry35Ab4 (20.4% identity, 35.2% similarity) APG00340 141 326AGP18023.1 (63.5% identity, 75.5% similarity) Cry 65, 70, 75, 80, 85,90, 95, 96, 80, 85, 90, 95, 96, 97, 98, 99 97, 98, 99 APG00151 (62.2%identity, 74.6% similarity) US20130227743A1_40 (56.7% identity, 65.9%similarity) US20130227743A1_48 (35.7% identity, 45.6% similarity)Cry4Ba4 (25.5% identity, 36.7% similarity) APG00342 142 WP_002144456.1(89.8% identity, 95.8% similarity) Cry6 90, 95, 96, 97, 98, 99 96, 97,98, 99 WP_002169783.1 (86.4% identity, 92.4% similarity) WP_016078427.1(68.0% identity, 82.2% similarity) APG00024 (66.9% identity, 80.8%similarity) APG00180 (62.9% identity, 78.8% similarity) Cry6Ba1 (28.4%identity, 46.7% similarity) APG00349 143 APG00797 (90.2% identity, 94.8%similarity) Bin 45, 50, 55, 60, 65, 70, 75, 80, 65, 70, 75, 80, 85, 90,95, 96, 85, 90, 95, 96, 97, 98, 99 97, 98, 99 WP_000143308.1 (43.0%identity, 61.6% similarity) WP_000143307.1 (42.5% identity, 61.6%similarity) US20130227743A1_6 (40.1% identity, 59.6% similarity)Cry35Ac1 (26.5% identity, 43.3% similarity) APG00353 144 327 APG00203(95.3% identity, 96.6% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98,85, 90, 95, 96, 97, 98, 99 99 APG00301 (93.5% identity, 95.6%similarity) APG00844 (91.1% identity, 94.5% similarity) APG00412 (88.5%identity, 91.8% similarity) APG00065 (76.7% identity, 83.9% similarity)WP_000839920.1 (73.0% identity, 82.7% similarity) WP_002166959.1 (69.3%identity, 80.2% similarity) WP_002114997.1 (67.9% identity, 79.3%similarity) Cry35Ad2 (24.8% identity, 39.6% similarity) APG00355 145 146WP_017762616.1 (23.5% identity, 39.5% similarity) Cry 25, 30, 35, 40,45, 50, 55, 60, 40, 45, 50, 55, 60, 65, 70, 75, 65, 70, 75, 80, 85, 90,95, 96, 80, 85, 90, 95, 96, 97, 98, 99 97, 98, 99 AGA40057.1 (23.1%identity, 35.9% similarity) AGA40058.1 (23.0% identity, 37.4%similarity) WP_017762581.1 (22.5% identity, 36.8% similarity) APG00356147 328 APG00287 (98.4% identity, 98.7% similarity) Bin 90, 95, 96, 97,98, 99 95, 96, 97, 98, 99 APG00377 (97.1% identity, 98.9% similarity)APG00231 (95.4% identity, 98.1% similarity) APG00035 (91.2% identity,94.6% similarity) APG00731 (90.6% identity, 94.4% similarity)WP_000143307.1 (86.1% identity, 91.7% similarity) WP_000143308.1 (84.7%identity, 91.2% similarity) US20130227743A1_6 (79.9% identity, 84.5%similarity) APG00011 (77.7% identity, 85.5% similarity) Cry35Ab4 (22.8%identity, 39.1% similarity) APG00368 148 329 APG00211 (98.7% identity,99.2% similarity) Bin 80, 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98,99 APG00011 (94.9% identity, 96.5% similarity) APG00377 (79.1% identity,86.9% similarity) APG00356 (79.1% identity, 86.4% similarity) APG00231(78.6% identity, 86.4% similarity) APG00035 (77.3% identity, 84.8%similarity) WP_000143307.1 (76.7% identity, 85.0% similarity)WP_000143308.1 (75.4% identity, 84.5% similarity) US20130227743A1_6(69.3% identity, 78.6% similarity) Cry35Ac2 (22.3% identity, 38.6%similarity) APG00369 149 330 APG00925 (79.6% identity, 87.1% similarity)Bin 65, 70, 75, 80, 85, 90, 95, 96, 75, 80, 85, 90, 95, 96, 97, 98, 97,98, 99 99 APG00262 (71.9% identity, 80.2% similarity) APG00282 (66.1%identity, 72.7% similarity) APG00913 (64.9% identity, 77.4% similarity)WP_002191947.1 (62.9% identity, 70.5% similarity) WP_002166959.1 (62.6%identity, 70.5% similarity) APG00065 (61.8% identity, 70.8% similarity)US20130227743A1_148 (59.6% identity, 67.6% similarity) Cry49Aa1 (19.1%identity, 33.5% similarity) APG00372 150 331 AGP17992.1 (36.7% identity,46.7% similarity) Cry 40, 45, 50, 55, 60, 65, 70, 75, 50, 55, 60, 65,70, 75, 80, 85, 80, 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99WP_017762581.1 (36.4% identity, 45.8% similarity) AGA40058.1 (32.6%identity, 48.1% similarity) WP_017762619.1 (30.3% identity, 44.2%similarity) APG00373 151 332 APG00413 (64.7% identity, 74.8% similarity)Bin 65, 70, 75, 80, 85, 90, 95, 96, 80, 85, 90, 95, 96, 97, 98, 99 97,98, 99 WP_017154552.1 (64.2% identity, 75.9% similarity) APG00090 (62.8%identity, 78.5% similarity) KEZ80012.1 (62.5% identity, 73.5%similarity) APG00230 (61.4% identity, 75.2% similarity) APG00596 (61.2%identity, 74.0% similarity) APG00191 (59.8% identity, 72.8% similarity)APG00757 (57.2% identity, 71.8% similarity) WP_003308586.1 (48.6%identity, 62.0% similarity) Cry35Ab1 (21.5% identity, 33.3% similarity)APG00375 152 333 AGA40058.1 (43.6% identity, 59.3% similarity) Cry 45,50, 55, 60, 65, 70, 75, 80, 60, 65, 70, 75, 80, 85, 90, 95, 85, 90, 95,96, 97, 98, 99 96, 97, 98, 99 AGP17992.1 (35.7% identity, 50.5%similarity) WP_017762581.1 (34.7% identity, 48.2% similarity) AGA40057.1(31.7% identity, 45.5% similarity) APG00376 153 334 APG00221 (92.5%identity, 95.1% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90,95, 96, 97, 98, 99 99 APG00237 (85.3% identity, 89.8% similarity)APG00353 (79.4% identity, 85.8% similarity) APG00412 (75.8% identity,81.8% similarity) WP_002166959.1 (70.8% identity, 80.8% similarity)WP_002191947.1 (70.5% identity, 80.8% similarity) WP_000839920.1 (70.1%identity, 81.4% similarity) APG00065 (66.5% identity, 76.3% similarity)Cry35Ac1 (21.3% identity, 36.5% similarity) APG00377 154 335 APG00356(97.1% identity, 98.9% similarity) Bin 90, 95, 96, 97, 98, 99 95, 96,97, 98, 99 APG00231 (96.8% identity, 98.7% similarity) APG00287 (95.7%identity, 97.9% similarity) APG00731 (89.3% identity, 93.6% similarity)APG00035 (88.5% identity, 93.8% similarity) WP_000143307.1 (86.1%identity, 91.2% similarity) WP_000143308.1 (84.7% identity, 90.9%similarity) US20130227743A1_6 (79.6% identity, 83.9% similarity)APG00011 (77.7% identity, 86.1% similarity) Cry35Ab4 (22.5% identity,40.6% similarity) APG00379 155 336, 337 APG00356 (86.9% identity, 93.3%similarity) Bin 80, 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99APG00287 (86.6% identity, 93.3% similarity) APG00231 (86.3% identity,93.0% similarity) APG00377 (86.3% identity, 92.8% similarity) APG00035(80.4% identity, 89.5% similarity) WP_000143308.1 (78.8% identity, 87.4%similarity) WP_000143307.1 (77.2% identity, 86.9% similarity)US20130227743A1_6 (72.7% identity, 81.0% similarity) APG00011 (71.6%identity, 82.6% similarity) Cry35Ab4 (25.2% identity, 42.6% similarity)APG00381 156 APG00397 (92.7% identity, 96.9% similarity) Cry6 85, 90,95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99 APG00720 (80.5% identity,89.9% similarity) WP_001072414.1 (80.1% identity, 88.2% similarity)WP_002144454.1 (78.7% identity, 87.5% similarity) WP_002187783.1 (77.0%identity, 86.4% similarity) WP_002169785.1 (63.8% identity, 77.7%similarity) APG00265 (50.9% identity, 73.0% similarity) APG00291 (50.5%identity, 71.2% similarity) APG00385 157 338 APG00392 (80.2% identity,89.9% similarity) Bin 70, 75, 80, 85, 90, 95, 96, 97, 80, 85, 90, 95,96, 97, 98, 99 98, 99 APG00767 (77.0% identity, 86.2% similarity)APG00407 (76.9% identity, 85.9% similarity) APG00308 (76.1% identity,86.6% similarity) APG00065 (67.8% identity, 78.8% similarity)WP_002166959.1 (67.4% identity, 77.8% similarity) WP_002191947.1 (67.2%identity, 77.8% similarity) WP_000839920.1 (67.1% identity, 78.1%similarity) Cry35Ad2 (23.9% identity, 39.2% similarity) APG00386 158WP_002167240.1 (88.1% identity, 91.4% similarity) Bin 90, 95, 96, 97,98, 99 95, 96, 97, 98, 99 WP_016093722.1 (62.3% identity, 75.9%similarity) APG00215 (59.0% identity, 73.7% similarity) WP_002090518.1(56.1% identity, 70.4% similarity) Cry35Ab1 (23.9% identity, 44.8%similarity) APG00388 159 WP_011898730.1 (43.7% identity, 60.0%similarity) Cyt 45, 50, 55, 60, 65, 70, 75, 80, 65, 70, 75, 80, 85, 90,95, 96, 85, 90, 95, 96, 97, 98, 99 97, 98, 99 WP_005311350.1 (43.2%identity, 59.5% similarity) WP_017413134.1 (43.2% identity, 59.5%similarity) Cyt1Aa6 (30.5% identity, 51.6% similarity) APG00391 160 339APG00267 (93.5% identity, 96.1% similarity) Cry 30, 35, 40, 45, 50, 55,60, 65, 50, 55, 60, 65, 70, 75, 80, 85, 70, 75, 80, 85, 90, 95, 96, 97,90, 95, 96, 97, 98, 99 98, 99 US20130227743A1_200 (29.7% identity, 46.5%similarity) AGA40058.1 (28.0% identity, 45.5% similarity) WP_017762581.1(25.8% identity, 40.4% similarity) AGP17992.1 (25.5% identity, 39.0%similarity) APG00392 161 340 APG00308 (82.9% identity, 91.2% similarity)Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98, 99 99APG00407 (82.1% identity, 88.3% similarity) APG00385 (80.2% identity,89.9% similarity) APG00767 (79.0% identity, 88.2% similarity) APG00065(73.4% identity, 83.7% similarity) US20130227743A1_146 (72.5% identity,81.0% similarity) WP_000839920.1 (70.4% identity, 80.1% similarity)WP_002166959.1 (69.9% identity, 79.2% similarity) Cry49Aa1 (21.3%identity, 32.5% similarity) APG00395 162 341 AGA40057.1 (27.9% identity,41.3% similarity) Cry 30, 35, 40, 45, 50, 55, 60, 65, 50, 55, 60, 65,70, 75, 80, 85, 70, 75, 80, 85, 90, 95, 96, 97, 90, 95, 96, 97, 98, 9998, 99 AGP17992.1 (27.5% identity, 40.9% similarity) WP_017762581.1(27.4% identity, 41.4% similarity) AGA40058.1 (26.6% identity, 45.5%similarity) APG00396 163 342 AGA40057.1 (29.5% identity, 43.1%similarity) Cry 30, 35, 40, 45, 50, 55, 60, 65, 45, 50, 55, 60, 65, 70,75, 80, 70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98,99 AGP17992.1 (28.6% identity, 42.9% similarity) WP_017762581.1 (28.1%identity, 40.8% similarity) US_2013_0227743_A1_178 (27.0% identity,39.7% similarity) APG00397 164 APG00381 (92.7% identity, 96.9%similarity) Cry6 80, 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99APG00720 (79.4% identity, 89.2% similarity) WP_001072414.1 (79.1%identity, 87.1% similarity) WP_002144454.1 (77.4% identity, 86.4%similarity) WP_002187783.1 (76.0% identity, 86.1% similarity)WP_002169785.1 (63.4% identity, 77.4% similarity) APG00398 165 343, 344APG00913 (85.9% identity, 88.4% similarity) Bin 75, 80, 85, 90, 95, 96,97, 98, 80, 85, 90, 95, 96, 97, 98, 99 99 APG00118 (80.5% identity,83.9% similarity) APG00223 (78.0% identity, 83.0% similarity) APG00454(77.4% identity, 82.8% similarity) WP_002187944.1 (70.9% identity, 74.9%similarity) US20130227743A1_10 (70.2% identity, 75.5% similarity)WP_001258160.1 (70.0% identity, 75.3% similarity) APG00065 (56.6%identity, 67.6% similarity) Cry49Ab1 (18.7% identity, 31.1% similarity)APG00399 166 WP_023520621.1 (28.4% identity, 43.8% similarity) Bin 30,35, 40, 45, 50, 55, 60, 65, 45, 50, 55, 60, 65, 70, 75, 80, 70, 75, 80,85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99 WP_010720814.1(25.1% identity, 36.9% similarity) WP_010829185.1 (15.6% identity, 25.4%similarity) Cry41Aa1 (13.1% identity, 21.9% similarity) APG00400 167APG00630 (90.7% identity, 95.4% similarity) Cry6 95, 96, 97, 98, 99 96,97, 98, 99 WP_015096649.1 (90.5% identity, 95.8% similarity) APG00317(68.7% identity, 84.6% similarity) WP_028939439.1 (42.4% identity, 60.4%similarity) WP_019823923.1 (32.3% identity, 54.2% similarity)WP_016779464.1 (31.5% identity, 52.8% similarity) APG00401 168 345APG00255 (94.1% identity, 96.9% similarity) Cry 35, 40, 45, 50, 55, 60,65, 70, 50, 55, 60, 65, 70, 75, 80, 85, 75, 80, 85, 90, 95, 96, 97, 98,90, 95, 96, 97, 98, 99 99 US20130227743A1_200 (32.4% identity, 48.3%similarity) WP_017762616.1 (29.8% identity, 45.1% similarity) AGA40058.1(29.6% identity, 44.4% similarity) Cry42Aa1 (18.9% identity, 30.2%similarity) APG00404 169 WP_017762616.1 (30.6% identity, 45.0%similarity) Cry 35, 40, 45, 50, 55, 60, 65, 70, 50, 55, 60, 65, 70, 75,80, 85, 75, 80, 85, 90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99 99AGA40057.1 (28.3% identity, 41.0% similarity) AGA40058.1 (27.0%identity, 42.3% similarity) WP_017762581.1 (25.7% identity, 41.1%similarity) APG00407 170 346 APG00392 (82.1% identity, 88.3% similarity)Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98, 99 99APG00065 (77.2% identity, 84.0% similarity) APG00385 (76.9% identity,85.9% similarity) APG00132 (75.9% identity, 85.2% similarity) APG00249(75.5% identity, 84.4% similarity) US20130227743A1_146 (71.0% identity,79.7% similarity) WP_000839920.1 (70.8% identity, 80.8% similarity)WP_002166959.1 (70.5% identity, 79.2% similarity) Cry35Ad1 (21.1%identity, 35.4% similarity) APG00408 171 APG00067 (81.6% identity, 89.3%similarity) Mtx 70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98,99 98, 99 APG00559 (72.5% identity, 79.5% similarity)US20130227743A1_100 (69.9% identity, 80.9% similarity) WP_000963933.1(69.7% identity, 81.0% similarity) APG00006 (68.3% identity, 80.1%similarity) APG00201 (68.3% identity, 78.1% similarity) APG00036 (67.4%identity, 78.1% similarity) APG00137 (66.2% identity, 78.0% similarity)APG00898 (65.9% identity, 79.5% similarity) APG00022 (64.2% identity,77.2% similarity) US20130227743A1_60 (37.2% identity, 43.7% similarity)WP_026324166.1 (24.1% identity, 38.7% similarity) APG00409 172 347WP_016576337.1 (42.8% identity, 53.8% similarity) Cyt 45, 50, 55, 60,65, 70, 75, 80, 55, 60, 65, 70, 75, 80, 85, 90, 85, 90, 95, 96, 97, 98,99 95, 96, 97, 98, 99 WP_028812057.1 (42.4% identity, 52.3% similarity)WP_039629475.1 (42.0% identity, 54.2% similarity) Cyt2Aa2 (21.3%identity, 39.9% similarity) APG00412 173 348, 349 APG00844 (88.5%identity, 92.6% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90,95, 96, 97, 98, 99 99 APG00353 (88.5% identity, 91.8% similarity)APG00301 (87.5% identity, 91.3% similarity) APG00229 (84.9% identity,89.1% similarity) APG00065 (75.7% identity, 84.4% similarity)WP_000839920.1 (70.4% identity, 81.1% similarity) WP_002166959.1 (67.6%identity, 78.0% similarity) WP_002191947.1 (67.3% identity, 78.0%similarity) Cry35Ab3 (23.4% identity, 37.7% similarity) APG00413 174 350KEZ80012.1 (90.4% identity, 94.4% similarity) Bin 95, 96, 97, 98, 99 95,96, 97, 98, 99 APG00230 (81.0% identity, 88.9% similarity)WP_017154552.1 (73.5% identity, 83.6% similarity) APG00596 (69.6%identity, 82.2% similarity) APG00741 (69.0% identity, 79.3% similarity)APG00191 (66.2% identity, 79.2% similarity) APG00757 (64.8% identity,74.1% similarity) APG00090 (63.7% identity, 79.3% similarity)US20130227743A1_156 (55.3% identity, 60.3% similarity) APG00060 (52.2%identity, 59.9% similarity) Cry35Ac2 (22.1% identity, 37.7% similarity)APG00415 175 351 WP_029439066.1 (68.8% identity, 81.5% similarity) Bin70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99WP_029439068.1 (58.2% identity, 74.4% similarity) APG00314 (55.1%identity, 66.4% similarity) WP_033679178.1 (40.2% identity, 56.5%similarity) Cry35Ac2 (25.4% identity, 44.5% similarity) APG00419 176 352APG00229 (92.6% identity, 94.1% similarity) Bin 70, 75, 80, 85, 90, 95,96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98, 99 APG00844 (88.4% identity,90.9% similarity) APG00353 (88.2% identity, 90.4% similarity) APG00301(87.9% identity, 90.4% similarity) APG00065 (84.1% identity, 90.4%similarity) WP_000839920.1 (69.0% identity, 78.4% similarity)WP_002166959.1 (68.7% identity, 77.9% similarity) WP_002191947.1 (68.4%identity, 77.9% similarity) Cry35Ab5 (21.6% identity, 36.0% similarity)APG00422 177 APG00290 (85.0% identity, 86.9% similarity) Cyt 30, 35, 40,45, 50, 55, 60, 65, 40, 45, 50, 55, 60, 65, 70, 75, 70, 75, 80, 85, 90,95, 96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98, 99 APG00647 (78.0%identity, 83.3% similarity) APG00698 (77.5% identity, 83.0% similarity)Cyt1Aa6 (26.0% identity, 39.0% similarity) WP_000429377.1 (26.0%identity, 39.0% similarity) APG00427 178 353 APG00851 (88.4% identity,93.1% similarity) Mtx 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99WP_018673409.1 (80.7% identity, 89.3% similarity) WP_001039209.1 (68.4%identity, 81.0% similarity) US_8829279_B2-2 (55.7% identity, 70.6%similarity) US_8829279_B2-61 (55.7% identity, 70.6% similarity) APG00454179 354 APG00242 (97.9% identity, 98.8% similarity) Bin 85, 90, 95, 96,97, 98, 99 90, 95, 96, 97, 98, 99 APG00223 (97.7% identity, 98.4%similarity) APG00118 (90.3% identity, 94.9% similarity)US20130227743A1_10 (84.7% identity, 88.4% similarity) APG00913 (84.5%identity, 89.8% similarity) WP_001258160.1 (84.5% identity, 88.2%similarity) WP_001258161.1 (84.3% identity, 88.0% similarity) APG00065(58.1% identity, 70.5% similarity) Cry35Ab4 (19.5% identity, 34.9%similarity) APG00456 180 WP_003203846.1 (94.1% identity, 95.6%similarity) Cyt 95, 96, 97, 98, 99 96, 97, 98, 99 WP_033798332.1 (94.1%identity, 95.6% similarity) WP_040119538.1 (94.1% identity, 95.6%similarity) Cyt2Ca1 (24.8% identity, 42.7% similarity) APG00459 181 355APG00249 (89.0% identity, 92.8% similarity) Bin 75, 80, 85, 90, 95, 96,97, 98, 85, 90, 95, 96, 97, 98, 99 99 APG00132 (88.0% identity, 94.1%similarity) APG00065 (77.5% identity, 83.9% similarity) APG00419 (75.5%identity, 84.6% similarity) APG00407 (75.3% identity, 85.6% similarity)WP_000839920.1 (73.4% identity, 82.3% similarity) WP_002166959.1 (71.3%identity, 80.0% similarity) WP_002191947.1 (71.0% identity, 80.0%similarity) Cry35Ab4 (20.3% identity, 34.4% similarity) APG00461 182APG00050 (64.2% identity, 74.1% similarity) Bin 30, 35, 40, 45, 50, 55,60, 65, 45, 50, 55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 95, 96, 97,85, 90, 95, 96, 97, 98, 99 98, 99 US_7692068_B2-2 (29.9% identity, 40.6%similarity) Cry49Aa1 (29.2% identity, 41.4% similarity) APG00462 183WP_017413134.1 (85.4% identity, 92.1% similarity) Cyt 90, 95, 96, 97,98, 99 95, 96, 97, 98, 99 WP_005311350.1 (85.0% identity, 91.7%similarity) AJE36343.1 (84.9% identity, 92.9% similarity) Cyt1Aa6 (33.3%identity, 48.0% similarity) APG00467 184 WP_017762581.1 (32.2% identity,42.6% similarity) Cry 35, 40, 45, 50, 55, 60, 65, 70, 45, 50, 55, 60,65, 70, 75, 80, 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96, 97, 98,99 99 AGP17992.1 (31.9% identity, 43.9% similarity) US20130227743A1_184(29.7% identity, 44.1% similarity) AGA40058.1 (27.5% identity, 43.0%similarity) APG00499 185 356 APG00532 (65.3% identity, 75.5% similarity)Bin 60, 65, 70, 75, 80, 85, 90, 95, 70, 75, 80, 85, 90, 95, 96, 97, 96,97, 98, 99 98, 99 WP_003308586.1 (58.1% identity, 69.5% similarity)EJR24476.1 (53.9% identity, 59.5% similarity) KEZ80012.1 (48.5%identity, 60.7% similarity) Cry35Aa2 (19.4% identity, 34.1% similarity)APG00500 186 357 AGP17990.1 (63.6% identity, 75.3% similarity) Cry 65,70, 75, 80, 85, 90, 95, 96, 80, 85, 90, 95, 96, 97, 98, 99 97, 98, 99APG00030 (57.5% identity, 64.9% similarity) APG00113 (56.6% identity,65.3% similarity) APG00012 (56.3% identity, 58.2% similarity) APG00096(51.8% identity, 60.6% similarity) APG00196 (51.7% identity, 62.1%similarity) AGP17989.1 (51.3% identity, 60.8% similarity)US_2013_0227743_A1_194 (50.8% identity, 58.2% similarity) Cry70Ba1(23.3% identity, 37.3% similarity) APG00517 187 358 APG00227 (79.8%identity, 79.8% similarity) Mtx 45, 50, 55, 60, 65, 70, 75, 80, 50, 55,60, 65, 70, 75, 80, 85, 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98,99 WP_002166885.1 (40.6% identity, 49.8% similarity) US20130227743A1_110(33.6% identity, 47.5% similarity) AGP17985.1 (28.6% identity, 42.5%similarity) US_8829279_B2-11 (28.4% identity, 41.1% similarity) APG00521188 359 WP_000839061.1 (69.5% identity, 81.5% similarity) Bin 70, 75,80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99US20130227743A1_146 (65.0% identity, 77.2% similarity) WP_002166959.1(63.2% identity, 73.9% similarity) APG00243 (63.1% identity, 74.4%similarity) APG00065 (62.9% identity, 75.4% similarity) APG00132 (62.9%identity, 74.5% similarity) APG00249 (62.4% identity, 73.0% similarity)APG00212 (59.4% identity, 71.7% similarity) Cry35Aa1 (22.3% identity,38.1% similarity) APG00532 189 APG00499 (65.3% identity, 75.5%similarity) Bin 65, 70, 75, 80, 85, 90, 95, 96, 80, 85, 90, 95, 96, 97,98, 99 97, 98, 99 WP_003308586.1 (64.7% identity, 76.5% similarity)WP_017154552.1 (52.3% identity, 66.7% similarity) KEZ80012.1 (51.7%identity, 65.8% similarity) APG00230 (50.6% identity, 66.0% similarity)Cry35Aa2 (22.6% identity, 38.5% similarity) APG00550 190 WP_000727408.1(41.4% identity, 56.0% similarity) Bin 45, 50, 55, 60, 65, 70, 75, 80,60, 65, 70, 75, 80, 85, 90, 95, 85, 90, 95, 96, 97, 98, 99 96, 97, 98,99 WP_000727409.1 (41.4% identity, 56.0% similarity) WP_016110923.1(41.4% identity, 56.0% similarity) Cry35Ad2 (22.3% identity, 41.8%similarity) APG00559 191 360, 361 APG00067 (75.5% identity, 81.2%similarity) Mtx 70, 75, 80, 85, 90, 95, 96, 97, 80, 85, 90, 95, 96, 97,98, 99 98, 99 APG00408 (72.5% identity, 79.5% similarity) WP_000963933.1(66.0% identity, 76.8% similarity) US20130227743A1_100 (65.6% identity,75.6% similarity) APG00006 (63.6% identity, 74.4% similarity) APG00137(63.5% identity, 75.7% similarity) APG00036 (63.2% identity, 73.6%similarity) APG00201 (63.2% identity, 73.0% similarity) APG00898 (62.8%identity, 73.8% similarity) APG00022 (60.8% identity, 73.8% similarity)US20130227743A1_60 (33.4% identity, 40.0% similarity) WP_037756193.1(24.6% identity, 35.2% similarity) APG00571 192 362 APG00377 (76.5%identity, 84.7% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90,95, 96, 97, 98, 99 99 APG00356 (76.5% identity, 83.6% similarity)APG00731 (75.7% identity, 80.7% similarity) APG00035 (74.7% identity,81.5% similarity) APG00284 (74.0% identity, 81.7% similarity)WP_000143307.1 (72.9% identity, 79.1% similarity) WP_000143308.1 (72.1%identity, 80.4% similarity) US20130227743A1_6 (65.8% identity, 73.9%similarity) APG00011 (64.4% identity, 73.5% similarity) Cry35Ac2 (24.8%identity, 42.9% similarity) APG00590 193 363 AGA40045.1 (73.7% identity,82.6% similarity) Mtx 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96,97, 98, 99 99 APG00146 (68.1% identity, 79.8% similarity) WP_000794514.1(67.8% identity, 80.4% similarity) US20130227743A1_102 (61.7% identity,74.8% similarity) WP_036654376.1 (44.3% identity, 59.9% similarity)APG00591 194 WP_033679178.1 (48.6% identity, 59.9% similarity) Bin 50,55, 60, 65, 70, 75, 80, 85, 65, 70, 75, 80, 85, 90, 95, 96, 90, 95, 96,97, 98, 99 97, 98, 99 WP_002166959.1 (48.5% identity, 61.2% similarity)WP_002191947.1 (48.2% identity, 61.2% similarity) Cry35Ac2 (27.3%identity, 45.9% similarity) APG00592 195 364, 365 APG00212 (89.8%identity, 94.2% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90,95, 96, 97, 98, 99 99 APG00798 (86.2% identity, 91.7% similarity)APG00619 (84.7% identity, 89.8% similarity) APG00600 (83.9% identity,90.0% similarity) US20130227743A1_146 (74.9% identity, 82.6% similarity)APG00065 (68.9% identity, 77.2% similarity) WP_002166959.1 (67.1%identity, 76.8% similarity) WP_002191947.1 (66.8% identity, 76.8%similarity) Cry49Aa1 (22.1% identity, 35.4% similarity) APG00596 196 366WP_017154552.1 (88.2% identity, 93.7% similarity) Bin 90, 95, 96, 97,98, 99 95, 96, 97, 98, 99 APG00741 (71.7% identity, 81.1% similarity)APG00413 (69.6% identity, 82.2% similarity) KEZ80012.1 (69.2% identity,80.5% similarity) APG00230 (69.1% identity, 80.9% similarity) APG00757(66.7% identity, 76.0% similarity) APG00191 (60.6% identity, 75.7%similarity) APG00090 (57.7% identity, 74.3% similarity) APG00060 (56.5%identity, 61.5% similarity) US20130227743A1_156 (55.1% identity, 61.9%similarity) Cry49Aa1 (19.3% identity, 30.8% similarity) APG00600 197 367APG00212 (87.1% identity, 92.0% similarity) Bin 75, 80, 85, 90, 95, 96,97, 98, 85, 90, 95, 96, 97, 98, 99 99 APG00619 (85.6% identity, 93.3%similarity) APG00798 (84.7% identity, 90.8% similarity) APG00592 (83.9%identity, 90.0% similarity) US20130227743A1_146 (73.5% identity, 82.4%similarity) WP_002166959.1 (68.5% identity, 78.2% similarity)WP_002191947.1 (68.2% identity, 78.2% similarity) APG00065 (63.3%identity, 75.2% similarity) Cry35Ab3 (22.3% identity, 37.0% similarity)APG00603 198 WP_017762616.1 (32.7% identity, 49.5% similarity) Cry 35,40, 45, 50, 55, 60, 65, 70, 50, 55, 60, 65, 70, 75, 80, 85, 75, 80, 85,90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99 99 AGA40058.1 (28.4%identity, 41.3% similarity) US20130227743A1_206 (25.2% identity, 39.8%similarity) WP_017762619.1 (24.6% identity, 40.7% similarity) APG00619199 368 APG00798 (88.6% identity, 92.7% similarity) Bin 75, 80, 85, 90,95, 96, 97, 98, 85, 90, 95, 96, 97, 98, 99 99 APG00212 (86.2% identity,92.0% similarity) APG00600 (85.6% identity, 93.3% similarity) APG00592(84.7% identity, 89.8% similarity) US20130227743A1_146 (73.6% identity,82.3% similarity) WP_002166959.1 (68.8% identity, 78.6% similarity)WP_002191947.1 (68.6% identity, 78.6% similarity) APG00065 (65.6%identity, 75.5% similarity) Cry35Ab4 (20.6% identity, 36.5% similarity)APG00630 200 APG00400 (90.7% identity, 95.4% similarity) Cry6 90, 95,96, 97, 98, 99 95, 96, 97, 98, 99 WP_015096649.1 (87.5% identity, 93.9%similarity) APG00317 (67.2% identity, 83.6% similarity) WP_028939439.1(41.9% identity, 59.9% similarity) WP_019823923.1 (32.4% identity, 52.1%similarity) WP_016779464.1 (30.4% identity, 52.1% similarity) APG00635201 369, 370, 371 APG00020 (81.9% identity, 86.7% similarity) Mtx 80,85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99 WP_002166885.1 (79.8%identity, 85.4% similarity) APG00227 (55.0% identity, 66.2% similarity)US20130227743A1_110 (44.0% identity, 60.2% similarity) AGP17985.1 (36.1%identity, 51.4% similarity) AGP17983.1 (34.9% identity, 52.1%similarity) APG00642 202 203 WP_029439068.1 (34.5% identity, 48.8%similarity) Bin 35, 40, 45, 50, 55, 60, 65, 70, 50, 55, 60, 65, 70, 75,80, 85, 75, 80, 85, 90, 95, 96, 97, 98, 90, 95, 96, 97, 98, 99 99WP_033679178.1 (33.9% identity, 47.6% similarity) R8EAW2_BACCE (32.6%identity, 47.2% similarity) Cry35Aa1 (22.1% identity, 36.9% similarity)APG00647 204 372 APG00698 (85.4% identity, 91.2% similarity) Cyt 25, 30,35, 40, 45, 50, 55, 60, 40, 45, 50, 55, 60, 65, 70, 75, 65, 70, 75, 80,85, 90, 95, 96, 80, 85, 90, 95, 96, 97, 98, 99 97, 98, 99 APG00290(81.1% identity, 86.6% similarity) APG00422 (78.0% identity, 83.3%similarity) GAJ51067.1 (24.9% identity, 35.1% similarity) Cyt1Ba1 (24.6%identity, 38.6% similarity) APG00652 205 373 WP_026592789.1 (50.4%identity, 68.7% similarity) Cry6 55, 60, 65, 70, 75, 80, 85, 90, 70, 75,80, 85, 90, 95, 96, 97, 95, 96, 97, 98, 99 98, 99 WP_002175239.1 (49.9%identity, 69.7% similarity) WP_000591964.1 (49.3% identity, 69.5%similarity) WP_000591974.1 (49.3% identity, 69.5% similarity) APG00698206 374 APG00647 (85.4% identity, 91.2% similarity) Cyt 25, 30, 35, 40,45, 50, 55, 60, 40, 45, 50, 55, 60, 65, 70, 75, 65, 70, 75, 80, 85, 90,95, 96, 80, 85, 90, 95, 96, 97, 98, 99 97, 98, 99 APG00290 (82.2%identity, 88.2% similarity) APG00422 (77.5% identity, 83.0% similarity)Cyt1Ba1 (24.5% identity, 38.3% similarity) APG00700 207 APG00095 (97.9%identity, 98.2% similarity) Bin 30, 35, 40, 45, 50, 55, 60, 65, 45, 50,55, 60, 65, 70, 75, 80, 70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96,97, 98, 99 98, 99 Cry49Ab1 (28.7% identity, 40.9% similarity) APG00720208 WP_001072414.1 (88.5% identity, 94.1% similarity) Cry6 90, 95, 96,97, 98, 99 95, 96, 97, 98, 99 WP_002187783.1 (87.1% identity, 93.7%similarity) WP_002144454.1 (86.4% identity, 94.1% similarity) APG00381(80.5% identity, 89.9% similarity) APG00397 (79.4% identity, 89.2%similarity) WP_002169785.1 (67.0% identity, 79.4% similarity) APG00265(51.2% identity, 74.4% similarity) APG00291 (50.2% identity, 73.6%similarity) APG00723 209 375 WP_017762581.1 (28.9% identity, 41.0%similarity) Cry 30, 35, 40, 45, 50, 55, 60, 65, 45, 50, 55, 60, 65, 70,75, 80, 70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98,99 AGA40057.1 (28.7% identity, 42.2% similarity) AGA40058.1 (27.8%identity, 40.4% similarity) AGP17992.1 (27.8% identity, 40.0%similarity) APG00727 210 APG00178 (90.7% identity, 93.6% similarity) Bin30, 35, 40, 45, 50, 55, 60, 65, 45, 50, 55, 60, 65, 70, 75, 80, 70, 75,80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99 APG00315(85.5% identity, 89.9% similarity) Cry49Aa1 (26.8% identity, 41.9%similarity) APG00731 211 376 APG00035 (95.4% identity, 98.4% similarity)Bin 95, 96, 97, 98, 99 98, 99 WP_000143307.1 (93.8% identity, 97.1%similarity) APG00284 (91.2% identity, 96.0% similarity) APG00356 (90.6%identity, 94.4% similarity) WP_000143308.1 (90.1% identity, 96.0%similarity) APG00287 (89.5% identity, 93.3% similarity) APG00231 (89.3%identity, 93.6% similarity) US20130227743A1_6 (82.6% identity, 86.6%similarity) APG00011 (76.4% identity, 85.3% similarity) Cry35Ac2 (21.1%identity, 40.5% similarity) APG00732 212 WP_016084067.1 (45.6% identity,53.2% similarity) BIN 50, 55, 60, 65, 70, 75, 80, 85, 55, 60, 65, 70,75, 80, 85, 90, 90, 95, 96, 97, 98, 99 95, 96, 97, 98, 99 WP_000836979.1(45.4% identity, 53.6% similarity) WP_016123021.1 (36.1% identity, 47.0%similarity) Cry73Aa (16.7% identity, 26.2% similarity) APG00741 213APG00757 (91.4% identity, 92.2% similarity) Bin 75, 80, 85, 90, 95, 96,97, 98, 85, 90, 95, 96, 97, 98, 99 99 WP_017154552.1 (73.7% identity,82.5% similarity) APG00060 (72.2% identity, 72.5% similarity) APG00596(71.7% identity, 81.1% similarity) APG00413 (69.0% identity, 79.3%similarity) KEZ80012.1 (66.7% identity, 76.9% similarity) APG00230(65.8% identity, 78.2% similarity) APG00090 (63.0% identity, 76.1%similarity) APG00191 (62.6% identity, 76.4% similarity) WP_003308586.1(49.5% identity, 62.4% similarity) Cry49Aa1 (22.4% identity, 34.6%similarity) APG00748 214 377 WP_002016877.1 (43.1% identity, 61.8%similarity) Bin 45, 50, 55, 60, 65, 70, 75, 80, 65, 70, 75, 80, 85, 90,95, 96, 85, 90, 95, 96, 97, 98, 99 97, 98, 99 WP_016097060.1 (42.0%identity, 59.4% similarity) WP_002167240.1 (40.5% identity, 56.5%similarity) Cry35Ad1 (22.3% identity, 37.4% similarity) APG00757 215 378APG00741 (91.4% identity, 92.2% similarity) Bin 70, 75, 80, 85, 90, 95,96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98, 99 APG00060 (69.4% identity,69.4% similarity) WP_017154552.1 (68.8% identity, 77.3% similarity)APG00596 (66.7% identity, 76.0% similarity) APG00413 (64.8% identity,74.1% similarity) KEZ80012.1 (63.2% identity, 73.1% similarity) APG00230(62.3% identity, 73.8% similarity) APG00090 (59.0% identity, 72.0%similarity) APG00191 (58.6% identity, 71.7% similarity) WP_003308586.1(49.8% identity, 63.6% similarity) Cry49Aa1 (21.1% identity, 32.6%similarity) APG00767 216 379 APG00308 (81.8% identity, 89.6% similarity)Bin 70, 75, 80, 85, 90, 95, 96, 97, 80, 85, 90, 95, 96, 97, 98, 99 98,99 APG00392 (79.0% identity, 88.2% similarity) APG00385 (77.0% identity,86.2% similarity) APG00407 (71.4% identity, 81.9% similarity)WP_000839920.1 (65.0% identity, 76.3% similarity) WP_002166959.1 (63.1%identity, 75.2% similarity) WP_002191947.1 (62.9% identity, 75.2%similarity) APG00065 (62.4% identity, 76.3% similarity) Cry35Ab1 (20.9%identity, 36.5% similarity) APG00768 217 380 WP_000875422.1 (67.7%identity, 81.0% similarity) Mtx 70, 75, 80, 85, 90, 95, 96, 97, 85, 90,95, 96, 97, 98, 99 98, 99 US20130227743A1_58 (67.1% identity, 81.5%similarity) WP_000875423.1 (67.1% identity, 80.7% similarity)WP_000797173.1 (66.7% identity, 81.0% similarity) APG00055 (65.0%identity, 80.6% similarity) APG00174 (61.7% identity, 75.0% similarity)APG00064 (57.9% identity, 73.5% similarity) APG00797 218 APG00349 (90.2%identity, 94.8% similarity) Bin 45, 50, 55, 60, 65, 70, 75, 80, 65, 70,75, 80, 85, 90, 95, 96, 85, 90, 95, 96, 97, 98, 99 97, 98, 99WP_000143308.1 (43.6% identity, 61.7% similarity) WP_000143307.1 (42.8%identity, 61.7% similarity) US20130227743A1_6 (40.2% identity, 59.8%similarity) Cry35Ac1 (27.1% identity, 43.8% similarity) APG00798 219381, 382 APG00212 (92.7% identity, 96.8% similarity) Bin 75, 80, 85, 90,95, 96, 97, 98, 85, 90, 95, 96, 97, 98, 99 99 APG00619 (88.6% identity,92.7% similarity) APG00592 (86.2% identity, 91.7% similarity) APG00600(84.7% identity, 90.8% similarity) US20130227743A1_146 (71.8% identity,80.3% similarity) APG00065 (68.0% identity, 77.6% similarity)WP_002166959.1 (67.1% identity, 77.1% similarity) WP_002191947.1 (66.9%identity, 77.1% similarity) Cry35Ab4 (22.1% identity, 35.7% similarity)APG00830 220 383, 384 WP_033678680.1 (58.7% identity, 74.6% similarity)Cry6 60, 65, 70, 75, 80, 85, 90, 95, 75, 80, 85, 90, 95, 96, 97, 98, 96,97, 98, 99 99 WP_002120795.1 (58.7% identity, 73.7% similarity)WP_002161211.1 (58.7% identity, 73.4% similarity) WP_002199893.1 (58.5%identity, 73.7% similarity) APG00844 221 385 APG00353 (91.1% identity,94.5% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 85, 90, 95, 96,97, 98, 99 99 APG00412 (88.5% identity, 92.6% similarity) APG00419(88.4% identity, 90.9% similarity) APG00229 (86.4% identity, 89.1%similarity) APG00065 (78.7% identity, 86.6% similarity) WP_000839920.1(72.5% identity, 82.4% similarity) WP_002166959.1 (70.1% identity, 80.7%similarity) WP_002191947.1 (69.8% identity, 80.7% similarity) Cry35Ac2(23.7% identity, 40.4% similarity) APG00851 222 386 APG00427 (88.4%identity, 93.1% similarity) Mtx 85, 90, 95, 96, 97, 98, 99 90, 95, 96,97, 98, 99 WP_018673409.1 (81.6% identity, 89.0% similarity)WP_001039209.1 (67.2% identity, 78.8% similarity) US_8829279_B2-61(56.3% identity, 69.3% similarity) US_8829279_B2-2 (55.6% identity,68.6% similarity) APG00862 223 WP_002167240.1 (27.4% identity, 40.1%similarity) Bin 30, 35, 40, 45, 50, 55, 60, 65, 45, 50, 55, 60, 65, 70,75, 80, 70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98,99 WP_016093722.1 (27.3% identity, 42.1% similarity) WP_002016877.1(25.7% identity, 38.9% similarity) Cry35Aa2 (19.4% identity, 31.1%similarity) APG00898 224 387 APG00201 (74.3% identity, 85.2% similarity)Mtx 70, 75, 80, 85, 90, 95, 96, 97, 85, 90, 95, 96, 97, 98, 99 98, 99APG00036 (70.4% identity, 82.2% similarity) APG00006 (69.6% identity,82.7% similarity) APG00137 (69.2% identity, 80.0% similarity)WP_000963933.1 (69.0% identity, 81.5% similarity) US20130227743A1_100(68.8% identity, 80.6% similarity) APG00022 (68.2% identity, 79.2%similarity) APG00067 (67.9% identity, 80.7% similarity) APG00155 (67.1%identity, 79.3% similarity) APG00107 (64.8% identity, 78.8% similarity)US20130227743A1_60 (41.5% identity, 47.4% similarity) WP_026324166.1(25.7% identity, 39.5% similarity) APG00907 225 388 AGA40061.1 (59.2%identity, 73.5% similarity) Mtx 60, 65, 70, 75, 80, 85, 90, 95, 75, 80,85, 90, 95, 96, 97, 98, 96, 97, 98, 99 99 APG00112 (57.7% identity,69.5% similarity) AGA40062.1 (54.2% identity, 69.3% similarity) Cry64Aa1(38.3% identity, 52.6% similarity) APG00913 226 389 APG00118 (90.9%identity, 92.7% similarity) Bin 80, 85, 90, 95, 96, 97, 98, 99 85, 90,95, 96, 97, 98, 99 APG00398 (85.9% identity, 88.4% similarity) APG00223(85.1% identity, 90.0% similarity) APG00454 (84.5% identity, 89.8%similarity) WP_002187944.1 (77.8% identity, 81.8% similarity)US20130227743A1_10 (75.8% identity, 81.6% similarity) WP_001258160.1(75.6% identity, 81.4% similarity) APG00065 (60.4% identity, 72.8%similarity) Cry35Ac2 (18.9% identity, 33.3% similarity) APG00925 227 390APG00369 (79.6% identity, 87.1% similarity) Bin 65, 70, 75, 80, 85, 90,95, 96, 75, 80, 85, 90, 95, 96, 97, 98, 97, 98, 99 99 APG00262 (73.7%identity, 82.5% similarity) APG00282 (69.9% identity, 75.6% similarity)WP_016107065.1 (63.4% identity, 72.0% similarity) APG00065 (63.2%identity, 71.8% similarity) WP_002191947.1 (61.7% identity, 71.3%similarity) US20130227743A1_148 (61.7% identity, 70.2% similarity)APG00398 (60.2% identity, 71.9% similarity) Cry49Aa1 (19.5% identity,34.2% similarity) APG00568 391 392 APG00356 (97.6% identity, 99.2%similarity) Bin 95, 96, 97, 98, 99 98, 99 APG00287 (96.2% identity,98.1% similarity) APG00231 (95.7% identity, 95.7% similarity) APG00377(95.4% identity, 98.7% similarity) WP_050845516.1 (93.8% identity, 97.9%similarity) AEX56523.1 (93.3% identity, 97.6% similarity) APG00035(89.5% identity, 94.1% similarity) WP_000143307.1 (86.9% identity, 92.5%similarity) WP_000143308.1 (84.5% identity, 91.4% similarity)US_2013_0227743_A1-6 (78.6% identity, 84.2% similarity) APG00011 (77.7%identity, 85.5% similarity) APG00716 393 394 APG00376 (98.4% identity,98.5% similarity) Bin 75, 80, 85, 90, 95, 96, 97, 98, 99 85, 90, 95, 96,97, 98, 99 APG00221 (92.7% identity, 95.1% similarity) APG00237 (85.8%identity, 90.1% similarity) APG00353 (79.3% identity, 86.0% similarity)WP_000839920.1 (70.3% identity, 81.4% similarity) WP_002166959.1 (70.2%identity, 79.9% similarity) WP_002191947.1 (70.0% identity, 79.9%similarity) US_2013_0227743_A1-146 (67.2% identity, 77.0% similarity)APG00065 (66.2% identity, 76.5% similarity) APG00736 395 396 APG00647(72.9% identity, 79.1% similarity) Cyt 70, 75, 80, 85, 90, 95, 96, 97,98, 99 80, 85, 90, 95, 96, 97, 98, 99 APG00290 (71.8% identity, 79.5%similarity) APG00698 (70.8% identity, 78.4% similarity) APG00422 (69.9%identity, 75.8% similarity) WP_058323095.1 (69.9% identity, 75.8%similarity) APG00930 397 APG01245 (97.0% identity, 98.3% similarity) Mtx80, 85, 90, 95, 96, 97, 98, 99 90, 95, 96, 97, 98, 99 APG00107 (93.9%identity, 96.6% similarity) APG00155 (76.4% identity, 86.5% similarity)WP_000963933.1 (76.4% identity, 87.2% similarity) US_2013_0227743_A1-100(76.0% identity, 86.5% similarity) APG00201 (74.3% identity, 82.9%similarity) APG00006 (73.9% identity, 82.7% similarity) APG00067 (70.8%identity, 83.9% similarity) APG00036 (70.8% identity, 80.4% similarity)APG00022 (69.4% identity, 80.7% similarity) APG01245 398 APG00930 (97.0%identity, 98.3% similarity) Mtx 80, 85, 90, 95, 96, 97, 98, 99 90, 95,96, 97, 98, 99 APG00107 (93.2% identity, 96.6% similarity)US_2013_0227743_A1-100 (77.4% identity, 86.9% similarity) WP_000963933.1(76.7% identity, 86.8% similarity) APG00155 (75.7% identity, 85.8%similarity) APG00201 (74.3% identity, 81.9% similarity) APG00006 (74.1%identity, 83.9% similarity) APG00067 (71.1% identity, 83.6% similarity)APG00036 (70.0% identity, 80.3% similarity) APG00022 (69.4% identity,80.4% similarity)

i. Classes of Pesticidal Proteins

The pesticidal proteins provided herein and the nucleotide sequencesencoding them are useful in methods for impacting pests. That is, thecompositions and methods of the invention find use in agriculture forcontrolling or killing pests, including pests of many crop plants. Thepesticidal proteins provided herein are toxin proteins from bacteria andexhibit activity against certain pests. The pesticidal proteins are fromseveral classes of toxins including Cry, Cyt, BIN, and Mtx toxins. See,for example, Table 1 for the specific protein classifications of thevarious SEQ ID NOS provided herein. In addition, reference is madethroughout this disclosure to Pfam database entries. The Pfam databaseis a database of protein families, each represented by multiple sequencealignments and a profile hidden Markov model. Finn et al. (2014) Nucl.Acid Res. Database Issue 42:D222-D230.

Bacillus thuringiensis (Bt) is a gram-positive bacterium that producesinsecticidal proteins as crystal inclusions during its sporulation phaseof growth. The proteinaceous inclusions of Bacillus thuringiensis (Bt)are called crystal proteins or 6-endotoxins (or Cry proteins), which aretoxic to members of the class Insecta and other invertebrates.Similarly, Cyt proteins are parasporal inclusion proteins from Bt thatexhibits hemolytic (cytolytic) activity or has obvious sequencesimilarity to a known Cyt protein. These toxins are highly specific totheir target organism, and are innocuous to humans, vertebrates, andplants.

The structure of the Cry toxins reveals five conserved amino acidblocks, concentrated mainly in the center of the domain or at thejunction between the domains. The Cry toxin consists of three domains,each with a specific function. Domain I is a seven α-helix bundle inwhich a central helix is completely surrounded by six outer helices.This domain is implicated in channel formation in the membrane. DomainII appears as a triangular column of three anti-parallel β-sheets, whichare similar to antigen-binding regions of immunoglobulins. Domain IIIcontains anti-parallel β-strands in a β sandwich form. The N-terminalpart of the toxin protein is responsible for its toxicity andspecificity and contains five conserved regions. The C-terminal part isusually highly conserved and probably responsible for crystal formation.See, for example, U.S. Pat. No. 8,878,007.

Strains of B. thuringiensis show a wide range of specificity againstdifferent insect orders (Lepidoptera, Diptera, Coleoptera, Hymenoptera,Homoptera, Phthiraptera or Mallophaga, and Acari) and otherinvertebrates (Nemathelminthes, Platyhelminthes, and Sarocomastebrates).The Cry proteins have been classified into groups based on toxicity tovarious insect and invertebrate groups. Generally, Cry I demonstratestoxicity to lepidopterans, Cry II to lepidopterans and dipterans, CryIIIto coleopterans, Cry IV to dipterans, and Cry V and Cry VI to nematodes.New Cry proteins can be identified and assigned to a Cry group based onamino acid identity. See, for example, Bravo, A. (1997) J. of Bacteriol.179:2793-2801; Bravo et al. (2013) Microb. Biotechnol. 6:17-26, hereinincorporated by reference.

Over 750 different cry gene sequences have been classified into 73groups (Cry1-Cry73), with new members of this gene family continuing tobe discovered (Crickmore et al. (2014) www.btnomenclature.info/). Thecry gene family consists of several phylogentically non-related proteinfamilies that may have different modes of action: the family ofthree-domain Cry toxins, the family of mosquitocidal Cry toxins, thefamily of the binary-like toxins, and the Cyt family of toxins (Bravo etal., 2005). Some Bt strains produce additional insecticidal toxins, theVIP toxins. See, also, Cohen et al. (2011) J. Mol. Biol. 413:4-814;Crickmore et al. (2014) Bacillus thuringiensis toxin nomenclature, foundon the world wide web at lifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/;Crickmore et al. (1988) Microbiol. Mol. Biol. Rev. 62: 807-813; Gill etal. (1992) Ann. Rev. Entomol. 37: 807-636; Goldbert et al. (1997) Appl.Environ. Microbiol. 63:2716-2712; Knowles et al. (1992) Proc. R. Soc.Ser. B. 248: 1-7; Koni et al. (1994) Microbiology 140: 1869-1880; Lailaket al. (2013) Biochem. Biophys. Res. Commun. 435: 216-221; Lopez-Diaz etal. (2013) Environ. Microbiol. 15: 3030-3039; Perez et al. (2007) Cell.Microbiol. 9: 2931-2937; Promdonkoy et al. (2003) Biochem. J. 374:255-259; Rigden (2009) FEBS Lett. 583: 1555-1560; Schnepf et al. (1998)Microbiol. Mol. Biol. Rev. 62: 775-806; Soberon et al. (2013) Peptides41: 87-93; Thiery et al. (1998) J. Am. Mosq. Control Assoc. 14: 472-476;Thomas et al. (1983) FEBS Lett. 154: 362-368; Wirth et al. (1997) Proc.Natl. Acad. Sci. U.S.A. 94: 10536-10540; Wirth et al (2005) Appl.Environ. Microbiol. 71: 185-189; and, Zhang et al. (2006) Biosci.Biotechnol. Biochem. 70: 2199-2204; each of which is herein incorporatedby reference in their entirety.

Cyt designates a parasporal crystal inclusion protein from Bacillusthuringiensis with cytolytic activity, or a protein with sequencesimilarity to a known Cyt protein. (Crickmore et al. (1998) Microbial.Mol. Biol. Rev. 62: 807-813). The gene is denoted by cyt. These proteinsare different in structure and activity from Cry proteins (Gill et al.(1992) Annu. Rev. Entomol. 37: 615-636). The Cyt toxins were firstdiscovered in B. thuringiensis subspecies israelensis (Goldberg et al.(1977) Mosq. News. 37: 355-358). There are 3 Cyt toxin familiesincluding 11 holotype toxins in the current nomenclature (Crickmore etal. (2014) Bacillus thuringiensis toxin nomenclature found on the worldwide web atlifesci.sussex.ac.uk/home/Neil_Crickmore/Bt/). The majorityof the B. thuringiensis isolates with cyt genes show activity againstdipteran insects (particularly mosquitoes and black flies), but thereare also cyt genes that have been described in B. thuringiensis strainstargeting lepidopteran or coleopteran insects (Guerchicoff et al. (1997)Appl. Environ. Microbiol. 63: 2716-2721).

The structure of Cyt2A, solved by X-ray crystallography, shows a singledomain where two outer layers of α-helix wrap around a mixed β-sheet.Further available crystal structures of Cyt toxins support a conservedα-β structural model with two α-helix hairpins flanking a β-sheet corecontaining seven to eight β-strands. (Cohen et al. (2011) J. Mol. Biol.413: 80 4-814) Mutagenic studies identified β-sheet residues as criticalfor toxicity, while mutations in the helical domains did not affecttoxicity (Adang et al.; Diversity of Bacillus thuringiensis CrystalToxins and Mechanism of Action. In: T. S. Dhadialla and S. S. Gill, eds,Advances in Insect Physiology, Vol. 47, Oxford: Academic Press, 2014,pp. 39-87.) The representative domain of the Cyt toxin is a δ-endotoxin,Bac_thur_toxin (Pfam PF01338).

There are multiple proposed models for the mode of action of Cyt toxins,and it is still an area of active investigation. Some Cyt proteins(Cyt1A) have been shown to require the presence of accessory proteinsfor crystallization. Cyt1 A and Cyt2A protoxins are processed bydigestive proteases at the same sites in the N- and C-termini to astable toxin core. Cyt toxins then interact with non-saturated membranelipids, such as phosphatidylcholine, phosphatidylethanolamine, andsphingomyelin. For Cyt toxins, pore-formation and detergent-likemembrane disruption have been proposed as non-exclusive mechanisms; andit is generally accepted that both may occur depending on toxinconcentration, with lower concentrations favoring oligomeric pores andhigher concentrations leading to membrane breaks. (Butko (2003) Appl.Environ. Microbiol. 69: 2415-2422) In the pore-formation model, the Cyttoxin binds to the cell membrane, inducing the formation ofcation-selective channels in the membrane vesicles leading tocolloid-osmotic lysis of the cell. (Knowles et al. (1989) FEBS Lett.244: 259-262; Knowles et al. (1992) Proc. R. Soc. Ser. B. 248: 1-7 andPromdonkoy et al. (2003) Biochem. 1 374: 255-259). In the detergentmodel, there is a nonspecific aggregation of the toxin on the surface ofthe lipid bilayer leading to membrane disassembly and cell death. (Butko(2003) supra; Manceva et al. (2005) Biochem. 44: 589-597).

Multiple studies have shown synergistic activity between Cyt toxins andother B. thuringiensis toxins, particularly the Cry, Bin, and Mtxtoxins. This synergism has even been shown to overcome an insect'sresistance to the other toxin. (Wirth 1997, Wirth 2005, Thiery 1998,Zhang 2006) The Cyt synergistic effect for Cry toxins is proposed toinvolve Cyt1 A binding to domain II of Cry toxins in solution or on themembrane plane to promote formation of a Cry toxin pre-pore oligomer.Formation of this oligomer is independent of the Cyt oligomerization,binding or insertion. (Lailak 2013, Perez 2007, Lopez-Diaz 2013)

A number of pesticidal proteins unrelated to the Cry proteins areproduced by some strains of B. thuringiensis and B. cereus duringvegetative growth (Estruch et al. (1996) Proc Natl Acad Sci USA93:5389-5394; Warren et al. (1994) WO 94/21795). These vegetativeinsecticidal proteins, or Vips, do not form parasporal crystal proteinsand are apparently secreted from the cell. The Vips are presentlyexcluded from the Cry protein nomenclature because they are notcrystal-forming proteins. The term VIP is a misnomer in the sense thatsome B. thuringiensis Cry proteins are also produced during vegetativegrowth as well as during the stationary and sporulation phases, mostnotably Cry3Aa. The location of the Vip genes in the B. thuringiensisgenome has been reported to reside on large plasmids that also encodecry genes (Mesrati et al. (2005) FEMS Microbiol. Lett. 244(2):353-8). Aweb-site for the nomenclature of Bt toxins can be found on the worldwide web at lifesci.sussex.ac.uk with the path“/home/Neil_Crickmore/Bt/” and at: “btnomenclature.info/”. See also,Schnepf et al. (1998) Microbiol. Mol. Biol. Rev. 62(3):775-806. Suchreferences are herein incorporated by reference.

To date four categories of Vips have been identified. Some Vip genesform binary two-component protein complexes; an “A” component is usuallythe “active” portion, and a “B” component is usually the “binding”portion. (Pfam pfam.xfam.org/family/PF03495). The Vip1 and Vip4 proteinsgenerally contain binary toxin B protein domains. Vip2 proteinsgenerally contain binary toxin A protein domains.

The Vip1 and Vip2 proteins are the two components of a binary toxin thatexhibits toxicity to coleopterans. Vip1Aa1 and Vip2Aa1 are very activeagainst corn rootworms, particularly Diabrotica virgifera and Diabroticalongicornis (Han et al. (1999) Nat. Struct. Biol. 6:932-936; Warren GW(1997) “Vegetative insecticidal proteins: novel proteins for control ofcorn pests” In: Carozzi NB, Koziel M (eds) Advances in insect control,the role of transgenic plants; Taylor & Francis Ltd, London, pp 109-21).The membrane-binding 95 kDa Vip1 multimer provides a pathway for the 52kDa Vip2 ADP-ribosylase to enter the cytoplasm of target western cornrootworm cells (Warren (1997) supra). The NAD-dependentADP-ribosyltransferase Vip2 likely modifies monomeric actin at Arg177 toblock polymerization, leading to loss of the actin cytoskeleton andeventual cell death due to the rapid subunit ex-change within actinfilaments in vivo (Carlier M. F. (1990) Adv. Biophys. 26:51-73).

Like Cry toxins, activated Vip3A toxins are pore-forming proteinscapable of making stable ion channels in the membrane (Lee et al. (2003)Appl. Environ. Microbiol. 69:4648-4657). Vip3 proteins are activeagainst several major lepidopteran pests (Rang et al. (2005) Appl.Environ. Microbiol. 71(10):6276-6281; Bhalla et al. (2005) FEMSMicrobiol. Lett. 243:467-472; Estruch et al. (1998) WO 9844137; Estruchet al. (1996) Proc Natl Acad Sci USA 93:5389-5394; Selvapandiyan et al.(2001) Appl. Environ Microbiol. 67:5855-5858; Yu et al. (1997) Appl.Environ Microbiol. 63:532-536). Vip3A is active against Agrotis ipsilon,Spodoptera frupperda, Spodoptera exigua, Heliothis virescens, andHelicoverpa zea (Warren et al. (1996) WO 96/10083; Estruch et al. (1996)Proc Natl Acad Sci USA 93:5389-5394). Like Cry toxins, Vip3A proteinsmust be activated by proteases prior to recognition at the surface ofthe midgut epithelium of specific membrane proteins different from thoserecognized by Cry toxins.

The MTX family of toxin proteins is characterized by the presence of aconserved domain, ETX_MTX2 (pfam 03318). Members of this family sharesequence homology with the mosquitocidal toxins Mtx2 and Mtx3 fromBacillus sphaericus, as well as with the epsilon toxin ETX fromClostridium perfringens (Cole et al. (2004) Nat. Struct. Mol. Biol. 11:797-8; Thanabalu et al. (1996) Gene 170:85-9). The MTX-like proteins arestructurally distinct from the three-domain Cry toxins, as they have anelongated and predominately β-sheet-based structure. However, similar tothe three-domain toxins, the MTX-like proteins are thought to form poresin the membranes of target cells (Adang et al. (2014) supra). Unlike thethree-domain Cry proteins, the MTX-like proteins are much smaller inlength, ranging from 267 amino acids (Cry23) to 340 amino acids(Cry15A).

To date, only 15 proteins belonging to the family of MTX-like toxinshave been assigned Cry names, making this a relatively small classcompared to the three-domain Cry family (Crickmore et al. (2014) supra;Adang et al. (2014) supra). The members of the MTX-like toxin familyinclude Cry15, Cry23, Cry33, Cry38, Cry45, Cry46, Cry51, Cry60A, Cry60B,and Cry64. This family exhibits a range of insecticidal activity,including activity against insect pests of the Lepidopteran andColeopteran orders. Some members of this family may form binarypartnerships with other proteins, which may or may not be required forinsecticidal activity.

Cry15 is a 34 kDA protein that was identified in Bacillus thuringiensisserovar thompsoni HD542; it occurs naturally in a crystal together withan unrelated protein of approximately 40 kDa. The gene encoding Cry15and its partner protein are arranged together in an operon. Cry15 alonehas been shown to have activity against lepidopteran insect pestsincluding Manduca sexta, Cydia pomonella, and Pieris rapae, with thepresence of the 40 kDA protein having been shown to increase activity ofCry15 only against C. pomonella (Brown K. and Whiteley H. (1992) J.Bacteriol. 174:549-557; Naimov et al. (2008) Appl. Environ. Microbiol.74:7145-7151). Further studies are needed to elucidate the function ofthe partner protein of Cry15. Similarly, Cry23 is a 29 kDA protein thathas been shown to have activity against the coleopteran pests Triboliumcastaneum and Popillia japonica together with its partner protein Cry37(Donovan et al. (2000) U.S. Pat. No. 6,063,756).

New members of the MTX-like family are continuing to be identified. AnETX_MTX toxin gene was recently identified in the genome of Bacillusthuringiensis serovar tolworthi strain Na205-3. This strain was found tobe toxic against the lepidpoteran pest Helicoverpa armigera, and it alsocontained homologs of Cry1, Cry11, Vip1, Vip2, and Vip3 (Palma et al.(2014) Genome Announc. 2(2): e00187-14. Published online Mar. 13, 2014at doi: 10.1128/genomeA.00187-14; PMCID: PMC3953196). Because theMTX-like proteins have a unique domain structure relative to thethree-domain Cry proteins, they are believed to possess a unique mode ofaction, thereby making them a valuable tool in insect control and thefight against insect resistance.

Bacterial cells produce large numbers of toxins with diverse specificityagainst host and non-host organisms. Large families of binary toxinshave been identified in numerous bacterial families, including toxinsthat have activity against insect pests. (Poopathi and Abidha (2010) J.Physiol. Path. 1(3): 22-38). Lysinibacillus sphaericus (Ls), formerlyBacillus sphaericus, (Ahmed et al. (2007) Int. J Syst. Evol. Microbiol.57:1117-1125) is well-known as an insect biocontrol strain. Ls producesseveral insecticidal proteins, including the highly potent binarycomplex BinA/BinB. This binary complex forms a parasporal crystal in Lscells and has strong and specific activity against dipteran insects,specifically mosquitos. In some areas, insect resistance to existing Lsmosquitocidal strains has been reported. The discovery of new binarytoxins with different target specificity or the ability to overcomeinsect resistance is of significant interest.

The Ls binary insecticidal protein complex contains two majorpolypeptides, a 42 kDa polypeptide and a 51 kDa polypepdide, designatedBinA and BinB, respectively (Ahmed et al. (2007) supra). The twopolypeptides act synergistically to confer toxicity to their targets.Mode of action involves binding of the proteins to receptors in thelarval midgut. In some cases, the proteins are modified by proteasedigestion in the larval gut to produce activated forms. The BinBcomponent is thought to be involved in binding, while the BinA componentconfers toxicity (Nielsen-LeRoux et al. (2001) Appl. Environ. Microbiol.67(11):5049-5054). When cloned and expressed separately, the BinAcomponent is toxic to mosquito larvae, while the BinB component is not.However, co-administration of the proteins markedly increases toxicity(Nielsen-LeRoux et al. (2001) supra).

A small number of Bin protein homologs have been described frombacterial sources. Priest et al. (1997) Appl. Environ. Microbiol.63(4):1195-1198 describe a hybridization effort to identify new Lsstrains, although most of the genes they identified encoded proteinsidentical to the known BinA/BinB proteins. The BinA protein contains adefined conserved domain known as the Toxin 10 superfamily domain. Thistoxin domain was originally defined by its presence in BinA and BinB.The two proteins both have the domain, although the sequence similaritybetween BinA and BinB is limited in this region (<40%). The Cry49Aaprotein, which also has insecticidal activity, also has this domain(described below).

The Cry48Aa/Cry49Aa binary toxin of Ls has the ability to kill Culexquinquefasciatus mosquito larvae. These proteins are in a proteinstructural class that has some similarity to the Cry protein complex ofBacillus thuringiensis (Bt), a well-known insecticidal protein family.The Cry34/Cry35 binary toxin of Bt is also known to kill insects,including Western corn rootworm, a significant pest of corn. Cry34, ofwhich several variants have been identified, is a small (14 kDa)polypeptide, while Cry35 (also encoded by several variants) is a 44 kDapolypeptide. These proteins have some sequence homology with theBinA/BinB protein group and are thought to be evolutionarily related(Ellis et al. (2002) Appl. Environ. Microbiol. 68(3):1137-1145).

Provided herein are pesticidal proteins from these classes of toxins.The pesticidal proteins are classified by their structure, homology toknown toxins and/or their pesticidal specificity.

ii. Variants and Fragments of Pesticidal Proteins and PolynucleotidesEncoding the Same

Pesticidal proteins or polypeptides of the invention include those setforth in SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51,52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69,70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87,88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118,119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132,133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146,147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160,161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174,175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216,217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230,231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258,259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272,273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286,287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300,301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314,315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328,329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342,343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356,357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, or 398and fragments and variants thereof. By “pesticidal toxin” or “pesticidalprotein” or “pesticidal polypeptide” is intended a toxin or protein orpolypeptide that has activity against one or more pests, including,insects, fungi, nematodes, and the like such that the pest is killed orcontrolled.

An “isolated” or “purified” polypeptide or protein, or biologicallyactive portion thereof, is substantially or essentially free fromcomponents that normally accompany or interact with the polypeptide orprotein as found in its naturally occurring environment. Thus, anisolated or purified polypeptide or protein is substantially free ofother cellular material, or culture medium when produced by recombinanttechniques, or substantially free of chemical precursors or otherchemicals when chemically synthesized. A protein that is substantiallyfree of cellular material includes preparations of protein having lessthan about 30%, 20%, 10%, 5%, or 1% (by dry weight) of contaminatingprotein. When the protein of the invention or biologically activeportion thereof is recombinantly produced, optimally culture mediumrepresents less than about 30%, 20%, 10%, 5%, or 1% (by dry weight) ofchemical precursors or non-protein-of-interest chemicals.

The term “fragment” refers to a portion of a polypeptide sequence of theinvention. “Fragments” or “biologically active portions” includepolypeptides comprising a sufficient number of contiguous amino acidresidues to retain the biological activity, i.e., have pesticidalactivity. Fragments of the pesticidal proteins include those that areshorter than the full-length sequences, either due to the use of analternate downstream start site, or due to processing that produces ashorter protein having pesticidal activity. Processing may occur in theorganism the protein is expressed in, or in the pest after ingestion ofthe protein. Examples of fragments of the proteins can be found inTable 1. A biologically active portion of a pesticidal protein can be apolypeptide that is, for example, 10, 25, 50, 100, 150, 200, 250 or moreamino acids in length of any one of SEQ ID NO:1, 2, 3, 4, 5, 6, 7, 8, 9,10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27,28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45,46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63,64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99,100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113,114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141,142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183,184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197,198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211,212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225,226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239,240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267,268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281,282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295,296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323,324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337,338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365,366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379,380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393,394, 395, 396, 397, or 398. Such biologically active portions can beprepared by recombinant techniques and evaluated for pesticidalactivity. As used here, a fragment comprises at least 8 contiguous aminoacids of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15,16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51,52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69,70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87,88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118,119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132,133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145, 146,147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160,161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174,175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202,203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216,217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230,231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258,259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272,273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286,287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300,301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313, 314,315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327, 328,329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342,343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356,357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, or 398.

Bacterial genes, including those encoding the pesticidal proteinsdisclosed herein, quite often possess multiple methionine initiationcodons in proximity to the start of the open reading frame. Often,translation initiation at one or more of these start codons will lead togeneration of a functional protein. These start codons can include ATGcodons. However, bacteria such as Bacillus sp. also recognize the codonGTG as a start codon, and proteins that initiate translation at GTGcodons contain a methionine at the first amino acid. On rare occasions,translation in bacterial systems can initiate at a TTG codon, though inthis event the TTG encodes a methionine. Furthermore, it is not oftendetermined a priori which of these codons are used naturally in thebacterium. Thus, it is understood that use of one of the alternatemethionine codons may also lead to generation of pesticidal proteins.These pesticidal proteins are encompassed in the present invention andmay be used in the methods disclosed herein. It will be understood that,when expressed in plants, it will be necessary to alter the alternatestart codon to ATG for proper translation.

In various embodiments the pesticidal proteins provided herein includeamino acid sequences deduced from the full-length nucleotide sequencesand amino acid sequences that are shorter than the full-length sequencesdue to the use of an alternate downstream start site. Thus, thenucleotide sequence of the invention and/or vectors, host cells, andplants comprising the nucleotide sequence of the invention (and methodsof making and using the nucleotide sequence of the invention) maycomprise a nucleotide sequence encoding an alternate start site.

It is recognized that modifications may be made to the pesticidalpolypeptides provided herein creating variant proteins. Changes designedby man may be introduced through the application of site-directedmutagenesis techniques. Alternatively, native, as yet-unknown or as yetunidentified polynucleotides and/or polypeptides structurally and/orfunctionally-related to the sequences disclosed herein may also beidentified that fall within the scope of the present invention.Conservative amino acid substitutions may be made in nonconservedregions that do not alter the function of the pesticidal proteins.Alternatively, modifications may be made that improve the activity ofthe toxin. Modification of Cry toxins by domain III swapping hasresulted in some cases in hybrid toxins with improved toxicities againstcertain insect species. Thus, domain III swapping could be an effectivestrategy to improve toxicity of Cry toxins or to create novel hybridtoxins with toxicity against pests that show no susceptibility to theparental Cry toxins. Site-directed mutagenesis of domain II loopsequences may result in new toxins with increased insecticidal activity.Domain II loop regions are key binding regions of initial Cry toxinsthat are suitable targets for the mutagenesis and selection of Crytoxins with improved insecticidal properties. Domain I of the Cry toxinmay be modified to introduce protease cleavage sites to improve activityagainst certain pests. Strategies for shuffling the three differentdomains among large numbers of cry genes and high throughput bioassayscreening methods may provide novel Cry toxins with improved or noveltoxicities.

As indicated, fragments and variants of the polypeptides disclosedherein will retain pesticidal activity. Pesticidal activity comprisesthe ability of the composition to achieve an observable effectdiminishing the occurrence or an activity of the target pest, includingfor example, bringing about death of at least one pest, or a noticeablereduction in pest growth, feeding, or normal physiological development.Such decreases in numbers, pest growth, feeding or normal developmentcan comprise any statistically significant decrease, including, forexample a decrease of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%,50%, 55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% or greater. It is recognizedthat the pesticidal activity may be different or improved relative tothe activity of the native protein, or it may be unchanged, so long aspesticidal activity is retained. Methods for measuring pesticidalactivity are well known in the art. See, for example, Czapla and Lang(1990) J. Econ. Entomol. 83:2480-2485; Andrews et al. (1988) Biochem. J.252:199-206; Marrone et al. (1985) J. of Economic Entomology 78:290-293;and U.S. Pat. No. 5,743,477, all of which are herein incorporated byreference in their entirety.

Polypeptide variants of this disclosure include polypeptides having anamino acid sequence that is at least about 60%, about 65%, about 70%,about 75%, about 80%, about 85%, about 86%, about 87%, about 88%, about89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%,about 96%, about 97%, about 98% or about 99% identical to the amino acidsequence of any of SEQ ID NOs: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129,130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157,158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171,172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185,186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199,200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213,214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255,256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269,270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297,298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311,312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339,340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353,354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367,368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381,382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395,396, 397, or 398 and retain pesticidal activity. Note, Table 1 providesnon-limiting examples of variant polypeptides (and polynucleotideencoding the same) for each of SEQ ID NOS: 1-398. A biologically activevariant of a pesticidal polypeptide of the invention may differ by asfew as about 1-15 amino acid residues, as few as about 1-10, such asabout 6-10, as few as 5, as few as 4, as few as 3, as few as 2, or asfew as 1 amino acid residue. In specific embodiments, the polypeptidescan comprise an N′-terminal or a C′-terminal truncation, which cancomprise at least a deletion of 10, 15, 20, 25, 30, 35, 40, 45, 50 aminoacids or more from either the N′ or C′ terminal end of the polypeptide.

Table 2 provides protein domains found in SEQ ID NOs: 1-398 based onPFAM data. Both the domain description and the positions within a givenSEQ ID NO are provided in Table 2. In specific embodiments, the activevariant comprising any one of SEQ ID NOs: 1-398 can comprise at least70%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequenceidentity to any one of SEQ ID NOs: 1-398 and further comprises at leastone of the conserved domain set forth in Table 2. For example, in oneembodiment, the active variant will comprise at least 70%, 75%, 76%,77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%,91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQID NO:1, and further comprises the native amino acids at positions54-304.

TABLE 2 Summary of PFAM domains in each of SEQ ID NOs: 1-398 DomainModification Domain Position APG ID Seq ID Type PFAM Domain DescriptionStart Stop APG00056 Seq ID 1 PF03945 Endotoxin N 54 304 PF00555Endotoxin M 311 500 PF03944 Endotoxin C 510 646 APG00056 Seq ID 2 3′Truncation PF03945 Endotoxin N 54 304 modified PF00555 Endotoxin M 311500 PF03944 Endotoxin C 510 645 APG00058 Seq ID 3 PF03945 Endotoxin N 68320 PF00555 Endotoxin M 327 513 PF03944 Endotoxin C 523 658 APG00058 SeqID 4 3′ Truncation PF03945 Endotoxin N 68 320 modified PF00555 EndotoxinM 327 513 PF03944 Endotoxin C 523 657 APG00064 Seq ID 5 PF03318 ETX MTX294 329 APG00064 Seq ID 6 Signal peptide PF03318 ETX MTX2 67 302 modifiedremoved APG00067 Seq ID 7 PF03318 ETX MTX2 29 252 APG00071 Seq ID 8PF12495 Vip3A N 12 188 APG00071 Seq ID 9 Alternate start PF12495 Vip3A N9 185 modified APG00073 Seq ID 10 PF03945 Endotoxin N 5 244 PF03944Endotoxin C 478 612 APG00073 Seq ID 11 Alternate start PF03945 EndotoxinN 5 244 modified and 3′ PF03944 Endotoxin C 478 611 Truncation APG00073Seq ID 12 Alternate start PF03945 Endotoxin N 5 244 modified PF03944Endotoxin C 478 612 APG00073 Seq ID 13 3′ Truncation PF03945 Endotoxin N5 244 modified PF03944 Endotoxin C 478 611 APG00074 Seq ID 14 PF12495Vip3A N 16 191 APG00084 Seq ID 15 PF03945 Endotoxin N 76 300 PF00555Endotoxin M 305 529 PF03944 Endotoxin C 539 673 APG00084 Seq ID 16 3′Truncation PF03945 Endotoxin N 76 300 modified PF00555 Endotoxin M 305529 PF03944 Endotoxin C 539 672 APG00105 Seq ID 17 PF03945 Endotoxin N95 330 PF00555 Endotoxin M 335 542 PF03944 Endotoxin C 552 694 APG00105Seq ID 18 Alternate start PF03945 Endotoxin N 64 299 modified PF00555Endotoxin M 304 511 PF03944 Endotoxin C 521 663 APG00105 Seq ID 19Alternate start PF03945 Endotoxin N 64 299 modified and 3′ PF00555Endotoxin M 304 511 Truncation PF03944 Endotoxin C 521 662 APG00105 SeqID 20 3′ Truncation PF03945 Endotoxin N 95 330 modified PF00555Endotoxin M 335 542 PF03944 Endotoxin C 552 693 APG00107 Seq ID 21PF03318 ETX MTX2 29 250 APG00108 Seq ID 22 PF01338 Bac thur toxin 72 265APG00112 Seq ID 23 PF03318 ETX MTX2 28 267 APG00113 Seq ID 24 PF03945Endotoxin N 56 302 PF00030 Crystall 653 732 PF00030 Crystall 733 814PF14200 RicinB lectin 2 855 962 APG00116 Seq ID 25 PF03945 Endotoxin N72 294 PF00555 Endotoxin M 299 502 PF03944 Endotoxin C 512 645 APG00116Seq ID 26 3′ Truncation PF03945 Endotoxin N 72 294 modified PF00555Endotoxin M 299 502 PF03944 Endotoxin C 512 644 APG00117 Seq ID 27PF01338 Bac thur toxin 378 572 APG00118 Seq ID 28 PF05431 Toxin 10 237434 APG00121 Seq ID 29 PF01338 Bac thur toxin 52 272 APG00131 Seq ID 30PF12495 Vip3A N 10 187 APG00132 Seq ID 31 PF05431 Toxin 10 209 402APG00134 Seq ID 32 PF01338 Bac thur toxin 2 191 APG00137 Seq ID 33PF03318 ETX MTX2 21 256 APG00141 Seq ID 34 PF05431 Toxin 10 183 383APG00150 Seq ID 35 PF12495 Vip3A N 12 188 APG00152 Seq ID 36 PF03945Endotoxin N 70 293 PF00555 Endotoxin M 298 522 PF03944 Endotoxin C 532683 APG00152 Seq ID 37 3′ Truncation PF03945 Endotoxin N 70 293 modifiedPF00555 Endotoxin M 298 522 PF03944 Endotoxin C 532 682 APG00153 Seq ID38 PF03945 Endotoxin N 101 323 PF00555 Endotoxin M 328 531 PF03944Endotoxin C 541 670 APG00153 Seq ID 39 3′ Truncation PF03945 Endotoxin N101 323 modified PF00555 Endotoxin M 328 531 PF03944 Endotoxin C 541 669APG00155 Seq ID 40 PF03318 ETX MTX2 25 250 APG00164 Seq ID 41 PF03945Endotoxin N 56 279 PF00555 Endotoxin M 284 502 PF03944 Endotoxin C 512649 APG00164 Seq ID 42 3′ Truncation PF03945 Endotoxin N 56 279 modifiedPF00555 Endotoxin M 284 502 PF03944 Endotoxin C 512 648 APG00166 Seq ID43 PF12495 Vip3A N 16 188 APG00168 Seq ID 44 PF01338 Bac thur toxin 35257 APG00172 Seq ID 45 PF03318 ETX MTX2 36 238 APG00173 Seq ID 46PF12495 Vip3A N 16 188 APG00173 Seq ID 47 Alternate start PF12495 Vip3AN 14 186 modified APG00175 Seq ID 48 PF12495 Vip3A N 16 188 PF02018 CBM4 9 549 663 APG00175 Seq ID 49 Alternate start PF12495 Vip3A N 14 186modified PF02018 CBM 4 9 547 661 APG00176 Seq ID 50 PF03945 Endotoxin N58 280 PF00555 Endotoxin M 285 490 PF03944 Endotoxin C 500 637 APG00176Seq ID 51 3′ Truncation PF03945 Endotoxin N 58 280 modified PF00555Endotoxin M 285 490 PF03944 Endotoxin C 500 636 APG00177 Seq ID 52PF01338 Bac thur toxin 27 250 APG00177 Seq ID 53 3′ Truncation PF01338Bac thur toxin 27 224 modified APG00178 Seq ID 54 PF05431 Toxin 10 187379 APG00180 Seq ID 55 PF05791 Bacillus HBL 60 201 APG00186 Seq ID 56PF01338 Bac thur toxin 378 572 APG00188 Seq ID 57 PF03945 Endotoxin N 62323 PF00555 Endotoxin M 331 512 PF03944 Endotoxin C 527 669 APG00188 SeqID 58 3′ Truncation PF03945 Endotoxin N 62 323 modified PF00555Endotoxin M 331 512 PF03944 Endotoxin C 527 668 APG00189 Seq ID 59PF03945 Endotoxin N 38 274 PF00555 Endotoxin M 279 470 PF03944 EndotoxinC 480 617 APG00189 Seq ID 60 Alternate start PF03945 Endotoxin N 29 265modified PF00555 Endotoxin M 270 461 PF03944 Endotoxin C 471 608APG00189 Seq ID 61 3′ Truncation PF03945 Endotoxin N 38 274 modifiedPF00555 Endotoxin M 279 470 PF03944 Endotoxin C 480 616 APG00190 Seq ID62 PF03945 Endotoxin N 68 291 PF00555 Endotoxin M 296 522 PF03944Endotoxin C 532 676 APG00190 Seq ID 63 3′ Truncation PF03945 Endotoxin N68 291 modified PF00555 Endotoxin M 296 522 PF03944 Endotoxin C 532 675APG00192 Seq ID 64 PF05431 Toxin 10 159 358 APG00194 Seq ID 65 PF01338Bac thur toxin 27 223 APG00196 Seq ID 66 PF03945 Endotoxin N 58 302PF00030 Crystall 653 732 PF00030 Crystall 733 814 PF14200 RicinB lectin2 855 962 APG00198 Seq ID 67 PF01338 Bac thur toxin 2 190 APG00200 SeqID 68 PF01338 Bac thur toxin 282 482 APG00203 Seq ID 69 PF05431 Toxin 10189 382 APG00207 Seq ID 70 PF12495 Vip3A N 16 193 APG00209 Seq ID 71PF05791 Bacillus HBL 40 226 APG00211 Seq ID 73 PF14200 RicinB lectin 2 3102 PF05431 Toxin 10 156 353 APG00211 Seq ID 74 Alternate start PF14200RicinB lectin 2 2 98 modified PF05431 Toxin 10 152 349 APG00212 Seq ID75 PF05431 Toxin 10 216 409 APG00213 Seq ID 76 PF05431 Toxin 10 188 385APG00214 Seq ID 77 PF05791 Bacillus HBL 48 231 APG00215 Seq ID 78PF14200 RicinB lectin 2 2 96 PF05431 Toxin 10 148 343 APG00216 Seq ID 79PF00652 Ricin B lectin 38 165 PF05431 Toxin 10 175 287 APG00219 Seq ID80 PF03945 Endotoxin N 116 337 APG00221 Seq ID 81 PF05431 Toxin 10 192385 APG00223 Seq ID 82 PF05431 Toxin 10 235 432 APG00227 Seq ID 83PF03318 ETX MTX2 150 383 APG00229 Seq ID 84 PF05431 Toxin 10 210 403APG00230 Seq ID 85 PF00652 Ricin B lectin 36 167 PF05431 Toxin 10 177373 APG00231 Seq ID 86 PF14200 RicinB lectin 2 47 150 PF05431 Toxin 10156 353 APG00232 Seq ID 87 PF03945 Endotoxin N 110 310 PF14200 RicinBlectin 2 560 662 APG00233 Seq ID 88 PF03945 Endotoxin N 86 292 PF14200RicinB lectin 2 404 513 APG00235 Seq ID 89 PF03945 Endotoxin N 128 345PF14200 RicinB lectin 2 462 565 APG00237 Seq ID 90 PF05431 Toxin 10 200393 APG00239 Seq ID 91 PF03945 Endotoxin N 152 374 PF01473 CW binding 1432 450 PF01473 CW binding 1 453 472 APG00241 Seq ID 92 PF05791 BacillusHBL 44 225 APG00242 Seq ID 93 PF05431 Toxin 10 235 431 APG00243 Seq ID94 PF05431 Toxin 10 188 381 APG00248 Seq ID 95 PF05431 Toxin 10 224 416APG00249 Seq ID 96 PF05431 Toxin 10 219 413 APG00251 Seq ID 97 PF03945Endotoxin N 73 279 PF14200 RicinB lectin 2 391 500 APG00255 Seq ID 98PF03945 Endotoxin N 105 337 PF01473 CW binding 1 384 402 PF14200 RicinBlectin 2 484 587 APG00258 Seq ID 99 PF05791 Bacillus HBL 80 219 APG00261Seq ID 100 PF05431 Toxin 10 196 393 APG00262 Seq ID 101 PF05431 Toxin 10235 429 APG00264 Seq ID 103 PF05791 Bacillus HBL 49 229 APG00266 Seq ID105 PF03945 Endotoxin N 165 358 PF01473 CW binding 1 453 472 PF01473 CWbinding 1 474 489 APG00267 Seq ID 106 PF03945 Endotoxin N 122 321APG00271 Seq ID 108 PF05791 Bacillus HBL 32 209 APG00273 Seq ID 109PF12495 Vip3A N 16 188 PF02018 CBM 4 9 545 658 APG00274 Seq ID 110PF05791 Bacillus HBL 56 238 APG00275 Seq ID 111 PF03945 Endotoxin N 5129 APG00278 Seq ID 112 PF12495 Vip3A N 16 188 APG00278 Seq ID 113Alternate start PF12495 Vip3A N 14 186 modified APG00279 Seq ID 114PF05791 Bacillus HBL 128 239 APG00282 Seq ID 116 PF05431 Toxin 10 167362 APG00284 Seq ID 117 PF14200 RicinB lectin 2 47 150 PF05431 Toxin 10156 353 APG00285 Seq ID 118 PF03945 Endotoxin N 108 338 PF01473 CWbinding 1 407 421 PF01473 CW binding 1 456 470 PF01473 CW binding 1 573589 APG00286 Seq ID 119 PF05791 Bacillus HBL 37 217 APG00286 Seq ID 1203′ Truncation PF05791 Bacillus HBL 37 217 modified APG00287 Seq ID 121PF14200 RicinB lectin 2 53 150 PF05431 Toxin 10 156 353 APG00288 Seq ID122 PF03945 Endotoxin N 116 318 PF01473 CW binding 1 410 429 PF01473 CWbinding 1 431 445 APG00290 Seq ID 124 PF01338 Bac thur toxin 55 281APG00292 Seq ID 126 PF03945 Endotoxin N 95 304 PF05588 Botulinum 401 501HA-17 APG00294 Seq ID 127 PF05791 Bacillus HBL 46 229 APG00295 Seq ID128 PF05431 Toxin 10 224 420 APG00297 Seq ID 129 PF05791 Bacillus HBL 63243 APG00298 Seq ID 130 PF05791 Bacillus HBL 38 218 APG00301 Seq ID 131PF05431 Toxin 10 189 382 APG00302 Seq ID 132 PF03945 Endotoxin N 103 312PF14200 RicinB lectin 2 424 519 APG00305 Seq ID 133 PF05791 Bacillus HBL47 233 APG00307 Seq ID 134 PF05791 Bacillus HBL 43 228 APG00308 Seq ID135 PF05431 Toxin 10 203 397 APG00314 Seq ID 136 PF05431 Toxin 10 186380 APG00315 Seq ID 137 PF05431 Toxin 10 188 380 APG00320 Seq ID 140PF05431 Toxin 10 252 446 APG00340 Seq ID 141 PF14200 RicinB lectin 2 69170 PF05431 Toxin 10 176 374 APG00342 Seq ID 142 PF05791 Bacillus HBL 53205 APG00349 Seq ID 143 PF14200 RicinB lectin 2 1 97 PF05431 Toxin 10154 347 APG00353 Seq ID 144 PF05431 Toxin 10 189 382 APG00355 Seq ID 145PF03945 Endotoxin N 56 270 APG00355 Seq ID 146 3′ Truncation PF03945Endotoxin N 55 270 modified APG00356 Seq ID 147 PF14200 RicinB lectin 253 150 PF05431 Toxin 10 156 353 APG00368 Seq ID 148 PF14200 RicinBlectin 2 3 102 PF05431 Toxin 10 156 354 APG00369 Seq ID 149 PF05431Toxin 10 251 445 APG00372 Seq ID 150 PF03945 Endotoxin N 119 304 PF01473CW binding 1 420 439 PF01473 CW binding 1 441 455 APG00373 Seq ID 151PF00652 Ricin B lectin 38 165 PF05431 Toxin 10 176 372 APG00375 Seq ID152 PF03945 Endotoxin N 117 335 PF01473 CW binding 1 458 475 APG00376Seq ID 153 PF05431 Toxin 10 192 384 APG00377 Seq ID 154 PF14200 RicinBlectin 2 54 150 PF05431 Toxin 10 156 353 APG00379 Seq ID 155 PF14200RicinB lectin 2 47 148 PF05431 Toxin 10 156 353 APG00385 Seq ID 157PF05431 Toxin 10 203 396 APG00386 Seq ID 158 PF14200 RicinB lectin 2 42143 PF05431 Toxin 10 149 343 APG00388 Seq ID 159 PF01338 Bac thur toxin9 233 APG00391 Seq ID 160 PF03945 Endotoxin N 110 306 APG00392 Seq ID161 PF05431 Toxin 10 203 396 APG00395 Seq ID 162 PF03945 Endotoxin N 111332 PF01473 CW binding 1 433 449 PF01473 CW binding 1 461 477 APG00396Seq ID 163 PF03945 Endotoxin N 115 310 PF01473 CW binding 1 401 418PF01473 CW binding 1 420 439 PF01473 CW binding 1 523 538 APG00398 SeqID 165 PF05431 Toxin 10 278 475 APG00399 Seq ID 166 PF00041 fn3 430 523PF14200 RicinB lectin 2 1068 1192 PF05431 Toxin 10 1364 1558 APG00401Seq ID 168 PF03945 Endotoxin N 104 336 PF01473 CW binding 1 383 401PF14200 RicinB lectin 2 483 586 APG00404 Seq ID 169 PF03945 Endotoxin N48 264 PF01473 CW binding 1 346 365 PF01473 CW binding 1 388 406 PF01473CW binding 1 460 476 APG00407 Seq ID 170 PF05431 Toxin 10 208 401APG00408 Seq ID 171 PF03318 ETX MTX2 29 252 APG00409 Seq ID 172 PF01338Bac thur toxin 63 261 APG00412 Seq ID 173 PF05431 Toxin 10 197 390APG00413 Seq ID 174 PF00652 Ricin B lectin 36 162 PF05431 Toxin 10 172371 APG00415 Seq ID 175 PF05431 Toxin 10 154 347 APG00419 Seq ID 176PF05431 Toxin 10 203 396 APG00422 Seq ID 177 PF01338 Bac thur toxin 21244 APG00427 Seq ID 178 PF03318 ETX MTX2 83 314 APG00454 Seq ID 179PF05431 Toxin 10 235 431 APG00456 Seq ID 180 PF01338 Bac thur toxin 1200 APG00459 Seq ID 181 PF05431 Toxin 10 211 404 APG00461 Seq ID 182PF05431 Toxin 10 258 455 APG00462 Seq ID 183 PF01338 Bac thur toxin 7228 APG00467 Seq ID 184 PF03945 Endotoxin N 75 287 APG00499 Seq ID 185PF00652 Ricin B lectin 24 153 PF05431 Toxin 10 164 337 APG00500 Seq ID186 PF03945 Endotoxin N 57 301 APG00517 Seq ID 187 PF03318 ETX MTX2 72305 APG00521 Seq ID 188 PF05431 Toxin 10 194 387 APG00532 Seq ID 189PF14200 RicinB lectin 2 66 156 PF05431 Toxin 10 163 358 APG00550 Seq ID190 PF00652 Ricin B lectin 9 131 PF05431 Toxin 10 148 337 APG00559 SeqID 191 PF03318 ETX MTX2 6 224 APG00571 Seq ID 192 PF14200 RicinB lectin2 4 102 PF05431 Toxin 10 156 350 APG00590 Seq ID 193 PF03318 ETX MTX2 74294 APG00591 Seq ID 194 PF05431 Toxin 10 155 349 APG00592 Seq ID 195PF05431 Toxin 10 216 409 APG00596 Seq ID 196 PF00652 Ricin B lectin 37178 PF05431 Toxin 10 179 379 APG00600 Seq ID 197 PF05431 Toxin 10 207399 APG00603 Seq ID 198 PF03945 Endotoxin N 69 279 APG00619 Seq ID 199PF05431 Toxin 10 207 399 APG00635 Seq ID 201 PF03318 ETX MTX2 126 252APG00642 Seq ID 202 PF00652 Ricin B lectin 9 142 PF05431 Toxin 10 153347 APG00642 Seq ID 203 3′ Truncation PF00652 Ricin B lectin 9 142modified APG00647 Seq ID 204 PF01338 Bac thur toxin 50 275 APG00652 SeqID 205 PF05791 Bacillus HBL 40 232 APG00698 Seq ID 206 PF01338 Bac thurtoxin 52 280 APG00700 Seq ID 207 PF05431 Toxin 10 192 383 APG00723 SeqID 209 PF03945 Endotoxin N 171 379 APG00727 Seq ID 210 PF05431 Toxin 10187 379 APG00731 Seq ID 211 PF14200 RicinB lectin 2 47 150 PF05431 Toxin10 156 353 APG00732 Seq ID 212 PF00652 Ricin B lectin 24 153 PF05431Toxin 10 163 358 PF00388 PI-PLC-X 406 548 PF14200 RicinB lectin 2 837939 APG00741 Seq ID 213 PF00652 Ricin B lectin 39 160 PF05431 Toxin 10170 370 APG00748 Seq ID 214 PF14200 RicinB lectin 2 42 143 PF05431 Toxin10 149 346 APG00757 Seq ID 215 PF00652 Ricin B lectin 63 184 PF05431Toxin 10 194 394 APG00767 Seq ID 216 PF05431 Toxin 10 203 411 APG00768Seq ID 217 PF03318 ETX MTX2 99 335 APG00797 Seq ID 218 PF14200 RicinBlectin 2 43 147 PF05431 Toxin 10 153 347 APG00798 Seq ID 219 PF05431Toxin 10 216 408 APG00830 Seq ID 220 PF05791 Bacillus HBL 47 238APG00844 Seq ID 221 PF05431 Toxin 10 188 381 APG00851 Seq ID 222 PF03318ETX MTX2 82 313 APG00862 Seq ID 223 PF14200 RicinB lectin 2 2 57 PF05431Toxin 10 63 259 APG00898 Seq ID 224 PF03318 ETX MTX2 30 254 APG00907 SeqID 225 PF03318 ETX MTX2 28 266 APG00913 Seq ID 226 PF05431 Toxin 10 252449 APG00925 Seq ID 227 PF05431 Toxin 10 242 437 APG00074 Seq ID 228Alternate start PF12495 Vip3A N 12 187 modified APG00084 Seq ID 229Alternate start PF03945 Endotoxin N 66 290 modified PF00555 Endotoxin M295 519 PF03944 Endotoxin C 529 663 APG00108 Seq ID 230 Alternate startPF01338 Bac thur toxin 1 191 modified APG00113 Seq ID 231 Alternatestart PF03945 Endotoxin N 53 299 modified PF00030 Crystall 650 729PF00030 Crystall 730 811 PF14200 RicinB lectin 2 852 959 APG00113 Seq ID232 Alternate start PF03945 Endotoxin N 25 271 modified PF00030 Crystall622 701 PF00030 Crystall 702 783 PF14200 RicinB lectin 2 824 931APG00116 Seq ID 233 Alternate start PF03945 Endotoxin N 69 291 modifiedPF00555 Endotoxin M 296 499 PF03944 Endotoxin C 509 642 APG00116 Seq ID234 Alternate start PF03945 Endotoxin N 69 291 modified and 3′ PF00555Endotoxin M 296 499 Truncation PF03944 Endotoxin C 509 641 APG00117 SeqID 235 Alternate start PF01338 Bac thur toxin 378 572 modified APG00117Seq ID 236 Alternate start PF01338 Bac thur toxin 1 192 modifiedAPG00118 Seq ID 237 Alternate start PF05431 Toxin 10 195 392 modifiedAPG00121 Seq ID 238 Alternate start PF01338 Bac thur toxin 18 238modified APG00132 Seq ID 239 Signal peptide PF05431 Toxin 10 180 373modified removed APG00134 Seq ID 240 Alternate start PF01338 Bac thurtoxin 1 191 modified APG00137 Seq ID 241 Alternate start PF03318 ETXMTX2 14 249 modified APG00141 Seq ID 242 Signal peptide PF05431 Toxin 10149 349 modified removed APG00150 Seq ID 243 Alternate start PF12495Vip3A N 9 185 modified APG00153 Seq ID 244 Alternate start PF03945Endotoxin N 65 287 modified PF00555 Endotoxin M 292 495 PF03944Endotoxin C 505 634 APG00153 Seq ID 245 Alternate start PF03945Endotoxin N 65 287 modified and 3′ PF00555 Endotoxin M 292 495Truncation PF03944 Endotoxin C 505 633 APG00166 Seq ID 246 Alternatestart PF12495 Vip3A N 14 186 modified APG00168 Seq ID 247 Alternatestart PF01338 Bac thur toxin 22 244 modified APG00177 Seq ID 248Alternate start PF01338 Bac thur toxin 16 239 modified APG00186 Seq ID249 Alternate start PF01338 Bac thur toxin 378 572 modified APG00186 SeqID 250 Alternate start PF01338 Bac thur toxin 1 192 modified APG00194Seq ID 251 Alternate start PF01338 Bac thur toxin 10 206 modifiedAPG00196 Seq ID 252 Alternate start PF03945 Endotoxin N 55 299 modifiedPF00030 Crystall 650 729 PF00030 Crystall 730 811 PF14200 RicinB lectin2 852 959 APG00198 Seq ID 253 Alternate start PF01338 Bac thur toxin 1190 modified APG00200 Seq ID 254 Alternate start PF01338 Bac thur toxin282 482 modified APG00200 Seq ID 255 Alternate start PF01338 Bac thurtoxin 1 192 modified APG00203 Seq ID 256 Signal peptide PF05431 Toxin 10161 354 modified removed APG00209 Seq ID 257 Signal peptide PF05791Bacillus HBL 10 196 modified removed APG00210 Seq ID 258 Alternate startno PFAM modified domains APG00212 Seq ID 259 Alternate start PF05431Toxin 10 207 400 modified APG00212 Seq ID 260 Signal peptide PF05431Toxin 10 178 371 modified removed APG00213 Seq ID 261 Signal peptidePF05431 Toxin 10 161 358 modified removed APG00214 Seq ID 262 Alternatestart PF05791 Bacillus HBL 48 231 modified APG00219 Seq ID 263 Signalpeptide PF03945 Endotoxin N 78 299 modified removed APG00221 Seq ID 264Signal peptide PF05431 Toxin 10 165 358 modified removed APG00223 Seq ID265 Alternate start PF05431 Toxin 10 193 390 modified APG00223 Seq ID266 Signal peptide PF05431 Toxin 10 166 363 modified removed APG00227Seq ID 267 Alternate start PF03318 ETX MTX2 122 355 modified APG00227Seq ID 268 Signal peptide PF03318 ETX MTX2 93 326 modified removedAPG00229 Seq ID 269 Alternate start PF05431 Toxin 10 201 394 modifiedAPG00229 Seq ID 270 Signal peptide PF05431 Toxin 10 172 365 modifiedremoved APG00230 Seq ID 271 Alternate start PF00652 Ricin B lectin 35166 modified PF05431 Toxin 10 176 372 APG00231 Seq ID 272 Alternatestart PF14200 RicinB lectin 2 43 146 modified PF05431 Toxin 10 152 349APG00232 Seq ID 273 Signal peptide PF03945 Endotoxin N 72 272 modifiedremoved PF14200 RicinB lectin 2 522 624 APG00233 Seq ID 274 Signalpeptide PF03945 Endotoxin N 47 254 modified removed PF14200 RicinBlectin 2 366 475 APG00235 Seq ID 275 Alternate start PF03945 Endotoxin N102 320 modified PF14200 RicinB lectin 2 436 539 APG00237 Seq ID 276Signal peptide PF05431 Toxin 10 166 359 modified removed APG00239 Seq ID277 Signal peptide PF03945 Endotoxin N 114 337 modified removed PF01473CW binding 1 394 412 PF01473 CW binding 1 415 434 APG00241 Seq ID 278Alternate start PF05791 Bacillus HBL 34 215 modified APG00241 Seq ID 279Signal peptide PF05791 Bacillus HBL 9 190 modified removed APG00242 SeqID 280 Alternate start PF05431 Toxin 10 193 389 modified APG00243 Seq ID281 Signal peptide PF05431 Toxin 10 161 354 modified removed APG00248Seq ID 282 Alternate start PF05431 Toxin 10 191 383 modified APG00249Seq ID 283 Alternate start PF05431 Toxin 10 210 404 modified APG00249Seq ID 284 Signal peptide PF05431 Toxin 10 182 376 modified removedAPG00251 Seq ID 285 Signal peptide PF03945 Endotoxin N 45 251 modifiedremoved PF14200 RicinB lectin 2 363 472 APG00255 Seq ID 286 Signalpeptide PF03945 Endotoxin N 67 299 modified removed PF01473 CW binding 1346 364 PF14200 RicinB lectin 2 446 549 APG00261 Seq ID 287 Signalpeptide PF05431 Toxin 10 169 366 modified removed APG00262 Seq ID 288Alternate start PF05431 Toxin 10 235 429 modified APG00262 Seq ID 289Alternate start PF05431 Toxin 10 202 396 modified APG00264 Seq ID 290Alternate start PF05791 Bacillus HBL 37 217 modified APG00264 Seq ID 291Signal peptide PF05791 Bacillus HBL 11 191 modified removed APG00266 SeqID 292 Alternate start PF03945 Endotoxin N 126 319 modified PF01473 CWbinding 1 414 433 PF01473 CW binding 1 435 450 APG00267 Seq ID 293Alternate start PF03945 Endotoxin N 110 309 modified APG00267 Seq ID 294Signal peptide PF03945 Endotoxin N 72 269 modified removed APG00271 SeqID 295 Signal peptide PF05791 Bacillus HBL 1 178 modified removedAPG00273 Seq ID 296 Alternate start PF12495 Vip3A N 14 186 modifiedPF02018 CBM 4 9 543 656 APG00274 Seq ID 297 Alternate start PF05791Bacillus HBL 51 233 modified APG00274 Seq ID 298 Signal peptide PF05791Bacillus HBL 22 204 modified removed APG00275 Seq ID 299 Alternate startPF03945 Endotoxin N 5 129 modified APG00279 Seq ID 300 Alternate startPF05791 Bacillus HBL 65 181 modified APG00284 Seq ID 302 Alternate startPF14200 RicinB lectin 2 2 98 modified PF05431 Toxin 10 152 349 APG00285Seq ID 303 Signal peptide PF03945 Endotoxin N 70 300 modified removedPF01473 CW binding 1 369 383 PF01473 CW binding 1 418 432 PF01473 CWbinding 1 535 551 APG00286 Seq ID 304 Signal peptide PF05791 BacillusHBL 12 192 modified removed APG00287 Seq ID 305 Alternate start PF14200RicinB lectin 2 50 146 modified PF05431 Toxin 10 152 349 APG00288 Seq ID306 Signal peptide PF03945 Endotoxin N 77 280 modified removed PF01473CW binding 1 372 391 PF01473 CW binding 1 393 407 APG00290 Seq ID 307Alternate start PF01338 Bac thur toxin 18 244 modified APG00292 Seq ID309 Signal peptide PF03945 Endotoxin N 57 266 modified removed PF05588Botulinum 363 463 HA-17 APG00294 Seq ID 310 Signal peptide PF05791Bacillus HBL 17 200 modified removed APG00295 Seq ID 311 Alternate startPF05431 Toxin 10 218 414 modified APG00295 Seq ID 312 Signal peptidePF05431 Toxin 10 182 378 modified removed APG00297 Seq ID 313 Alternatestart PF05791 Bacillus HBL 36 216 modified APG00297 Seq ID 314 Signalpeptide PF05791 Bacillus HBL 10 190 modified removed APG00298 Seq ID 315Alternate start PF05791 Bacillus HBL 38 218 modified APG00298 Seq ID 316Signal peptide PF05791 Bacillus HBL 13 193 modified removed APG00301 SeqID 317 Signal peptide PF05431 Toxin 10 161 354 modified removed APG00302Seq ID 318 Alternate start PF03945 Endotoxin N 69 278 modified PF14200RicinB lectin 2 390 485 APG00302 Seq ID 319 Signal peptide PF03945Endotoxin N 61 270 modified removed PF14200 RicinB lectin 2 382 477APG00305 Seq ID 320 Signal peptide PF05791 Bacillus HBL 18 204 modifiedremoved APG00307 Seq ID 321 Alternate start PF05791 Bacillus HBL 32 216modified APG00307 Seq ID 322 Signal peptide PF05791 Bacillus HBL 8 188modified removed APG00308 Seq ID 323 Signal peptide PF05431 Toxin 10 174368 modified removed APG00314 Seq ID 324 Alternate start PF05431 Toxin10 154 348 modified APG00320 Seq ID 325 Alternate start PF05431 Toxin 10210 404 modified APG00340 Seq ID 326 Alternate start PF14200 RicinBlectin 2 42 143 modified PF05431 Toxin 10 149 347 APG00353 Seq ID 327Signal peptide PF05431 Toxin 10 161 354 modified removed APG00356 Seq ID328 Alternate start PF14200 RicinB lectin 2 50 146 modified PF05431Toxin 10 152 349 APG00368 Seq ID 329 Alternate start PF14200 RicinBlectin 2 2 98 modified PF05431 Toxin 10 152 350 APG00369 Seq ID 330Signal peptide PF05431 Toxin 10 182 376 modified removed APG00372 Seq ID331 Signal peptide PF03945 Endotoxin N 80 265 modified removed PF01473CW binding 1 381 400 PF01473 CW binding 1 402 416 APG00373 Seq ID 332Alternate start PF00652 Ricin B lectin 37 164 modified PF05431 Toxin 10175 371 APG00375 Seq ID 333 Signal peptide PF03945 Endotoxin N 79 282modified removed PF01473 CW binding 1 420 437 APG00376 Seq ID 334 Signalpeptide PF05431 Toxin 10 165 357 modified removed APG00377 Seq ID 335Alternate start PF14200 RicinB lectin 2 43 146 modified PF05431 Toxin 10152 349 APG00379 Seq ID 336 Alternate start PF14200 RicinB lectin 2 47148 modified PF05431 Toxin 10 156 353 APG00379 Seq ID 337 Alternatestart PF14200 RicinB lectin 2 43 144 modified PF05431 Toxin 10 152 349APG00385 Seq ID 338 Signal peptide PF05431 Toxin 10 176 369 modifiedremoved APG00391 Seq ID 339 Signal peptide PF03945 Endotoxin N 72 269modified removed APG00392 Seq ID 340 Signal peptide PF05431 Toxin 10 176369 modified removed APG00395 Seq ID 341 Signal peptide PF03945Endotoxin N 70 294 modified removed PF01473 CW binding 1 395 411 PF01473CW binding 1 423 439 APG00396 Seq ID 342 Signal peptide PF03945Endotoxin N 76 283 modified removed PF01473 CW binding 1 363 380 PF01473CW binding 1 382 401 PF01473 CW binding 1 485 500 APG00398 Seq ID 343Alternate start PF05431 Toxin 10 210 407 modified APG00398 Seq ID 344Signal peptide PF05431 Toxin 10 182 379 modified removed APG00401 Seq ID345 Signal peptide PF03945 Endotoxin N 66 298 modified removed PF01473CW binding 1 345 363 PF14200 RicinB lectin 2 445 548 APG00407 Seq ID 346Signal peptide PF05431 Toxin 10 181 374 modified removed APG00409 Seq ID347 Alternate start PF01338 Bac thur toxin 2 198 modified APG00412 SeqID 348 Alternate start PF05431 Toxin 10 188 381 modified APG00412 Seq ID349 Signal peptide PF05431 Toxin 10 161 354 modified removed APG00413Seq ID 350 Alternate start PF00652 Ricin B lectin 35 161 modifiedPF05431 Toxin 10 171 370 APG00415 Seq ID 351 Alternate start PF05431Toxin 10 153 346 modified APG00419 Seq ID 352 Signal peptide PF05431Toxin 10 174 367 modified removed APG00427 Seq ID 353 Signal peptidePF03318 ETX MTX2 52 283 modified removed PF03318 ETX MTX2 52 283APG00454 Seq ID 354 Alternate start PF05431 Toxin 10 193 389 modifiedAPG00459 Seq ID 355 Signal peptide PF05431 Toxin 10 182 375 modifiedremoved APG00499 Seq ID 356 Alternate start PF00652 Ricin B lectin 24153 modified PF05431 Toxin 10 164 337 APG00500 Seq ID 357 Alternatestart PF03945 Endotoxin N 54 298 modified APG00517 Seq ID 358 Alternatestart PF03318 ETX MTX2 51 284 modified APG00521 Seq ID 359 Signalpeptide PF05431 Toxin 10 167 360 modified removed APG00559 Seq ID 360Alternate start PF03318 ETX MTX2 6 224 modified APG00559 Seq ID 361Alternate start PF03318 ETX MTX2 16 250 modified APG00571 Seq ID 362Alternate start PF14200 RicinB lectin 2 3 98 modified PF05431 Toxin 10152 346 APG00590 Seq ID 363 Signal peptide PF03318 ETX MTX2 43 264modified removed APG00592 Seq ID 364 Alternate start PF05431 Toxin 10207 400 modified APG00592 Seq ID 365 Signal peptide PF05431 Toxin 10 178371 modified removed APG00596 Seq ID 366 Alternate start PF00652 Ricin Blectin 37 178 modified PF05431 Toxin 10 179 379 APG00600 Seq ID 367Signal peptide PF05431 Toxin 10 178 370 modified removed APG00619 Seq ID368 Signal peptide PF05431 Toxin 10 178 370 modified removed APG00635Seq ID 369 Alternate start PF03318 ETX MTX2 108 233 modified APG00635Seq ID 370 Alternate start PF03318 ETX MTX2 85 222 modified APG00635 SeqID 371 Signal peptide PF03318 ETX MTX2 77 214 modified removed APG00647Seq ID 372 Alternate start PF01338 Bac thur toxin 17 243 modifiedAPG00652 Seq ID 373 Signal peptide PF05791 Bacillus HBL 10 202 modifiedremoved APG00698 Seq ID 374 Alternate start PF01338 Bac thur toxin 19248 modified APG00723 Seq ID 375 Alternate start PF03945 Endotoxin N 134342 modified APG00731 Seq ID 376 Alternate start PF14200 RicinB lectin 243 146 modified PF05431 Toxin 10 152 349 APG00748 Seq ID 377 Alternatestart PF14200 RicinB lectin 2 42 143 modified PF05431 Toxin 10 149 346APG00757 Seq ID 378 Alternate start PF00652 Ricin B lectin 39 160modified PF05431 Toxin 10 170 370 APG00767 Seq ID 379 Signal peptidePF05431 Toxin 10 174 382 modified removed APG00768 Seq ID 380 Signalpeptide PF03318 ETX MTX2 70 306 modified removed APG00798 Seq ID 381Alternate start PF05431 Toxin 10 207 399 modified APG00798 Seq ID 382Signal peptide PF05431 Toxin 10 178 370 modified removed APG00830 Seq ID383 Alternate start PF05791 Bacillus HBL 42 233 modified APG00830 Seq ID384 Signal peptide PF05791 Bacillus HBL 12 203 modified removed APG00844Seq ID 385 Signal peptide PF05431 Toxin 10 161 354 modified removedAPG00851 Seq ID 386 Signal peptide PF03318 ETX MTX2 30 283 modifiedremoved APG00898 Seq ID 387 Alternate start PF03318 ETX MTX2 23 247modified APG00907 Seq ID 388 Alternate start PF03318 ETX MTX2 26 264modified APG00913 Seq ID 389 Alternate start PF05431 Toxin 10 210 407modified APG00925 Seq ID 390 Alternate start PF05431 Toxin 10 209 404modified APG00568 Seq ID 391 PF14200 RicinB lectin 2 49 150 PF05431Toxin 10 156 353 APG00568 Seq ID 392 Alternate start PF14200 RicinBlectin 2 45 146 modified PF05431 Toxin 10 152 349 APG00716 Seq ID 393PF05431 Toxin 10 192 385 APG00716 Seq ID 394 Signal peptide PF05431Toxin 10 165 358 modified removed APG00736 Seq ID 395 PF01338 Bac thurtoxin 100 326 APG00736 Seq ID 396 Alternate start PF01338 Bac thur toxin19 245 modified APG00930 Seq ID 397 PF03318 ETX MTX2 29 250 APG01245 SeqID 398 PF03318 ETX MTX2 30 250

Recombinant or synthetic nucleic acids encoding the pesticidalpolypeptides disclosed herein are also provided. Of particular interestare nucleic acid sequences that have been designed for expression in aplant of interest. That is, the nucleic acid sequence can be optimizedfor increased expression in a host plant. A pesticidal protein of theinvention can be back-translated to produce a nucleic acid comprisingcodons optimized for expression in a particular host, for example, acrop plant. In another embodiment, the polynucleotides encoding thepolypeptides provided herein may be optimized for increased expressionin the transformed plant. That is, the polynucleotides can besynthesized using plant-preferred codons for improved expression. See,for example, Campbell and Gowri (1990) Plant Physiol. 92:1-11 for adiscussion of host-preferred codon usage. Methods are available in theart for synthesizing plant-preferred genes. See, for example, U.S. Pat.Nos. 5,380,831, and 5,436,391, and Murray et al. (1989) Nucleic AcidsRes. 17:477-498, herein incorporated by reference. Expression of such acoding sequence by the transformed plant (e.g., dicot or monocot) willresult in the production of a pesticidal polypeptide and conferincreased resistance in the plant to a pest. Recombinant and syntheticnucleic acid molecules encoding the pesticidal proteins of the inventiondo not include the naturally occurring bacterial sequence encoding theprotein.

A “recombinant polynucleotide” or “recombinant nucleic acid” comprises acombination of two or more chemically linked nucleic acid segments whichare not found directly joined in nature. By “directly joined” isintended the two nucleic acid segments are immediately adjacent andjoined to one another by a chemical linkage. In specific embodiments,the recombinant polynucleotide comprises a polynucleotide of interest ora variant or fragment thereof such that an additional chemically linkednucleic acid segment is located either 5′, 3′ or internal to thepolynucleotide of interest. Alternatively, the chemically-linked nucleicacid segment of the recombinant polynucleotide can be formed by deletionof a sequence. The additional chemically linked nucleic acid segment orthe sequence deleted to join the linked nucleic acid segments can be ofany length, including for example, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20or greater nucleotides.

Various methods for making such recombinant polynucleotides includechemical synthesis or by the manipulation of isolated segments ofpolynucleotides by genetic engineering techniques. In specificembodiments, the recombinant polynucleotide can comprise a recombinantDNA sequence or a recombinant RNA sequence. A “fragment of a recombinantpolynucleotide or nucleic acid” comprises at least one of a combinationof two or more chemically linked amino acid segments which are not founddirectly joined in nature.

Fragments of a polynucleotide (RNA or DNA) may encode protein fragmentsthat retain activity. In specific embodiments, a fragment of arecombinant polynucleotide or a recombinant polynucleotide constructcomprises at least one junction of the two or more chemically linked oroperably linked nucleic acid segments which are not found directlyjoined in nature. A fragment of a polynucleotide that encodes abiologically active portion of a polypeptide that retains pesticidalactivity will encode at least 25, 30, 40, 50, 60, 70, 75, 80, 90, 100,110, 120, 125, 130, 140, 150, 160, 170, 175, 180, contiguous aminoacids, or up to the total number of amino acids present in a full-lengthpolypeptide as set forth in SEQ ID NO:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29,30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47,48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83,84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100,101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128,129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142,143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170,171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184,185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198,199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212,213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226,227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254,255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268,269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282,283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352,353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366,367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380,381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394,395, 396, 397, or 398. In specific embodiments, such polypeptidefragments are active fragments, and in still other embodiments, thepolypeptide fragment comprises a recombinant polypeptide fragment. Asused herein, a fragment of a recombinant polypeptide comprises at leastone of a combination of two or more chemically linked amino acidsegments which are not found directly joined in nature.

By “variants” is intended to mean substantially similar sequences. Forpolynucleotides, a variant comprises a deletion and/or addition of oneor more nucleotides at one or more internal sites within the nativepolynucleotide and/or a substitution of one or more nucleotides at oneor more sites in the native polynucleotide. As used herein, a “native”polynucleotide or polypeptide comprises a naturally occurring nucleotidesequence or amino acid sequence, respectively.

Variants of a particular polynucleotide of the invention (i.e., thereference polynucleotide) can also be evaluated by comparison of thepercent sequence identity between the polypeptide encoded by a variantpolynucleotide and the polypeptide encoded by the referencepolynucleotide. Thus, for example, an isolated polynucleotide thatencodes a polypeptide with a given percent sequence identity to thepolypeptide of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68,69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103,104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117,118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131,132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143, 144, 145,146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159,160, 161, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173,174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187,188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201,202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215,216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229,230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243,244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271,272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285,286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299,300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312, 313,314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325, 326, 327,328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339, 340, 341,342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355,356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369,370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383,384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, or398 are disclosed. Percent sequence identity between any twopolypeptides can be calculated using sequence alignment programs andparameters described elsewhere herein. Where any given pair ofpolynucleotides of the invention is evaluated by comparison of thepercent sequence identity shared by the two polypeptides they encode,the percent sequence identity between the two encoded polypeptides is atleast about 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 81%, 82%, 83%,84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%,98%, 99% or more sequence identity to SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, or 398. In other embodiments, the variant ofthe polynucleotide provided herein differs from the native sequence byat least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more nucleotides.

Variant polynucleotide and proteins also encompass sequences andproteins derived from a mutagenic and recombinogenic procedure such asDNA shuffling. With such a procedure, one or more different pesticidalprotein disclosed herein (SEQ ID NO: 1-209) is manipulated to create anew pesticidal protein possessing the desired properties. In thismanner, libraries of recombinant polynucleotides are generated from apopulation of related sequence polynucleotides comprising sequenceregions that have substantial sequence identity and can be homologouslyrecombined in vitro or in vivo. For example, using this approach,sequence motifs encoding a domain of interest may be shuffled betweenthe pesticidal sequences provided herein and other known pesticidalgenes to obtain a new gene coding for a protein with an improvedproperty of interest, such as an increased K_(m) in the case of anenzyme. Strategies for such DNA shuffling are known in the art. See, forexample, Stemmer (1994) Proc. Natl. Acad. Sci. USA 91:10747-10751;Stemmer (1994) Nature 370:389-391; Crameri et al. (1997) Nature Biotech.15:436-438; Moore et al. (1997) J. Mol. Biol. 272:336-347; Zhang et al.(1997) Proc. Natl. Acad. Sci. USA 94:4504-4509; Crameri et al. (1998)Nature 391:288-291; and U.S. Pat. Nos. 5,605,793 and 5,837,458. A“shuffled” nucleic acid is a nucleic acid produced by a shufflingprocedure such as any shuffling procedure set forth herein. Shufflednucleic acids are produced by recombining (physically or virtually) twoor more nucleic acids (or character strings), for example in anartificial, and optionally recursive, fashion. Generally, one or morescreening steps are used in shuffling processes to identify nucleicacids of interest; this screening step can be performed before or afterany recombination step. In some (but not all) shuffling embodiments, itis desirable to perform multiple rounds of recombination prior toselection to increase the diversity of the pool to be screened. Theoverall process of recombination and selection are optionally repeatedrecursively. Depending on context, shuffling can refer to an overallprocess of recombination and selection, or, alternately, can simplyrefer to the recombinational portions of the overall process.

In one embodiment, a method of obtaining a polynucleotide that encodesan improved polypeptide comprising pesticidal activity is provided,wherein the improved polypeptide has at least one improved property overany one of SEQ ID NOS: 1-229. Such methods can comprise (a) recombininga plurality of parental polynucleotides to produce a library ofrecombinant polynucleotides encoding recombinant pesticidalpolypeptides; (b) screening the library to identify a recombinantpolynucleotide that encodes an improved recombinant pesticidalpolypeptide that has an enhanced property improved over the parentalpolynucleotide; (c) recovering the recombinant polynucleotide thatencodes the improved recombinant pesticidal polypeptide identified in(b); and, (d) repeating steps (a), (b) and (c) using the recombinantpolynucleotide recovered in step (c) as one of the plurality of parentalpolynucleotides in repeated step (a).

iii. Sequence Comparisons

As used herein, the term “identity” or “percent identity” when used withrespect to a particular pair of aligned amino acid sequences, refers tothe percent amino acid sequence identity that is obtained by countingthe number of identical matches in the alignment and dividing suchnumber of identical matches by the length of the aligned sequences. Asused herein, the term “similarity” or “percent similarity” when usedwith respect to a particular pair of aligned amino acid sequences,refers to the sum of the scores that are obtained from a scoring matrixfor each amino acid pair in the alignment divided by the length of thealigned sequences.

Unless otherwise stated, identity and similarity will be calculated bythe Needleman-Wunsch global alignment and scoring algorithms (Needlemanand Wunsch (1970) J. Mol. Biol. 48(3):443-453) as implemented by the“needle” program, distributed as part of the EMBOSS software package(Rice, P. Longden, I. and Bleasby, A., EMBOSS: The European MolecularBiology Open Software Suite, 2000, Trends in Genetics 16, (6) pp276-277, versions 6.3.1 available from EMBnet atembnet.org/resource/emboss and emboss.sourceforge.net, among othersources) using default gap penalties and scoring matrices (EBLOSUM62 forprotein and EDNAFULL for DNA). Equivalent programs may also be used. By“equivalent program” is intended any sequence comparison program that,for any two sequences in question, generates an alignment havingidentical nucleotide residue matches and an identical percent sequenceidentity when compared to the corresponding alignment generated byneedle from EMBOSS version 6.3.1.

Additional mathematical algorithms are known in the art and can beutilized for the comparison of two sequences. See, for example, thealgorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA87:2264, modified as in Karlin and Altschul (1993) Proc. Natl. Acad.Sci. USA 90:5873-5877. Such an algorithm is incorporated into the BLASTprograms of Altschul et al. (1990) J. Mol. Biol. 215:403. BLASTnucleotide searches can be performed with the BLASTN program (nucleotidequery searched against nucleotide sequences) to obtain nucleotidesequences homologous to pesticidal-like nucleic acid molecules of theinvention, or with the BLASTX program (translated nucleotide querysearched against protein sequences) to obtain protein sequenceshomologous to pesticidal nucleic acid molecules of the invention. BLASTprotein searches can be performed with the BLASTP program (protein querysearched against protein sequences) to obtain amino acid sequenceshomologous to pesticidal protein molecules of the invention, or with theTBLASTN program (protein query searched against translated nucleotidesequences) to obtain nucleotide sequences homologous to pesticidalprotein molecules of the invention. To obtain gapped alignments forcomparison purposes, Gapped BLAST (in BLAST 2.0) can be utilized asdescribed in Altschul et al. (1997) Nucleic Acids Res. 25:3389.Alternatively, PSI-Blast can be used to perform an iterated search thatdetects distant relationships between molecules. See Altschul et al.(1997) supra. When utilizing BLAST, Gapped BLAST, and PSI-Blast isprograms, the default parameters of the respective programs (e.g.,BLASTX and BLASTN) can be used. Alignment may also be performed manuallyby inspection.

Two sequences are “optimally aligned” when they are aligned forsimilarity scoring using a defined amino acid substitution matrix (e.g.,BLOSUM62), gap existence penalty and gap extension penalty so as toarrive at the highest score possible for that pair of sequences. Aminoacid substitution matrices and their use in quantifying the similaritybetween two sequences are well-known in the art and described, e.g., inDayhoff et al. (1978) “A model of evolutionary change in proteins.” In“Atlas of Protein Sequence and Structure,” Vol. 5, Suppl. 3 (ed. M. O.Dayhoff), pp. 345-352. Natl. Biomed. Res. Found., Washington, D.C. andHenikoff et al. (1992) Proc. Natl. Acad. Sci. USA 89:10915-10919. TheBLOSUM62 matrix is often used as a default scoring substitution matrixin sequence alignment protocols. The gap existence penalty is imposedfor the introduction of a single amino acid gap in one of the alignedsequences, and the gap extension penalty is imposed for each additionalempty amino acid position inserted into an already opened gap. Thealignment is defined by the amino acids positions of each sequence atwhich the alignment begins and ends, and optionally by the insertion ofa gap or multiple gaps in one or both sequences, so as to arrive at thehighest possible score.

While optimal alignment and scoring can be accomplished manually, theprocess is facilitated by the use of a computer-implemented alignmentalgorithm, e.g., gapped BLAST 2.0, described in Altschul et al. (1997)Nucleic Acids Res. 25:3389-3402, and made available to the public at theNational Center for Biotechnology Information Website(www.ncbi.nlm.nih.gov). Optimal alignments, including multiplealignments, can be prepared using, e.g., PSI-BLAST, available throughwww.ncbi.nlm.nih.gov and described by Altschul et al. (1997) NucleicAcids Res. 25:3389-3402.

With respect to an amino acid sequence that is optimally aligned with areference sequence, an amino acid residue “corresponds to” the positionin the reference sequence with which the residue is paired in thealignment. The “position” is denoted by a number that sequentiallyidentifies each amino acid in the reference sequence based on itsposition relative to the N-terminus. For example, in SEQ ID NO: 177position 1 is M, position 2 is N, position 3 is E, etc. When a testsequence is optimally aligned with SEQ ID NO: 177, a residue in the testsequence that aligns with the E at position 3 is said to “correspond toposition 3” of SEQ ID NO: 177. Owing to deletions, insertion,truncations, fusions, etc., that must be taken into account whendetermining an optimal alignment, in general the amino acid residuenumber in a test sequence as determined by simply counting from theN-terminal will not necessarily be the same as the number of itscorresponding position in the reference sequence. For example, in a casewhere there is a deletion in an aligned test sequence, there will be noamino acid that corresponds to a position in the reference sequence atthe site of deletion. Where there is an insertion in an alignedreference sequence, that insertion will not correspond to any amino acidposition in the reference sequence. In the case of truncations orfusions there can be stretches of amino acids in either the reference oraligned sequence that do not correspond to any amino acid in thecorresponding sequence.

iv. Antibodies

Antibodies to the polypeptides of the present invention, or to variantsor fragments thereof, are also encompassed. Methods for producingantibodies are well known in the art (see, for example, Harlow and Lane(1988) Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory,Cold Spring Harbor, N.Y.; and U.S. Pat. No. 4,196,265). These antibodiescan be used in kits for the detection and isolation of toxinpolypeptides. Thus, this disclosure provides kits comprising antibodiesthat specifically bind to the polypeptides described herein, including,for example, polypeptides having the sequence of SEQ ID NOs: 1, 2, 3, 4,5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23,24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41,42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59,60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77,78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95,96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124,125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138,139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152,153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180,181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194,195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208,209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222,223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236,237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250,251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264,265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278,279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306,307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320,321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334,335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362,363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376,377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390,391, 392, 393, 394, 395, 396, 397, or 398.

II. Pests

The compositions and methods provided herein are useful against avariety of pests. “Pests” includes but is not limited to, insects,fungi, bacteria, nematodes, acarids, protozoan pathogens,animal-parasitic liver flukes, and the like. Pests of particularinterest are insect pests, particularly insect pests that causesignificant damage to agricultural plants. Insect pests include insectsselected from the orders Coleoptera, Diptera, Hymenoptera, Lepidoptera,Mallophaga, Homoptera, Hemiptera, Orthroptera, Thysanoptera, Dermaptera,Isoptera, Anoplura, Siphonaptera, Trichoptera, or nematodes. Innon-limiting embodiments, the insect pest comprises Western cornrootworm, Diabrotica virgifera virgifera; Fall armyworm, Spodopterafrupperda; Colorado potato beetle, Leptinotarsa decemlineata; Cornearworm, Helicoverpa zea (in North America same species attacks cottonand called cotton bollworm); European corn borer, Ostrinia nubilalis;Black cutworm, Agrotis Ipsilon; Diamondback moth, Plutella xylostella;Velvetbean caterpillar, Anticarsia gemmatalis; Southwestern corn borer,Diatraea grandiosealla; Cotton bollworm, Helicoverpa armigera (foundother than USA in rest of the world); Southern green stinkbug, Nezaraviridula; Green stinkbug, Chinavia halaris; Brown marmorated stinkbug,Halyomorpha halys; and Brown stinbug, Euschistus servus Euschistus heros(Neotropical brown stink bug OR soy stink bug); Piezodorus guildinii(red-banded stink bug); Dichelops melacanthus (no common name) and/orDichelops furcatus (no common name); an aphid, such as a soybean aphid.In other embodiments, the pest comprises a nematode including, but notlimited to, Meloidogyne hapla (Northern root-knot nematode); Meloidogyneenterolobii, Meloidogyne arenaria (peanut root-knot nematode); andMeloidogyne javanica.

The term “insect pests” as used herein refers to insects and othersimilar pests such as, for example, those of the order Acari including,but not limited to, mites and ticks. Insect pests of the presentinvention include, but are not limited to, insects of the orderLepidoptera, e.g. Achoroia grisella, Acleris gloverana, Acleris variana,Adoxophyes orana, Agrotis ipsilon, Alabama argillacea, Alsophilapometaria, Amyelois transitella, Anagasta kuehniella, Anarsialineatella, Anisota senatoria, Antheraea pernyi, Anticarsia gemmatalis,Archips sp., Argyrotaenia sp., Athetis mindara, Bombyx mori, Bucculatrixthurberiella, Cadra cautella, Choristoneura sp., Cochylls hospes, Coliaseurytheme, Corcyra cephalonica, Cydia latiferreanus, Cydia pomonella,Datana integerrima, Dendrolimus sibericus, Desmiafeneralis, Diaphaniahyalinata, Diaphania nitidalis, Diatraea grandiosella, Diatraeasaccharalis, Ennomos subsignaria, Eoreuma loftini, Esphestia elutella,Erannis tilaria, Estigmene acrea, Eulia salubricola, Eupocoelliaambiguella, Eupoecilia ambiguella, Euproctis chrysorrhoea, Euxoamessoria, Galleria mellonella, Grapholita molesta, Harrisina americana,Helicoverpa subflexa, Helicoverpa zea, Heliothis virescens, Hemileucaoliviae, Homoeosoma electellum, Hyphantia cunea, Keiferialycopersicella, Lambdina fiscellaria fiscellaria, Lambdina fiscellarialugubrosa, Leucoma salicis, Lobesia botrana, Loxostege sticticalis,Lymantria dispar, Macalla thyrisalis, Malacosoma sp., Mamestrabrassicae, Mamestra configurata, Manduca quinquemaculata, Manduca sexta,Maruca testulalis, Melanchra picta, Operophtera brumata, Orgyia sp.,Ostrinia nubilalis, Paleacrita vernata, Papilio cresphontes,Pectinophora gossypiella, Phryganidia californica, Phyllonorycterblancardella, Pieris napi, Pieris rapae, Plathypena scabra, Platynotaflouendana, Platynota stultana, Platyptilia carduidactyla, Plodiainterpunctella, Plutella xylostella, Pontia protodice, Pseudaletiaunipuncta, Pseudoplasia includens, Sabulodes aegrotata, Schizuraconcinna, Sitotroga cerealella, Spilonta ocellana, Spodoptera sp.,Thaurnstopoea pityocampa, Tinsola bisselliella, Trichoplusia hi, Udearubigalis, Xylomyges curiails, and Yponomeuta padella.

Insect pests also include insects selected from the orders Diptera,Hymenoptera, Lepidoptera, Mallophaga, Homoptera, Hemiptera, Orthroptera,Thysanoptera, Dermaptera, Isoptera, Anoplura, Siphonaptera, Trichoptera,Coleoptera.

Insect pests of the invention for the major crops include, but are notlimited to: Maize: Ostrinia nubilalis, European corn borer; Agrotisipsilon, black cutworm; Helicoverpa zeae, corn earworm; Spodopterafrugiperda, fall armyworm; Diatraea grandiosella, southwestern cornborer; Elasmopalpus lignosellus, lesser cornstalk borer; Diatraeasaccharalis, surgarcane borer; western corn rootworm, e.g., Diabroticavirgifera virgifera; northern corn rootworm, e.g., Diabroticalongicornis barberi; southern corn rootworm, e.g., Diabroticaundecimpunctata howardi; Melanotus spp., wireworms; Cyclocephalaborealis, northern masked chafer (white grub); Cyclocephala immaculata,southern masked chafer (white grub); Popillia japonica, Japanese beetle;Chaetocnema pulicaria, corn flea beetle; Sphenophorus maidis, maizebillbug; Rhopalosiphum maidis, corn leaf aphid; Anuraphis maidiradicis,corn root aphid; Blissus leucopterus leucopterus, chinch bug; Melanoplusfemurrubrum, redlegged grasshopper; Melanoplus sanguinipes, migratorygrasshopper; Hylemya platura, seedcorn maggot; Agromyza parvicornis,corn blotch leafminer; Anaphothrips obscrurus, grass thrips; Solenopsismilesta, thief ant; Tetranychus urticae, two spotted spider mite;Sorghum: Chilo partellus, sorghum borer; Spodoptera frugiperda, fallarmyworm; Helicoverpa zea, corn earworm; Elasmopalpus lignosellus,lesser cornstalk borer; Feltia subterranea, granulate cutworm;Phyllophaga crinita, white grub; Eleodes, Conoderus, and Aeolus spp.,wireworms; Oulema melanopus, cereal leaf beetle; Chaetocnema pulicaria,corn flea beetle; Sphenophorus maidis, maize billbug; Rhopalosiphummaidis; corn leaf aphid; Sipha flava, yellow sugarcane aphid; chinchbug, e.g., Blissus leucopterus leucopterus; Contarinia sorghicola,sorghum midge; Tetranychus cinnabarinus, carmine spider mite;Tetranychus urticae, two-spotted spider mite; Wheat: Pseudaletiaunipunctata, army worm; Spodoptera frugiperda, fall armyworm;Elasmopalpus lignosellus, lesser cornstalk borer; Agrotis orthogonia,pale western cutworm; Elasmopalpus lignosellus, lesser cornstalk borer;Oulema melanopus, cereal leaf beetle; Hypera punctata, clover leafweevil; southern corn rootworm, e.g., Diabrotica undecimpunctatahowardi; Russian wheat aphid; Schizaphis graminum, greenbug; Macrosiphumavenae, English grain aphid; Melanoplus femurrubrum, redleggedgrasshopper; Melanoplus differentialis, differential grasshopper;Melanoplus sanguinipes, migratory grasshopper; Mayetiola destructor,Hessian fly; Sitodiplosis mosellana, wheat midge; Meromyza americana,wheat stem maggot; Hylemya coarctata, wheat bulb fly; Frankliniellafusca, tobacco thrips; Cephus cinctus, wheat stem sawfly; Aceriatulipae, wheat curl mite; Sunflower: Cylindrocupturus adspersus,sunflower stem weevil; Smicronyx fulus, red sunflower seed weevil;Smicronyx sordidus, gray sunflower seed weevil; Suleima helianthana,sunflower bud moth; Homoeosoma electellum, sunflower moth; Zygogrammaexclamationis, sunflower beetle; Bothyrus gibbosus, carrot beetle;Neolasioptera murtfeldtiana, sunflower seed midge; Cotton: Heliothisvirescens, tobacco budworm; Helicoverpa zea, cotton bollworm; Spodopteraexigua, beet armyworm; Pectinophora gossypiella, pink bollworm; bollweevil, e.g., Anthonomus grandis; Aphis gossypii, cotton aphid;Pseudatomoscelis seriatus, cotton fleahopper; Trialeurodes abutilonea,bandedwinged whitefly; Lygus lineolaris, tarnished plant bug; Melanoplusfemurrubrum, redlegged grasshopper; Melanoplus differentialis,differential grasshopper; Thrips tabaci, onion thrips; Franklinkiellafusca, tobacco thrips; Tetranychus cinnabarinus, carmine spider mite;Tetranychus urticae, two-spotted spider mite; Rice: Diatraeasaccharalis, sugarcane borer; Spodoptera frupperda, fall armyworm;Helicoverpa zea, corn earworm; Colaspis brunnea, grape colaspis;Lissorhoptrus oryzophilus, rice water weevil; Sitophilus oryzae, riceweevil; Nephotettix nigropictus, rice leafhoper; chinch bug, e.g.,Blissus leucopterus leucopterus; Acrosternum hilare, green stink bug;Soybean: Pseudoplusia includens, soybean looper; Anticarsia gemmatalis,velvetbean caterpillar; Plathypena scabra, green cloverworm; Ostrinianubilalis, European corn borer; Agrotis ipsilon, black cutworm;Spodoptera exigua, beet armyworm; Heliothis virescens, tobacco budworm;Helicoverpa zea, cotton bollworm; Epilachna varivestis, Mexican beanbeetle; Myzus persicae, green peach aphid; Empoasca fabae, potatoleafhopper; Acrosternum hilare, green stink bug; Melanoplus femurrubrum,redlegged grasshopper; Melanoplus differentialis, differentialgrasshopper; Hylemya platura, seedcorn maggot; Sericothrips variabilis,soybean thrips; Thrips tabaci, onion thrips; Tetranychus turkestani,strawberry spider mite; Tetranychus urticae, two-spotted spider mite;Barley: Ostrinia nubilalis, European corn borer; Agrotis ipsilon, blackcutworm; Schizaphis graminum, greenbug; chinch bug, e.g., Blissusleucopterus leucopterus; Acrosternum hilare, green stink bug; Euschistusservus, brown stink bug; Jylemya platura, seedcorn maggot; Mayetioladestructor, Hessian fly; Petrobia latens, brown wheat mite; Oil SeedRape: Vrevicoryne brassicae, cabbage aphid; Phyllotreta cruciferae,crucifer flea beetle; Phyllotreta striolata, striped flea beetle;Phyllotreta nemorum, striped turnip flea beetle; Meligethes aeneus,rapeseed beetle; and the pollen beetles Meligethes rufimanus, Meligethesnigrescens, Meligethes canadianus, and Meligethes viridescens; Potato:Leptinotarsa decemlineata, Colorado potato beetle.

The methods and compositions provided herein may be effective againstHemiptera such as Lygus hesperus, Lygus lineolaris, Lygus pratensis,Lygus ruguhpennis Popp, Lygus pabulinus, Calocoris norvegicus, Orthopscompestris, Plesiocoris rugicollis, Cyrtopeltis modestus, Cyrtopeltisnotatus, Spanagonicus albofasciatus, Diaphnocoris chlorinonis,Labopidicola allii, Pseudatomoscelis seriatus, Adelphocoris rapidus,Poecilocapsus lineatus, Blissus leucopterus, Nysius ericae, Nysiusraphanus, Euschistus servus, Nezara viridula, Eurygaster, Coreidae,Pyrrhocoridae, Tinidae, Blostomatidae, Reduviidae, and Cimicidae. Pestsof interest also include Araecerus fasciculatus, coffee bean weevil;Acanthoscelides obtectus, bean weevil; Bruchus rufmanus, broadbeanweevil; Bruchus pisorum, pea weevil; Zabrotes subfasciatus, Mexican beanweevil; Diabrotica balteata, banded cucumber beetle; Cerotomatrifurcata, bean leaf beetle; Diabrotica virgifera, Mexican cornrootworm; Epitrix cucumeris, potato flea beetle; Chaetocnema confinis,sweet potato flea beetle; Hypera postica, alfalfa weevil; Anthonomusquadrigibbus, apple curculio; Sternechus paludatus, bean stalk weevil;Hypera brunnipennis, Egyptian alfalfa weevil; Sitophilus granaries,granary weevil; Craponius inaequalis, grape curculio; Sitophiluszeamais, maize weevil; Conotrachelus nenuphar, plum curculio; Euscepespostfaciatus, West Indian sweet potato weevil; Maladera castanea,Asiatic garden beetle; Rhizotrogus majalis, European chafer;Macrodactylus subspinosus, rose chafer; Tribolium confusum, confusedflour beetle; Tenebrio obscurus, dark mealworm; Tribolium castaneum, redflour beetle; Tenebrio molitor, yellow mealworm.

Nematodes include parasitic nematodes such as root-knot, cyst, andlesion nematodes, including Heterodera spp., Meloidogyne spp., andGlobodera spp.; particularly members of the cyst nematodes, including,but not limited to, Heterodera glycines (soybean cyst nematode);Heterodera schachtii (beet cyst nematode); Heterodera avenae (cerealcyst nematode); and Globodera rostochiensis and Globodera pailida(potato cyst nematodes). Lesion nematodes include Pratylenchus spp.

Insect pests may be tested for pesticidal activity of compositions ofthe invention in early developmental stages, e.g., as larvae or otherimmature forms. The insects may be reared in total darkness at fromabout 20° C. to about 30° C. and from about 30% to about 70% relativehumidity. Bioassays may be performed as described in Czapla and Lang(1990) J. Econ. Entomol. 83 (6): 2480-2485. See, also the experimentalsection herein.

III. Expression Cassettes

Polynucleotides encoding the pesticidal proteins provided herein can beprovided in expression cassettes for expression in an organism ofinterest. The cassette will include 5′ and 3′ regulatory sequencesoperably linked to a polynucleotide encoding a pesticidal polypeptideprovided herein that allows for expression of the polynucleotide. Thecassette may additionally contain at least one additional gene orgenetic element to be cotransformed into the organism. Where additionalgenes or elements are included, the components are operably linked.Alternatively, the additional gene(s) or element(s) can be provided onmultiple expression cassettes. Such an expression cassette is providedwith a plurality of restriction sites and/or recombination sites forinsertion of the polynucleotides to be under the transcriptionalregulation of the regulatory regions. The expression cassette mayadditionally contain a selectable marker gene.

The expression cassette will include in the 5′-3′ direction oftranscription, a transcriptional and translational initiation region(i.e., a promoter), a pesticidal polynucleotide of the invention, and atranscriptional and translational termination region (i.e., terminationregion) functional in the organism of interest, i.e., a plant orbacteria. The promoters of the invention are capable of directing ordriving expression of a coding sequence in a host cell. The regulatoryregions (i.e., promoters, transcriptional regulatory regions, andtranslational termination regions) may be endogenous or heterologous tothe host cell or to each other. As used herein, “heterologous” inreference to a sequence is a sequence that originates from a foreignspecies, or, if from the same species, is substantially modified fromits native form in composition and/or genomic locus by deliberate humanintervention. As used herein, a chimeric gene comprises a codingsequence operably linked to a transcription initiation region that isheterologous to the coding sequence.

Convenient termination regions are available from the Ti-plasmid of A.tumefaciens, such as the octopine synthase and nopaline synthasetermination regions. See also Guerineau et al. (1991) Mol. Gen. Genet.262:141-144; Proudfoot (1991) Cell 64:671-674; Sanfacon et al. (1991)Genes Dev. 5:141-149; Mogen et al. (1990) Plant Cell 2:1261-1272; Munroeet al. (1990) Gene 91:151-158; Ballas et al. (1989) Nucleic Acids Res.17:7891-7903; and Joshi et al. (1987) Nucleic Acids Res. 15:9627-9639.

Additional regulatory signals include, but are not limited to,transcriptional initiation start sites, operators, activators,enhancers, other regulatory elements, ribosomal binding sites, aninitiation codon, termination signals, and the like. See, for example,U.S. Pat. Nos. 5,039,523 and 4,853,331; EPO 0480762A2; Sambrook et al.(1992) Molecular Cloning: A Laboratory Manual, ed. Maniatis et al. (ColdSpring Harbor Laboratory Press, Cold Spring Harbor, N.Y.), hereinafter“Sambrook 11”; Davis et al., eds. (1980) Advanced Bacterial Genetics(Cold Spring Harbor Laboratory Press), Cold Spring Harbor, N.Y., and thereferences cited therein.

In preparing the expression cassette, the various DNA fragments may bemanipulated, so as to provide for the DNA sequences in the properorientation and, as appropriate, in the proper reading frame. Towardthis end, adapters or linkers may be employed to join the DNA fragmentsor other manipulations may be involved to provide for convenientrestriction sites, removal of superfluous DNA, removal of restrictionsites, or the like. For this purpose, in vitro mutagenesis, primerrepair, restriction, annealing, resubstitutions, e.g., transitions andtransversions, may be involved.

A number of promoters can be used in the practice of the invention. Thepromoters can be selected based on the desired outcome. The nucleicacids can be combined with constitutive, inducible, tissue-preferred, orother promoters for expression in the organism of interest. See, forexample, promoters set forth in WO 99/43838 and in U.S. Pat. Nos.8,575,425; 7,790,846; 8,147,856; 8,586832; 7,772,369; 7,534,939;6,072,050; 5,659,026; 5,608,149; 5,608,144; 5,604,121; 5,569,597;5,466,785; 5,399,680; 5,268,463; 5,608,142; and 6,177,611; hereinincorporated by reference.

For expression in plants, constitutive promoters also include CaMV 35Spromoter (Odell et al. (1985) Nature 313:810-812); rice actin (McElroyet al. (1990) Plant Cell 2:163-171); ubiquitin (Christensen et al.(1989) Plant Mol. Biol. 12:619-632 and Christensen et al. (1992) PlantMol. Biol. 18:675-689); pEMU (Last et al. (1991) Theor. Appl. Genet.81:581-588); MAS (Velten et al. (1984) EMBO J. 3:2723-2730). Induciblepromoters include those that drive expression of pathogenesis-relatedproteins (PR proteins), which are induced following infection by apathogen. See, for example, Redolfi et al. (1983) Neth. J. Plant Pathol.89:245-254; Uknes et al. (1992) Plant Cell 4:645-656; and Van Loon(1985) Plant Mol. Virol. 4:111-116; and WO 99/43819, herein incorporatedby reference. Promoters that are expressed locally at or near the siteof pathogen infection may also be used (Marineau et al. (1987) PlantMol. Biol. 9:335-342; Matton et al. (1989) Molecular Plant-MicrobeInteractions 2:325-331; Somsisch et al. (1986) Proc. Natl. Acad. Sci.USA 83:2427-2430; Somsisch et al. (1988) Mol. Gen. Genet. 2:93-98; andYang (1996) Proc. Natl. Acad. Sci. USA 93:14972-14977; Chen et al.(1996) Plant J. 10:955-966; Zhang et al. (1994) Proc. Natl. Acad. Sci.USA 91:2507-2511; Warner et al. (1993) Plant J. 3:191-201; Siebertz etal. (1989) Plant Cell 1:961-968; Cordero et al. (1992) Physiol. Mol.Plant Path. 41:189-200; U.S. Pat. No. 5,750,386 (nematode-inducible);and the references cited therein).

Wound-inducible promoters may be used in the constructions of theinvention. Such wound-inducible promoters include pin II promoter (Ryan(1990) Ann. Rev. Phytopath. 28:425-449; Duan et al. (1996) NatureBiotechnology 14:494-498); wun1 and wun2 (U.S. Pat. No. 5,428,148); win1and win2 (Stanford et al. (1989) Mol. Gen. Genet. 215:200-208); systemin(McGurl et al. (1992) Science 225:1570-1573); WIP1 (Rohmeier et al.(1993) Plant Mol. Biol. 22:783-792; Eckelkamp et al. (1993) FEBS Letters323:73-76); MPI gene (Corderok et al. (1994) Plant J. 6(2):141-150); andthe like, herein incorporated by reference.

Tissue-preferred promoters for use in the invention include those setforth in Yamamoto et al. (1997) Plant J. 12(2):255-265; Kawamata et al.(1997) Plant Cell Physiol. 38(7):792-803; Hansen et al. (1997) Mol. GenGenet. 254(3):337-343; Russell et al. (1997) Transgenic Res.6(2):157-168; Rinehart et al. (1996) Plant Physiol. 112(3):1331-1341;Van Camp et al. (1996) Plant Physiol. 112(2):525-535; Canevascini et al.(1996) Plant Physiol. 112(2):513-524; Yamamoto et al. (1994) Plant CellPhysiol. 35(5):773-778; Lam (1994) Results Probl. Cell Differ.20:181-196; Orozco et al. (1993) Plant Mol Biol. 23(6):1129-1138;Matsuoka et al. (1993) Proc Natl. Acad. Sci. USA 90(20):9586-9590; andGuevara-Garcia et al. (1993) Plant J. 4(3):495-505.

Leaf-preferred promoters include those set forth in Yamamoto et al.(1997) Plant J. 12(2):255-265; Kwon et al. (1994) Plant Physiol.105:357-67; Yamamoto et al. (1994) Plant Cell Physiol. 35(5):773-778;Gotor et al. (1993) Plant J. 3:509-18; Orozco et al. (1993) Plant Mol.Biol. 23(6):1129-1138; and Matsuoka et al. (1993) Proc. Natl. Acad. Sci.USA 90(20):9586-9590.

Root-preferred promoters are known and include those in Hire et al.(1992) Plant Mol. Biol. 20(2):207-218 (soybean root-specific glutaminesynthetase gene); Keller and Baumgartner (1991) Plant Cell3(10):1051-1061 (root-specific control element); Sanger et al. (1990)Plant Mol. Biol. 14(3):433-443 (mannopine synthase (MAS) gene ofAgrobacterium tumefaciens); and Miao et al. (1991) Plant Cell 3(1):11-22(cytosolic glutamine synthetase (GS)); Bogusz et al. (1990) Plant Cell2(7):633-641; Leach and Aoyagi (1991) Plant Science (Limerick)79(1):69-76 (rolC and rolD); Teeri et al. (1989) EMBO J. 8(2):343-350;Kuster et al. (1995) Plant Mol. Biol. 29(4):759-772 (the VfENOD-GRP3gene promoter); and, Capana et al. (1994) Plant Mol. Biol. 25(4):681-691(rolB promoter). See also U.S. Pat. Nos. 5,837,876; 5,750,386;5,633,363; 5,459,252; 5,401,836; 5,110,732; and 5,023,179.

“Seed-preferred” promoters include both “seed-specific” promoters (thosepromoters active during seed development such as promoters of seedstorage proteins) as well as “seed-germinating” promoters (thosepromoters active during seed germination). See Thompson et al. (1989)BioEssays 10:108. Seed-preferred promoters include, but are not limitedto, Cim1 (cytokinin-induced message); cZ19B1 (maize 19 kDa zein); mi1ps(myo-inositol-1-phosphate synthase) (see WO 00/11177 and U.S. Pat. No.6,225,529). Gamma-zein is an endosperm-specific promoter. Globulin 1(Glb-1) is a representative embryo-specific promoter. For dicots,seed-specific promoters include, but are not limited to, beanβ-phaseolin, napin, β-conglycinin, soybean lectin, cruciferin, and thelike. For monocots, seed-specific promoters include, but are not limitedto, maize 15 kDa zein, 22 kDa zein, 27 kDa zein, gamma-zein, waxy,shrunken 1, shrunken 2, Globulin 1, etc. See also WO 00/12733, whereseed-preferred promoters from end1 and end2 genes are disclosed.

For expression in a bacterial host, promoters that function in bacteriaare well-known in the art. Such promoters include any of the knowncrystal protein gene promoters, including the promoters of any of thepesticidal proteins of the invention, and promoters specific for B.thuringiensis sigma factors. Alternatively, mutagenized or recombinantcrystal protein-encoding gene promoters may be recombinantly engineeredand used to promote expression of the novel gene segments disclosedherein.

The expression cassette can also comprise a selectable marker gene forthe selection of transformed cells. Selectable marker genes are utilizedfor the selection of transformed cells or tissues. Marker genes includegenes encoding antibiotic resistance, such as those encoding neomycinphosphotransferase II (NEO) and hygromycin phosphotransferase (HPT), aswell as genes conferring resistance to herbicidal compounds, such asglufosinate ammonium, bromoxynil, imidazolinones, and2,4-dichlorophenoxyacetate (2,4-D). Additional selectable markers areknown and any can be used in the practice of the invention. See, forexample, PCT/US2015/066648, filed on Dec. 18, 2015, herein incorporatedby reference in its entirety, which discloses glufosinate resistancesequences that can be employed as selectable markers.

IV. Methods, Host Cells and Plant Cells

As indicated, DNA constructs comprising nucleotide sequences encodingthe pesticidal proteins or active variants or fragment thereof can beused to transform plants of interest or other organisms of interest.Methods for transformation involve introducing a nucleotide constructinto a plant. By “introducing” is intended to introduce the nucleotideconstruct to the plant or other host cell in such a manner that theconstruct gains access to the interior of a cell of the plant or hostcell. The methods of the invention do not require a particular methodfor introducing a nucleotide construct to a plant or host cell, onlythat the nucleotide construct gains access to the interior of at leastone cell of the plant or the host organism. Methods for introducingnucleotide constructs into plants and other host cells are known in theart including, but not limited to, stable transformation methods,transient transformation methods, and virus-mediated methods.

The methods result in a transformed organisms, such as a plant,including whole plants, as well as plant organs (e.g., leaves, stems,roots, etc.), seeds, plant cells, propagules, embryos and progeny of thesame. Plant cells can be differentiated or undifferentiated (e.g.callus, suspension culture cells, protoplasts, leaf cells, root cells,phloem cells, pollen).

“Transgenic plants” or “transformed plants” or “stably transformed”plants or cells or tissues refers to plants that have incorporated orintegrated a polynucleotide encoding at least one pesticidal polypeptideof the invention. It is recognized that other exogenous or endogenousnucleic acid sequences or DNA fragments may also be incorporated intothe plant cell. Agrobacterium- and biolistic-mediated transformationremain the two predominantly employed approaches. However,transformation may be performed by infection, transfection,microinjection, electroporation, microprojection, biolistics or particlebombardment, electroporation, silica/carbon fibers, ultrasound mediated,PEG mediated, calcium phosphate co-precipitation, polycation DMSOtechnique, DEAE dextran procedure, Agro and viral mediated(Caulimoriviruses, Geminiviruses, RNA plant viruses), liposome mediatedand the like.

Transformation protocols as well as protocols for introducingpolypeptides or polynucleotide sequences into plants may vary dependingon the type of plant or plant cell, i.e., monocot or dicot, targeted fortransformation. Methods for transformation are known in the art andinclude those set forth in U.S. Pat. Nos. 8,575,425; 7,692,068;8,802,934; 7,541,517; each of which is herein incorporated by reference.See, also, Rakoczy-Trojanowska, M. (2002) Cell Mol Biol Lett. 7:849-858;Jones et al. (2005) Plant Methods 1:5; Rivera et al. (2012) Physics ofLife Reviews 9:308-345; Bartlett et al. (2008) Plant Methods 4:1-12;Bates, G. W. (1999) Methods in Molecular Biology 111:359-366; Binns andThomashow (1988) Annual Reviews in Microbiology 42:575-606; Christou, P.(1992) The Plant Journal 2:275-281; Christou, P. (1995) Euphytica85:13-27; Tzfira et al. (2004) TRENDS in Genetics 20:375-383; Yao et al.(2006) Journal of Experimental Botany 57:3737-3746; Zupan and Zambryski(1995) Plant Physiology 107:1041-1047; Jones et al. (2005) Plant Methods1:5.

Transformation may result in stable or transient incorporation of thenucleic acid into the cell. “Stable transformation” is intended to meanthat the nucleotide construct introduced into a host cell integratesinto the genome of the host cell and is capable of being inherited bythe progeny thereof “Transient transformation” is intended to mean thata polynucleotide is introduced into the host cell and does not integrateinto the genome of the host cell.

Methods for transformation of chloroplasts are known in the art. See,for example, Svab et al. (1990) Proc. Nail. Acad. Sci. USA 87:8526-8530;Svab and Maliga (1993) Proc. Natl. Acad. Sci. USA 90:913-917; Svab andMaliga (1993) EMBO J. 12:601-606. The method relies on particle gundelivery of DNA containing a selectable marker and targeting of the DNAto the plastid genome through homologous recombination. Additionally,plastid transformation can be accomplished by transactivation of asilent plastid-borne transgene by tissue-preferred expression of anuclear-encoded and plastid-directed RNA polymerase. Such a system hasbeen reported in McBride et al. (1994) Proc. Natl. Acad. Sci. USA91:7301-7305.

The cells that have been transformed may be grown into plants inaccordance with conventional ways. See, for example, McCormick et al.(1986) Plant Cell Reports 5:81-84. These plants may then be grown, andeither pollinated with the same transformed strain or different strains,and the resulting hybrid having constitutive expression of the desiredphenotypic characteristic identified. Two or more generations may begrown to ensure that expression of the desired phenotypic characteristicis stably maintained and inherited and then seeds harvested to ensureexpression of the desired phenotypic characteristic has been achieved.In this manner, the present invention provides transformed seed (alsoreferred to as “transgenic seed”) having a nucleotide construct of theinvention, for example, an expression cassette of the invention, stablyincorporated into their genome.

In specific embodiments, the sequences provide herein can be targeted tospecific sites within the genome of the host cell or plant cell. Suchmethods include, but are not limited to, meganucleases designed againstthe plant genomic sequence of interest (D'Halluin et al. 2013 PlantBiotechnol J); CRISPR-Cas9, TALENs, and other technologies for preciseediting of genomes (Feng, et al. Cell Research 23:1229-1232, 2013,Podevin, et al. Trends Biotechnology, online publication, 2013, Wei etal., J. Gen Genomics, 2013, Zhang et al (2013) WO 2013/026740); Cre-loxsite-specific recombination (Dale et al. (1995) Plant J 7:649-659;Lyznik, et al. (2007) Transgenic Plant J 1:1-9; FLP-FRT recombination(Li et al. (2009) Plant Physiol 151:1087-1095); Bxb1-mediatedintegration (Yau et al. Plant J (2011) 701:147-166); zinc-fingermediated integration (Wright et al. (2005) Plant J 44:693-705); Cai etal. (2009) Plant Mol Biol 69:699-709); and homologous recombination(Lieberman-Lazarovich and Levy (2011) Methods Mol Biol 701: 51-65);Puchta (2002) Plant Mol Biol 48:173-182).

The sequence provided herein may be used for transformation of any plantspecies, including, but not limited to, monocots and dicots. Examples ofplants of interest include, but are not limited to, corn (maize),sorghum, wheat, sunflower, tomato, crucifers, peppers, potato, cotton,rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape,Brassica sp., alfalfa, rye, millet, safflower, peanuts, sweet potato,cassaya, coffee, coconut, pineapple, citrus trees, cocoa, tea, banana,avocado, fig, guava, mango, olive, papaya, cashew, macadamia, almond,oats, vegetables, ornamentals, and conifers.

Vegetables include, but are not limited to, tomatoes, lettuce, greenbeans, lima beans, peas, and members of the genus Curcumis such ascucumber, cantaloupe, and musk melon. Ornamentals include, but are notlimited to, azalea, hydrangea, hibiscus, roses, tulips, daffodils,petunias, carnation, poinsettia, and chrysanthemum. Preferably, plantsof the present invention are crop plants (for example, maize, sorghum,wheat, sunflower, tomato, crucifers, peppers, potato, cotton, rice,soybean, sugarbeet, sugarcane, tobacco, barley, oilseed rape, etc.).

As used herein, the term plant includes plant cells, plant protoplasts,plant cell tissue cultures from which plants can be regenerated, plantcalli, plant clumps, and plant cells that are intact in plants or partsof plants such as embryos, pollen, ovules, seeds, leaves, flowers,branches, fruit, kernels, ears, cobs, husks, stalks, roots, root tips,anthers, and the like. Grain is intended to mean the mature seedproduced by commercial growers for purposes other than growing orreproducing the species. Progeny, variants, and mutants of theregenerated plants are also included within the scope of the invention,provided that these parts comprise the introduced polynucleotides.Further provided is a processed plant product or byproduct that retainsthe sequences disclosed herein, including for example, soymeal.

In another embodiment, the genes encoding the pesticidal proteins can beused to transform insect pathogenic organisms. Such organisms includebaculoviruses, fungi, protozoa, bacteria, and nematodes. Microorganismhosts that are known to occupy the “phytosphere” (phylloplane,phyllosphere, rhizosphere, and/or rhizoplana) of one or more crops ofinterest may be selected. These microorganisms are selected so as to becapable of successfully competing in the particular environment with thewild-type microorganisms, provide for stable maintenance and expressionof the gene expressing the pesticidal protein, and desirably, providefor improved protection of the pesticide from environmental degradationand inactivation.

Such microorganisms include archaea, bacteria, algae, and fungi. Ofparticular interest are microorganisms such as bacteria, e.g., Bacillus,Pseudomonas, Erwinia, Serratia, Klebsiella, Xanthomonas, Streptomyces,Rhizobium, Rhodopseudomonas, Methylius, Agrobacterium, Acetobacter,Lactobacillus, Arthrobacter, Azotobacter, Leuconostoc, and Alcaligenes.Fungi include yeast, e.g., Saccharomyces, Cryptococcus, Kluyveromyces,Sporobolomyces, Rhodotorula, and Aureobasidium. Of particular interestare such phytosphere bacterial species as Pseudomonas syringae,Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens,Acetobacter xylinum, Agrobacteria, Rhodopseudomonas spheroides,Xanthomonas campestris, Rhizobium melioti, Alcaligenes entrophus,Clavibacter xyli and Azotobacter vinlandir and phytosphere yeast speciessuch as Rhodotorula rubra, R. glutinis, R. marina, R. aurantiaca,Cryptococcus albidus, C. diffluens, C. laurentii, Saccharomyces rosei,S. pretoriensis, S. cerevisiae, Sporobolomyces rosues, S. odorus,Kluyveromyces veronae, Aureobasidium pollulans, Bacillus thuringiensis,Escherichia coli, Bacillus subtilis, and the like.

Illustrative prokaryotes, both Gram-negative and gram-positive, includeEnterobacteriaceae, such as Escherichia, Erwinia, Shigella, Salmonella,and Proteus; Bacillaceae; Rhizobiceae, such as Rhizobium; Spirillaceae,such as photobacterium, Zymomonas, Serratia, Aeromonas, Vibrio,Desulfovibrio, Spirillum; Lactobacillaceae; Pseudomonadaceae, such asPseudomonas and Acetobacter; Azotobacteraceae and Nitrobacteraceae.Fungi include Phycomycetes and Ascomycetes, e.g., yeast, such asSaccharomyces and Schizosaccharomyces; and Basidiomycetes yeast, such asRhodotorula, Aureobasidium, Sporobolomyces, and the like.

Genes encoding pesticidal proteins can be introduced by means ofelectrotransformation, PEG induced transformation, heat shock,transduction, conjugation, and the like. Specifically, genes encodingthe pesticidal proteins can be cloned into a shuttle vector, forexample, pHT3101 (Lerecius et al. (1989) FEMS Microbiol. Letts. 60:211-218. The shuttle vector pHT3101 containing the coding sequence forthe particular pesticidal protein gene can, for example, be transformedinto the root-colonizing Bacillus by means of electroporation (Lereciuset al. (1989) FEMS Microbiol. Letts. 60: 211-218).

Expression systems can be designed so that pesticidal proteins aresecreted outside the cytoplasm of gram-negative bacteria by fusing anappropriate signal peptide to the amino-terminal end of the pesticidalprotein. Signal peptides recognized by E. coli include the OmpA protein(Ghrayeb et al. (1984) EMBO J, 3: 2437-2442).

Pesticidal proteins and active variants thereof can be fermented in abacterial host and the resulting bacteria processed and used as amicrobial spray in the same manner that Bacillus thuringiensis strainshave been used as insecticidal sprays. In the case of a pesticidalprotein(s) that is secreted from Bacillus, the secretion signal isremoved or mutated using procedures known in the art. Such mutationsand/or deletions prevent secretion of the pesticidal protein(s) into thegrowth medium during the fermentation process. The pesticidal proteinsare retained within the cell, and the cells are then processed to yieldthe encapsulated pesticidal proteins.

Alternatively, the pesticidal proteins are produced by introducingheterologous genes into a cellular host. Expression of the heterologousgene results, directly or indirectly, in the intracellular productionand maintenance of the pesticide. These cells are then treated underconditions that prolong the activity of the toxin produced in the cellwhen the cell is applied to the environment of target pest(s). Theresulting product retains the toxicity of the toxin. These naturallyencapsulated pesticidal proteins may then be formulated in accordancewith conventional techniques for application to the environment hostinga target pest, e.g., soil, water, and foliage of plants. See, forexample U.S. Pat. No. 6,468,523 and U.S. Publication No. 20050138685,and the references cited therein. In the present invention, atransformed microorganism (which includes whole organisms, cells,spore(s), pesticidal protein(s), pesticidal component(s), pest-impactingcomponent(s), mutant(s), living or dead cells and cell components,including mixtures of living and dead cells and cell components, andincluding broken cells and cell components) or an isolated pesticidalprotein can be formulated with an acceptable carrier into a pesticidalor agricultural composition(s) that is, for example, a suspension, asolution, an emulsion, a dusting powder, a dispersible granule, awettable powder, and an emulsifiable concentrate, an aerosol, animpregnated granule, an adjuvant, a coatable paste, and alsoencapsulations in, for example, polymer substances.

Agricultural compositions may comprise a polypeptide, a recombinogenicpolypeptide or a variant or fragment thereof, as disclosed herein. Theagricultural composition disclosed herein may be applied to theenvironment of a plant or an area of cultivation, or applied to theplant, plant part, plant cell, or seed.

Such compositions disclosed above may be obtained by the addition of asurface-active agent, an inert carrier, a preservative, a humectant, afeeding stimulant, an attractant, an encapsulating agent, a binder, anemulsifier, a dye, a UV protectant, a buffer, a flow agent orfertilizers, micronutrient donors, or other preparations that influenceplant growth. One or more agrochemicals including, but not limited to,herbicides, insecticides, fungicides, bactericides, nematicides,molluscicides, acaracides, plant growth regulators, harvest aids, andfertilizers, can be combined with carriers, surfactants or adjuvantscustomarily employed in the art of formulation or other components tofacilitate product handling and application for particular target pests.Suitable carriers and adjuvants can be solid or liquid and correspond tothe substances ordinarily employed in formulation technology, e.g.,natural or regenerated mineral substances, solvents, dispersants,wetting agents, tackifiers, binders, or fertilizers. The activeingredients of the present invention are normally applied in the form ofcompositions and can be applied to the crop area, plant, or seed to betreated. For example, the compositions of the present invention may beapplied to grain in preparation for or during storage in a grain bin orsilo, etc. The compositions of the present invention may be appliedsimultaneously or in succession with other compounds. Methods ofapplying an active ingredient of the present invention or anagrochemical composition of the present invention that contains at leastone of the pesticidal proteins produced by the bacterial strains of thepresent invention include, but are not limited to, foliar application,seed coating, and soil application. The number of applications and therate of application depend on the intensity of infestation by thecorresponding pest.

Suitable surface-active agents include, but are not limited to, anioniccompounds such as a carboxylate of, for example, a metal; a carboxylateof a long chain fatty acid; an N-acylsarcosinate; mono or di-esters ofphosphoric acid with fatty alcohol ethoxylates or salts of such esters;fatty alcohol sulfates such as sodium dodecyl sulfate, sodium octadecylsulfate or sodium cetyl sulfate; ethoxylated fatty alcohol sulfates;ethoxylated alkylphenol sulfates; lignin sulfonates; petroleumsulfonates; alkyl aryl sulfonates such as alkyl-benzene sulfonates orlower alkylnaphtalene sulfonates, e.g., butyl-naphthalene sulfonate;salts of sulfonated naphthalene-formaldehyde condensates; salts ofsulfonated phenol-formaldehyde condensates; more complex sulfonates suchas the amide sulfonates, e.g., the sulfonated condensation product ofoleic acid and N-methyl taurine; or the dialkyl sulfosuccinates, e.g.,the sodium sulfonate of dioctyl succinate. Non-ionic agents includecondensation products of fatty acid esters, fatty alcohols, fatty acidamides or fatty-alkyl- or alkenyl-substituted phenols with ethyleneoxide, fatty esters of polyhydric alcohol ethers, e.g., sorbitan fattyacid esters, condensation products of such esters with ethylene oxide,e.g., polyoxyethylene sorbitar fatty acid esters, block copolymers ofethylene oxide and propylene oxide, acetylenic glycols such as2,4,7,9-tetraethyl-5-decyn-4,7-diol, or ethoxylated acetylenic glycols.Examples of a cationic surface-active agent include, for instance, analiphatic mono-, di-, or polyamine such as an acetate, naphthenate oroleate; or oxygen-containing amine such as an amine oxide ofpolyoxyethylene alkylamine; an amide-linked amine prepared by thecondensation of a carboxylic acid with a di- or polyamine; or aquaternary ammonium salt.

Examples of inert materials include but are not limited to inorganicminerals such as kaolin, phyllosilicates, carbonates, sulfates,phosphates, or botanical materials such as cork, powdered corncobs,peanut hulls, rice hulls, and walnut shells.

The compositions of the present invention can be in a suitable form fordirect application or as a concentrate of primary composition thatrequires dilution with a suitable quantity of water or other diluantbefore application. The pesticidal concentration will vary dependingupon the nature of the particular formulation, specifically, whether itis a concentrate or to be used directly. The composition contains 1 to98% of a solid or liquid inert carrier, and 0 to 50% or 0.1 to 50% of asurfactant. These compositions will be administered at the labeled ratefor the commercial product, for example, about 0.01 lb-5.0 lb. per acrewhen in dry form and at about 0.01 pts.-10 pts. per acre when in liquidform.

In a further embodiment, the compositions, as well as the transformedmicroorganisms and pesticidal proteins, provided herein can be treatedprior to formulation to prolong the pesticidal activity when applied tothe environment of a target pest as long as the pretreatment is notdeleterious to the pesticidal activity. Such treatment can be bychemical and/or physical means as long as the treatment does notdeleteriously affect the properties of the composition(s). Examples ofchemical reagents include but are not limited to halogenating agents;aldehydes such as formaldehyde and glutaraldehyde; anti-infectives, suchas zephiran chloride; alcohols, such as isopropanol and ethanol; andhistological fixatives, such as Bouin's fixative and Helly's fixative(see, for example, Humason (1967) Animal Tissue Techniques (W.H. Freemanand Co.).

In one aspect, pests may be killed or reduced in numbers in a given areaby application of the pesticidal proteins of the invention to the area.Alternatively, the pesticidal proteins may be prophylactically appliedto an environmental area to prevent infestation by a susceptible pest.Preferably the pest ingests, or is contacted with, apesticidally-effective amount of the polypeptide. By“pesticidally-effective amount” is intended an amount of the pesticidethat is able to bring about death to at least one pest, or to noticeablyreduce pest growth, feeding, or normal physiological development. Thisamount will vary depending on such factors as, for example, the specifictarget pests to be controlled, the specific environment, location,plant, crop, or agricultural site to be treated, the environmentalconditions, and the method, rate, concentration, stability, and quantityof application of the pesticidally-effective polypeptide composition.The formulations or compositions may also vary with respect to climaticconditions, environmental considerations, and/or frequency ofapplication and/or severity of pest infestation.

The active ingredients are normally applied in the form of compositionsand can be applied to the crop area, plant, or seed to be treated.Methods are therefore provided for providing to a plant, plant cell,seed, plant part or an area of cultivation, an effective amount of theagricultural composition comprising the polypeptide, recombinogenicpolypeptide or an active variant or fragment thereof. By “effectiveamount” is intended an amount of a protein or composition sufficient tokill or control the pest or result in a noticeable reduction in pestgrowth, feeding, or normal physiological development. Such decreases inpest numbers, pest growth, pest feeding or pest normal development cancomprise any statistically significant decrease, including, for examplea decrease of about 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%,55%, 60%, 65%, 70%, 75%, 85%, 90%, 95% or greater. For example, thecompositions may be applied to grain in preparation for or duringstorage in a grain bin or silo, etc. The compositions may be appliedsimultaneously or in succession with other compounds. Methods ofapplying an active ingredient or an agrochemical composition comprisingat least one of the polypeptides, recombinogenic polypeptides orvariants or fragments thereof as disclosed herein, include but are notlimited to, foliar application, seed coating, and soil application.

Methods for increasing plant yield are provided. The methods compriseproviding a plant or plant cell expressing a polynucleotide encoding thepesticidal polypeptide sequence disclosed herein and growing the plantor a seed thereof in a field infested with (or susceptible toinfestation by) a pest against which said polypeptide has pesticidalactivity. In some embodiments, the polypeptide has pesticidal activityagainst a lepidopteran, coleopteran, dipteran, hemipteran, or nematodepest, and said field is infested with a lepidopteran, hemipteran,coleopteran, dipteran, or nematode pest. As defined herein, the “yield”of the plant refers to the quality and/or quantity of biomass producedby the plant. By “biomass” is intended any measured plant product. Anincrease in biomass production is any improvement in the yield of themeasured plant product. Increasing plant yield has several commercialapplications. For example, increasing plant leaf biomass may increasethe yield of leafy vegetables for human or animal consumption.Additionally, increasing leaf biomass can be used to increase productionof plant-derived pharmaceutical or industrial products. An increase inyield can comprise any statistically significant increase including, butnot limited to, at least a 1% increase, at least a 3% increase, at leasta 5% increase, at least a 10% increase, at least a 20% increase, atleast a 30%, at least a 50%, at least a 70%, at least a 100% or agreater increase in yield compared to a plant not expressing thepesticidal sequence. In specific methods, plant yield is increased as aresult of improved pest resistance of a plant expressing a pesticidalprotein disclosed herein. Expression of the pesticidal protein resultsin a reduced ability of a pest to infest or feed.

The plants can also be treated with one or more chemical compositions,including one or more herbicide, insecticides, or fungicides.

Non-limiting embodiments include:

1. An isolated polypeptide having insecticidal activity, comprising:

-   -   (a) a polypeptide comprising an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID        NOs:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,        18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,        34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49,        50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,        66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,        82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,        98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,        111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123,        124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,        137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149,        150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162,        163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,        176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,        189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201,        202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214,        215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,        228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,        241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,        254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,        267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279,        280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,        293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305,        306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,        319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331,        332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344,        345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,        358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,        371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383,        384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396,        397, or 398; or    -   (b) a polypeptide comprising an amino acid sequence having at        least the percent sequence identity set forth in Table 1 to an        amino acid sequence selected from the group consisting of        sequences set forth in SEQ ID Nos: 1, 2, 3, 4, 5, 6, 7, 8, 9,        10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,        26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41,        42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57,        58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73,        74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89,        90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,        105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117,        118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130,        131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,        144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154, 155, 156,        157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,        170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,        183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195,        196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208,        209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221,        222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234,        235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247,        248, 249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260,        261, 262, 263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273,        274, 275, 276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286,        287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297, 298, 299,        300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311, 312,        313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,        326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,        339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351,        352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,        365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377,        378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390,        391, 392, 393, 394, 395, 396, 397, or 398.

2. The polypeptide of embodiment 1, wherein said polypeptide comprisesthe amino acid sequence set forth in SEQ ID Nos. 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153, 154,155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168,169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182,183, 184, 185, 186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196,197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210,211, 212, 213, 214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224,225, 226, 227, 228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238,239, 240, 241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252,253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280,281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294,295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308,309, 310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336,337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350,351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364,365, 366, 367, 368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378,379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392,393, 394, 395, 396, 397, or 398.

3. The polypeptide of embodiment 1 or 2, further comprising heterologousamino acid sequences.

4. A composition comprising the polypeptide of embodiments 1, 2, or 3.

5. A recombinant nucleic acid molecule that encodes the polypeptide ofany one of embodiments 1 to 3, wherein said recombinant nucleic acidmolecule is not the naturally occurring sequence encoding saidpolypeptide.

6. The recombinant nucleic acid of embodiment 5, wherein said nucleicacid molecule is a synthetic sequence that has been designed forexpression in a plant.

7. The recombinant nucleic acid molecule of embodiment 5 or 6, whereinsaid nucleic acid molecule is operably linked to a promoter capable ofdirecting expression in a plant cell.

8. The recombinant nucleic acid molecule of any one of embodiments 5 to7, wherein said nucleic acid molecule is operably linked to a promotercapable of directing expression in a bacteria.

9. A host cell that contains the recombinant nucleic acid molecule ofany one of embodiments 5 to 8.

10. The host cell of embodiment 9, wherein said host cell is a bacterialhost cell.

11. A DNA construct comprising a promoter that drives expression in aplant cell operably linked to a recombinant nucleic acid moleculecomprising:

-   -   (a) a nucleotide sequence that encodes a polypeptide comprising        the amino acid sequence of any one of SEQ ID NOs:1, 2, 3, 4, 5,        6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,        23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,        39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,        55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70,        71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,        87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,        102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,        115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,        128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,        141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153,        154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,        167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,        180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,        193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205,        206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218,        219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,        232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,        245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,        258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270,        271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,        284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,        297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,        310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,        323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,        336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,        349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,        362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,        375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,        388, 389, 390, 391, 392, 393, 394, 395, 396, 397, or 398; or,    -   (b) a nucleotide sequence that encodes a polypeptide comprising        an amino acid sequence having at least the percent sequence        identity set forth in Table 1 to an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID        NOs:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,        18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,        34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49,        50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,        66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,        82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,        98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,        111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123,        124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,        137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149,        150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162,        163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,        176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,        189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201,        202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214,        215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,        228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,        241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,        254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,        267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279,        280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,        293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305,        306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,        319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331,        332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344,        345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,        358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,        371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383,        384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396,        397, or 398.

12. The DNA construct of embodiment 11, wherein said nucleotide sequenceis a synthetic DNA sequence that has been designed for expression in aplant.

13. A vector comprising the DNA construct of embodiment 11 or 12.

14. A host cell that contains the DNA construct of any one ofembodiments 11-13 or the vector of embodiment 13.

15. The host cell of embodiment 14, wherein the host cell is a plantcell.

16. A transgenic plant comprising the host cell of embodiment 15.

17. A composition comprising the host cell of any one of embodiments 9,10, 14, or 15.

18. The composition of embodiment 17, wherein said composition isselected from the group consisting of a powder, dust, pellet, granule,spray, emulsion, colloid, and solution.

19. The composition of embodiment 17 or 18, wherein said compositioncomprises from about 1% to about 99% by weight of said polypeptide.

20. A method for controlling a pest population comprising contactingsaid population with a pesticidal-effective amount of the composition ofany one of embodiments 4 or 17-19.

21. A method for killing a pest population comprising contacting saidpopulation with a pesticidal-effective amount of the composition of anyone of embodiments 4 or 17-19.

22. A method for producing a polypeptide with pesticidal activity,comprising culturing the host cell of any one of embodiments 9, 10, 14,or 15 under conditions in which the nucleic acid molecule encoding thepolypeptide is expressed.

23. A plant having stably incorporated into its genome a DNA constructcomprising a nucleotide sequence that encodes a protein havingpesticidal activity, wherein said nucleotide sequence comprise:

-   -   (a) a nucleotide sequence that encodes a polypeptide comprising        the amino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5,        6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,        23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,        39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,        55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70,        71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,        87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,        102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,        115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,        128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,        141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153,        154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,        167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,        180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,        193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205,        206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218,        219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,        232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,        245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,        258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270,        271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,        284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,        297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,        310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,        323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,        336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,        349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,        362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,        375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,        388, 389, 390, 391, 392, 393, 394, 395, 396, 397, or 398; or,    -   (b) a nucleotide sequence that encodes a polypeptide comprising        an amino acid sequence having at least the percent sequence        identity set forth in Table 1 to an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID        NOs:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,        18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,        34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49,        50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,        66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,        82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,        98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,        111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123,        124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,        137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149,        150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162,        163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,        176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,        189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201,        202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214,        215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,        228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,        241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,        254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,        267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279,        280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,        293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305,        306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,        319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331,        332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344,        345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,        358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,        371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383,        384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396,        397, or 398.

24. A transgenic seed of the plant of embodiment 23.

25. A method for protecting a plant from an insect pest, comprisingexpressing in a plant or cell thereof a nucleotide sequence that encodesa pesticidal polypeptide, wherein said nucleotide sequence comprising:

-   -   (a) a nucleotide sequence that encodes a polypeptide comprising        the amino acid sequence of any one of SEQ ID NOs: 1, 2, 3, 4, 5,        6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,        23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,        39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,        55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70,        71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,        87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,        102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,        115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,        128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,        141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153,        154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,        167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,        180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,        193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205,        206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218,        219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,        232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,        245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,        258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270,        271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,        284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,        297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,        310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,        323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,        336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,        349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,        362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,        375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,        388, 389, 390, 391, 392, 393, 394, 395, 396, 397, or 398; or,    -   (b) a nucleotide sequence that encodes a polypeptide comprising        an amino acid sequence having at least the percent sequence        identity set forth in Table 1 to an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID        NOs:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,        18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,        34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49,        50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,        66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,        82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,        98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,        111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123,        124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,        137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149,        150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162,        163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,        176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,        189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201,        202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214,        215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,        228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,        241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,        254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,        267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279,        280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,        293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305,        306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,        319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331,        332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344,        345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,        358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,        371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383,        384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396,        397, or 398.

26. The method of embodiment 25, wherein said plant produces apesticidal polypeptide having pesticidal activity against a lepidopteranor coleopteran pest.

27. A method for increasing yield in a plant comprising growing in afield a plant or seed thereof having stably incorporated into its genomea DNA construct comprising a promoter that drives expression in a plantoperably linked to a nucleotide sequence that encodes a pesticidalpolypeptide, wherein said nucleotide sequence comprises:

-   -   (a) a nucleotide sequence that encodes a polypeptide comprising        the amino acid sequence of any one of SEQ ID NOs:1, 2, 3, 4, 5,        6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22,        23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38,        39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,        55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70,        71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,        87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,        102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114,        115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127,        128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,        141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152, 153,        154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166,        167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179,        180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,        193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205,        206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218,        219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,        232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244,        245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255, 256, 257,        258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269, 270,        271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,        284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296,        297, 298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309,        310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322,        323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335,        336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348,        349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,        362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,        375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,        388, 389, 390, 391, 392, 393, 394, 395, 396, 397, or 398; or,    -   (b) a nucleotide sequence that encodes a polypeptide comprising        an amino acid sequence having at least the percent sequence        identity set forth in Table 1 to an amino acid sequence selected        from the group consisting of sequences set forth in SEQ ID        NOs:1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17,        18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33,        34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49,        50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65,        66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81,        82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,        98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110,        111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123,        124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136,        137, 138, 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149,        150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162,        163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,        176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188,        189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201,        202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214,        215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,        228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240,        241, 242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253,        254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266,        267, 268, 269, 270, 271, 272, 273, 274, 275, 276, 277, 278, 279,        280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290, 291, 292,        293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304, 305,        306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,        319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331,        332, 333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344,        345, 346, 347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357,        358, 359, 360, 361, 362, 363, 364, 365, 366, 367, 368, 369, 370,        371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383,        384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396,        397, or 398.

28. A method of obtaining a polynucleotide that encodes an improvedpolypeptide comprising pesticidal activity is provided, wherein theimproved polypeptide has at least one improved property over any one ofSEQ ID NOS: 1-398 comprising:

-   -   (a) recombining a plurality of parental polynucleotides        comprising SEQ ID NO: 1-398 or an active variant or fragment        thereof to produce a library of recombinant polynucleotides        encoding recombinant pesticidal polypeptides;    -   (b) screening the library to identify a recombinant        polynucleotide that encodes an improved recombinant pesticidal        polypeptide that has an enhanced property improved over the        parental polynucleotide;    -   (c) recovering the recombinant polynucleotide that encodes the        improved recombinant pesticidal polypeptide identified in (b);        and,    -   (d) repeating steps (a), (b) and (c) using the recombinant        polynucleotide recovered in step (c) as one of the plurality of        parental polynucleotides in repeated step (a).

The following examples are offered by way of illustration and not by wayof limitation.

EXPERIMENTAL Experiment 1: Discovery of Novel Genes by Sequencing andDNA Analysis

Microbial cultures were grown in liquid culture in standard laboratorymedia. Cultures were grown to saturation (16 to 24 hours) before DNApreparation. DNA was extracted from bacterial cells by detergent lysis,followed by binding to a silica matrix and washing with an ethanolbuffer. Purified DNA was eluted from the silica matrix with a mildlyalkaline aqueous buffer.

DNA for sequencing was tested for purity and concentration byspectrophotometry. Sequencing libraries were prepared using the NexteraXT library preparation kit according to the manufacturer's protocol.Sequence data was generated on a HiSeq 2000 according to the IlluminaHiSeq 2000 System User Guide protocol.

Sequencing reads were assembled into draft genomes using the CLC BioAssembly Cell software package. Following assembly, gene calls were madeby several methods and resulting gene sequences were interrogated toidentify novel homologs of pesticidal genes. Novel genes were identifiedby BLAST, by domain composition, and by pairwise alignment versus atarget set of pesticidal genes. A summary of such sequences is set forthin Table 1.

Genes identified in the homology search were amplified from bacterialDNA by PCR and cloned into bacterial expression vectors containing fusedin-frame purification tags. Cloned genes were expressed in E. coli andpurified by column chromatography. Purified proteins were assessed ininsect diet bioassay studies to identify active proteins.

Insect diet bioassays were performed using a wheat germ and agarartificial diet to which purified protein were applied as a surfacetreatment. Insect larvae were applied to treated diet and monitored formortality.

Insect diet bioassays were performed using a sucrose liquid dietcontained in a membrane sachet to which purified protein was added.Insect nymphs were allowed to feed on the diet sachet and were monitoredfor mortality. Insects tested in bioassays included the Brown Stink Bug(BSB), Euschistus servus, and the Southern Green Stink Bug (SGSB),Nezara viridula.

Example 2. Heterologous Expression in E. coli

Each open reading frame set forth in Tables 3 and 4 was cloned into anE. coli expression vector containing a maltose binding protein (pMBP).The expression vector was transformed into BL21*RIPL. An LB culturesupplemented with carbenicillin was inoculated with a single colony andgrown overnight at 37° C. using 0.5% of the overnight culture, a freshculture was inoculated and grown to logarithmic phase at 37° C. Theculture was induced using 250 mM IPTG for 18 hours at 16° C. The cellswere pelleted and resuspended in 10 mM Tris pH7.4 and 150 mM NaClsupplemented with protease inhibitors. The protein expression wasevaluated by SDS-PAGE.

Example 3. Pesticidal Activity Against Coleopteran and Lepidoptera

Protein Expression: Each sequence set forth in Table 3 was expressed inE. coli as described in Example 2. 400 mL of LB was inoculated and grownto an OD600 of 0.6. The culture was induced with 0.25 mM IPTG overnightat 16° C. The cells were spun down and the cell pellet was resuspend in5 mL of buffer. The resuspension was sonicated for 2 min on ice.

Bioassay: Fall army worm (FAW), corn ear worm (CEW), European corn borer(ECB) southwestern corn borer (SWCB) and diamond backed moth (DBM or Px)eggs were purchased from a commercial insectary (Benzon Research Inc.,Carlisle, Pa.). The FAW, CEW, ECB and BCW eggs were incubated to thepoint that eclosion would occur within 12 hrs of the assay setup. SWCBand DBM were introduced to the assay as neonate larvae. Assays werecarried out in 24-well trays containing multispecies lepidopteran diet(Southland Products Inc., Lake Village, Ark.). Samples of the sonicatedlysate were applied to the surface of the diet (diet overlay) andallowed to evaporate and soak into the diet. For CEW, FAW, BCW, ECB andSWCB, a 125 μl of sonicated lysate was added to the diet surface anddried. For DBM, 50 μl of a 1:2 dilution of sonicated lysate was added tothe diet surface. The bioassay plates were sealed with a plate sealingfilm vented with pin holes. The plates were incubated at 26° C. at 65%relative humidity (RH) on a 16:8 day:night cycle in a Percival for 5days. The assays were assessed for level of mortality, growth inhibitionand feeding inhibition.

For the western corn rootworm bioassay, the protein construct/lysate wasevaluated in an insect bioassay by dispensing 60 μl volume on the topsurface of diet in well/s of 24-well plate (Cellstar, 24-well, GreinerBio One) and allowed to dry. Each well contained 500 μl diet (Marrone etal., 1985). Fifteen to twenty neonate larvae were introduced in eachwell using a fine tip paint brush and the plate was covered withmembrane (Viewseal, Greiner Bio One). The bioassay was stored at ambienttemperature and scored for mortality, and/or growth/feeding inhibitionat day 4.

For Colorado Potato Beetle (CPB) a cork bore size No. 8 leaf disk wasexcised from potato leaf and was dipped in the protein construct/lysateuntil thoroughly wet and placed on top of filter disk (Millipore, glassfiber filter, 13 mm). 60 μl dH2O was added to each filter disk andplaced in each well of 24-well plate (Cellstar, 24-well, Greiner BioOne). The leaf disk was allowed to dry and five to seven first instarlarvae were introduced in each well using a fine tip paint brush. Theplate was covered with membrane (Viewseal, Greiner Bio One) and smallhole was punctured in each well of the membrane. The construct wasevaluated with four replicates, and scored for mortality and leaf damageon day 3.

Table 3 provides a summary of pesticidal activity against coleopteranand lepidoptera of the various sequences. Table code: “−” indicates noactivity seen; “+” indicates pesticidal activity; “NT” indicates nottested; “S” indicates stunt; “SS” indicates slight stunt; “LF” indicateslow feeding, “M” indicates mortality.

TABLE 3 Summary of Pesticidal Activity against Coleopteran andLepidoptera. WCR Mortality APG Seq ID FAW CEW BCW ECB SWCB CPB Px (%)APG00056 1 − − − − − − − 80-100% mortality APG00073 11 S − − − − NT −80-100% mortality APG00105 18 − − − − − NT − NT APG00121 29 − − − − − NT− 0-50% mortality APG00131 30 − − − − − NT − NT APG00152 36 M, S − − − −− NT 50-80% mortality APG00164 41 − − − − − + NT 0-50% mortalityAPG00175 49 SS − − − − NT + NT APG00176 50 − − − − − + + 80-100%mortality APG00342 142 − − − − − − − 80-100% mortality APG00388 159 NTNT NT NT NT − + NT APG00799 159 SS SS − − − NT − NT APG00801 160 − − − −− − − 0-50% mortality APG00422 177 − − − − M, SS + + NT APG00456 180 − −− − − NT − 0-50% mortality APG00462 183 NT NT NT NT NT + + NT APG00084229 − SS − NT NT NT NT 0-50% mortality APG00108 230 − − − − − NT −60-100% mortality APG00116 233 HM, S − − − SS NT − 80-100% mortalityAPG00134 240 − − − − − NT − 60-100% mortality APG00168 247 NT NT NT NTNT − + NT APG00177 248 M, S NT NT NT S NT − 60-100% mortality APG00186249 − − − NT − 0-50% mortality APG00194 251 SS − − − − NT − 80-100%mortality APG00200 254 NT NT NT NT NT + + NT APG00210 258 M, S − − − −NT NT 80-100% mortality APG00227 267 − − − − − NT NT 0-50% mortalityAPG00239 277 M, S − − − − NT NT 80-100% mortality APG00248 282 NT NT NTNT NT + NT NT APG00267 293 − − − − − − NT 50-80% mortality APG00273 296− − − − SS NT − NT APG00290 307 M, S − − − HM, S NT NT 80-100% mortalityAPG00291 308 M, S − − − − NT NT 80-100% mortality APG00297 313 M, S − −− − NT NT 50-80% mortality APG00500 357 − − − − − − + 60-100% mortalityAPG00647 372 − − − − M, SS NT − NT APG00698 374 − − − − M, SS NT + NT

Example 4. Pesticidal Activity Against Hemipteran

Protein Expression: Each of the sequences set forth in Table 4 wasexpressed in E. coli as described in Example 2. 400 mL of LB wasinoculated and grown to an OD600 of 0.6. The culture was induced with0.25 mM IPTG overnight at 16° C. The cells were spun down and the cellpellet was re-suspend in 5 mL of buffer. The resuspension was sonicatedfor 2 min on ice.

Second instar SGSB were obtained from a commercial insectary (BenzonResearch Inc., Carlisle, Pa.). A 50% v/v ratio of sonicated lysatesample to 20% sucrose was employed in the bioassay. Stretched parafilmwas used as a feeding membrane to expose the SGSB to the diet/samplemixture. The plates were incubated at 25° C.:21° C., 16:8 day:nightcycle at 65% RH for 5 days.

Mortality was scored for each sample. The results are set forth in Table4. A dashed line indicates no mortality was detected. The proteinslisted in table 4 showed 25% mortality or 50% mortality (as indicated)against southern green stinkbug (1 stinkbug out of 4 died). The negativecontrols (empty vector expressed binding domain and buffer only) bothshowed no mortality (0 stinkbugs out of 4).

TABLE 4 Summary of Pesticidal Activity against Hemipteran APG Seq IDTested against SGSB APG00173 47 25% APG00190 62 25% APG00388 159 25%APG00801 160 50% APG00196 252 50% APG00273 296 50% APG00291 308 25%APG00297 313 25%

Example 5. Transformation of Soybean

DNA constructs comprising each of SEQ ID NOs: 1-398 or active variantsor fragments thereof operably linked to a promoter active in a plant arecloned into transformation vectors and introduced into Agrobacterium asdescribed in PCT Application PCT/US2015/066702, filed Dec. 18, 2015,herein incorporated by reference in its entirety.

Four days prior to inoculation, several loops of Agrobacterium arestreaked to a fresh plate of YEP* medium supplemented with theappropriate antibiotics** (spectinomycin, chloramphenicol andkanamycin). Bacteria are grown for two days in the dark at 28° C. Aftertwo days, several loops of bacteria are transferred to 3 ml of YEPliquid medium with antibiotics in a 125 ml Erlenmeyer flask. Flasks areplaced on a rotary shaker at 250 RPM at 28° C. overnight. One day beforeinoculation, 2-3 ml of the overnight culture were transferred to 125 mlof YEP with antibiotics in a 500 ml Erlenmeyer flask. Flasks are placedon a rotary shaker at 250 RPM at 28° C. overnight.

Prior to inoculation, the OD of the bacterial culture is checked at OD620. An OD of 0.8-1.0 indicates that the culture is in log phase. Theculture is centrifuged at 4000 RPM for 10 minutes in Oakridge tubes. Thesupernatant is discarded and the pellet is resuspended in a volume ofSoybean Infection Medium (SI) to achieve the desired OD. The culturesare held with periodic mixing until needed for inoculation.

Two or three days prior to inoculation, soybean seeds are surfacesterilized using chlorine gas. In a fume hood, a petri dish with seedsis placed in a bell jar with the lid off. 1.75 ml of 12 N HCl is slowlyadded to 100 ml of bleach in a 250 ml Erlenmeyer flask inside the belljar. The lid is immediately placed on top of the bell jar. Seeds areallowed to sterilize for 14-16 hours (overnight). The top is removedfrom the bell jar and the lid of the petri dish is replaced. The petridish with the surface sterilized is then opened in a laminar flow foraround 30 minutes to disperse any remaining chlorine gas.

Seeds are imbibed with either sterile DI water or soybean infectionmedium (SI) for 1-2 days. Twenty to 30 seeds are covered with liquid ina 100×25 mm petri dish and incubated in the dark at 24° C. Afterimbibition, non-germinating seeds are discarded.

Cotyledonary explants are processed on a sterile paper plate withsterile filter paper dampened using SI medium employing the methods ofU.S. Pat. No. 7,473,822, herein incorporated by reference.

Typically, 16-20 cotyledons are inoculated per treatment. The SI mediumused for holding the explants is discarded and replaced with 25 ml ofAgrobacterium culture (OD 620=0.8-20). After all explants are submerged,the inoculation is carried out for 30 minutes with periodic swirling ofthe dish. After 30 minutes, the Agrobacterium culture is removed.

Co-cultivation plates are prepared by overlaying one piece of sterilepaper onto Soybean Co-cultivation Medium (SCC). Without blotting, theinoculated cotyledons are cultured adaxial side down on the filterpaper. Around 20 explants can be cultured on each plate. The plates aresealed with Parafilm and cultured at 24° C. and around 120 μmoles m-2s-1(in a Percival incubator) for 4-5 days.

After co-cultivation, the cotyledons are washed 3 times in 25 ml ofSoybean Wash Medium with 200 mg/l of cefotaxime and timentin. Thecotyledons are blotted on sterile filter paper and then transferred toSoybean Shoot Induction Medium (SSI). The nodal end of the explant isdepressed slightly into the medium with distal end kept above thesurface at about 45 deg. No more than 10 explants are cultured on eachplate. The plates are wrapped with Micropore tape and cultured in thePercival at 24° C. and around 120 μmoles m-2s-1.

The explants are transferred to fresh SSI medium after 14 days. Emergingshoots from the shoot apex and cotyledonary node are discarded. Shootinduction is continued for another 14 days under the same conditions.

After 4 weeks of shoot induction, the cotyledon is separated from thenodal end and a parallel cut is made underneath the area of shootinduction (shoot pad). The area of the parallel cut is placed on SoybeanShoot Elongation Medium (SSE) and the explants cultured in the Percivalat 24° C. and around 120 μmoles m-2s-1. This step is repeated every twoweeks for up to 8 weeks as long as shoots continue to elongate.

When shoots reach a length of 2-3 cm, they are transferred to SoybeanRooting Medium (SR) in a Plantcon vessel and incubated under the sameconditions for 2 weeks or until roots reach a length of around 3-4 cm.After this, plants are transferred to soil.

Note, all media mentioned for soybean transformation are found in Paz etal. (2010) Agrobacterium-mediated transformation of soybean and recoveryof transgenic soybean plants; Plant Transformation Facility of IowaState University, which is herein incorporated by reference in itsentirety. (See, agron-www.agron.iastate.edu/ptf/protocol/Soybean.pdf.)

Example 6. Transformation of Maize

Maize ears are best collected 8-12 days after pollination. Embryos areisolated from the ears, and those embryos 0.8-1.5 mm in size arepreferred for use in transformation. Embryos are plated scutellumside-up on a suitable incubation media, such as DN62A5S media (3.98 g/LN6 Salts; 1 mL/L (of 1000× Stock) N6 Vitamins; 800 mg/L L-Asparagine;100 mg/L Myo-inositol; 1.4 g/L L-Proline; 100 mg/L Casamino acids; 50g/L sucrose; 1 mL/L (of 1 mg/mL Stock) 2,4-D). However, media and saltsother than DN62A5S are suitable and are known in the art. Embryos areincubated overnight at 25° C. in the dark. However, it is not necessaryper se to incubate the embryos overnight.

The resulting explants are transferred to mesh squares (30-40 perplate), transferred onto osmotic media for about 30-45 minutes, and thentransferred to a beaming plate (see, for example, PCT Publication No.WO/0138514 and U.S. Pat. No. 5,240,842). DNA constructs designed toexpress the GRG proteins of the present invention in plant cells areaccelerated into plant tissue using an aerosol beam accelerator, usingconditions essentially as described in PCT Publication No. WO/0138514.After beaming, embryos are incubated for about 30 min on osmotic media,and placed onto incubation media overnight at 25° C. in the dark. Toavoid unduly damaging beamed explants, they are incubated for at least24 hours prior to transfer to recovery media. Embryos are then spreadonto recovery period media, for about 5 days, 25° C. in the dark, andthen transferred to a selection media. Explants are incubated inselection media for up to eight weeks, depending on the nature andcharacteristics of the particular selection utilized. After theselection period, the resulting callus is transferred to embryomaturation media, until the formation of mature somatic embryos isobserved. The resulting mature somatic embryos are then placed under lowlight, and the process of regeneration is initiated by methods known inthe art. The resulting shoots are allowed to root on rooting media, andthe resulting plants are transferred to nursery pots and propagated astransgenic plants.

Example 7. Pesticidal Activity Against Nematodes Heterodera Glycine's(Soybean Cyst Nematode) In Vitro Assay

Soybean Cyst Nematodes are dispensed into a 96 well assay plate with atotal volume of 100 uls and 100 J2 per well. The protein of interest asset forth in any one of SEQ ID NOs: 1-398 is dispensed into the wellsand held at room temperature for assessment. Finally, the 96 well platecontaining the SCN J2 is analyzed for motility. Data is reported as %inhibition as compared to the controls. Hits are defined as greater orequal to 70% inhibition.

Heterodera Glycine's (Soybean Cyst Nematode) on Plant Assay

Soybean plants expressing one or more of SEQ ID NOs: 1-398 are generatedas described elsewhere herein. A 3-week-old soybean cutting isinoculated with 5000 SCN eggs per plant. This infection is held for 70days and then harvested for counting of SCN cyst that has developed onthe plant. Data is reported as % inhibition as compared to the controls.Hits are defined as greater or equal to 90% inhibition.

Meloidogyne incognita (Root-Knot Nematode) In Vitro Assay

Root-Knot Nematodes are dispensed into a 96 well assay plate with atotal volume of 100 uls and 100 J2 per well. The protein of interestcomprising any one of SEQ ID NOs: 1-398 is dispensed into the wells andheld at room temperature for assessment. Finally the 96 well platecontaining the RKN J2 is analyzed for motility. Data is reported as %inhibition as compared to the controls. Hits are defined as greater orequal to 70% inhibition.

Meloidogyne incognita (Root-Knot Nematode) on Plant Assay

Soybean plants expressing one or more of SEQ ID NOs: 1-398 are generatedas described elsewhere herein. A 3-week-old soybean is inoculated with5000 RKN eggs per plant. This infection is held for 70 days and thenharvested for counting of RKN eggs that have developed in the plant.Data is reported as % inhibition as compared to the controls. Hits aredefined as greater or equal to 90% inhibition.

Example 8. Additional Assays for Pesticidal Activity

The various polypeptides set forth in SEQ ID NOs: 1-398 can be tested toact as a pesticide upon a pest in a number of ways. One such method isto perform a feeding assay. In such a feeding assay, one exposes thepest to a sample containing either compounds to be tested or controlsamples. Often this is performed by placing the material to be tested,or a suitable dilution of such material, onto a material that the pestwill ingest, such as an artificial diet. The material to be tested maybe composed of a liquid, solid, or slurry. The material to be tested maybe placed upon the surface and then allowed to dry. Alternatively, thematerial to be tested may be mixed with a molten artificial diet, andthen dispensed into the assay chamber. The assay chamber may be, forexample, a cup, a dish, or a well of a microtiter plate.

Assays for sucking pests (for example aphids) may involve separating thetest material from the insect by a partition, ideally a portion that canbe pierced by the sucking mouth parts of the sucking insect, to allowingestion of the test material. Often the test material is mixed with afeeding stimulant, such as sucrose, to promote ingestion of the testcompound.

Other types of assays can include microinjection of the test materialinto the mouth, or gut of the pest, as well as development of transgenicplants, followed by test of the ability of the pest to feed upon thetransgenic plant. Plant testing may involve isolation of the plant partsnormally consumed, for example, small cages attached to a leaf, orisolation of entire plants in cages containing insects.

Other methods and approaches to assay pests are known in the art, andcan be found, for example in Robertson and Preisler, eds. (1992)Pesticide bioassays with arthropods, CRC, Boca Raton, Fla.Alternatively, assays are commonly described in the journals ArthropodManagement Tests and Journal of Economic Entomology or by discussionwith members of the Entomological Society of America (ESA). Any one ofSEQ ID NOS: 1-398 can be expressed and employed in an assay as set forthin Examples 3 and 4, herein.

All publications and patent applications mentioned in the specificationare indicative of the level of skill of those skilled in the art towhich this invention pertains. All publications and patent applicationsare herein incorporated by reference to the same extent as if eachindividual publication or patent application was specifically andindividually indicated to be incorporated by reference.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity ofunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the appended claims.

That which is claimed is:
 1. A recombinant polypeptide having pesticidalactivity, comprising (a) a polypeptide comprising an amino acid sequencehaving at least 90% sequence identity to an amino acid sequence selectedfrom the group consisting of sequences set forth in SEQ ID NOs: 177,307, 372, 374, 396, 1, 11, 18, 29, 30, 36, 41, 49, 50, 142, 159, 160,180, 183, 229, 230, 233, 240, 247, 248, 249, 251, 254, 258, 267, 277,282, 293, 296, 308, 313, 357, 47, 62, 159, 160, 252, 296, 308, or 313;or, (b) a polypeptide comprising the amino acid sequence set forth inSEQ ID NOs: 177, 307, 372, 374, 396, 1, 11, 18, 29, 30, 36, 41, 49, 50,142, 159, 160, 180, 183, 229, 230, 233, 240, 247, 248, 249, 251, 254,258, 267, 277, 282, 293, 296, 308, 313, 357, 47, 62, 159, 160, 252, 296,308, or
 313. 2. The polypeptide of claim 1, further comprising aheterologous amino acid sequence.
 3. A composition comprising thepolypeptide of claim
 1. 4. A recombinant nucleic acid molecule encodingan amino acid sequence comprising (a) at least 90% sequence identity toan amino acid sequence selected from the group consisting of sequencesset forth in SEQ ID Nos: 177, 307, 372, 374, 396, 1, 11, 18, 29, 30, 36,41, 49, 50, 142, 159, 160, 180, 183, 229, 230, 233, 240, 247, 248, 249,251, 254, 258, 267, 277, 282, 293, 296, 308, 313, 357, 47, 62, 159, 160,252, 296, 308, or 313; or (b) the amino acid sequence set forth in SEQID NOs: 177, 307, 372, 374, 396, 1, 11, 18, 29, 30, 36, 41, 49, 50, 142,159, 160, 180, 183, 229, 230, 233, 240, 247, 248, 249, 251, 254, 258,267, 277, 282, 293, 296, 308, 313, 357, 47, 62, 159, 160, 252, 296, 308,or 313; wherein said recombinant nucleic acid molecule is not anaturally occurring sequence encoding said polypeptide.
 5. Therecombinant nucleic acid of claim 4, wherein said nucleic acid moleculeis a synthetic sequence designed for expression in a plant.
 6. Therecombinant nucleic acid molecule of claim 4, wherein said nucleic acidmolecule is operably linked to a promoter capable of directingexpression in a plant cell.
 7. The recombinant nucleic acid molecule ofclaim 4, wherein said nucleic acid molecule is operably linked to apromoter capable of directing expression in a bacteria.
 8. A host cellcomprising the recombinant nucleic acid molecule of claim
 4. 9. The hostcell of claim 8, wherein said host cell is a bacterial host cell.
 10. ADNA construct comprising a promoter that drives expression in a plantcell operably linked to a recombinant nucleic acid molecule comprising anucleotide sequence that encodes a polypeptide comprising an amino acidsequence having at least 90% sequence identity to an amino acid sequenceselected from the group consisting of sequences set forth in SEQ ID NOs:177, 307, 372, 374, 396, 1, 11, 18, 29, 30, 36, 41, 49, 50, 142, 159,160, 180, 183, 229, 230, 233, 240, 247, 248, 249, 251, 254, 258, 267,277, 282, 293, 296, 308, 313, 357, 47, 62, 159, 160, 252, 296, 308, or313.
 11. The DNA construct of claim 10, wherein said nucleotide sequenceis a synthetic DNA sequence designed for expression in a plant.
 12. Avector comprising the DNA construct of claim
 10. 13. A host cellcomprising the vector of claim
 12. 14. A composition comprising the hostcell of claim
 13. 15. The composition of claim 14, wherein saidcomposition is selected from the group consisting of a powder, dust,pellet, granule, spray, emulsion, colloid, and solution.
 16. Thecomposition of claim 15, wherein said composition comprises from about1% to about 99% by weight of said polypeptide.
 17. A method forcontrolling a pest population comprising contacting said pest populationwith a pesticidal-effective amount of the composition of claim
 3. 18. Amethod for producing a polypeptide with pesticidal activity comprisingculturing the host cell of claim 13 under conditions in which thenucleic acid molecule encoding the polypeptide is expressed.
 19. A planthaving stably incorporated into its genome a DNA construct comprising anucleotide sequence that encodes a protein having pesticidal activity,wherein said nucleotide sequence comprise (a) a nucleotide sequence thatencodes a polypeptide comprising the amino acid sequence of any one ofSEQ ID NOs: 177, 307, 372, 374, 396, 1, 11, 18, 29, 30, 36, 41, 49, 50,142, 159, 160, 180, 183, 229, 230, 233, 240, 247, 248, 249, 251, 254,258, 267, 277, 282, 293, 296, 308, 313, 357, 47, 62, 159, 160, 252, 296,308, or 313; or (b) a nucleotide sequence that encodes a polypeptidecomprising an amino acid sequence having at least 90% sequence identityto an amino acid sequence selected from the group consisting ofsequences set forth in SEQ ID NOs: 177, 307, 372, 374, 396, 1, 11, 18,29, 30, 36, 41, 49, 50, 142, 159, 160, 180, 183, 229, 230, 233, 240,247, 248, 249, 251, 254, 258, 267, 277, 282, 293, 296, 308, 313, 357,47, 62, 159, 160, 252, 296, 308, or
 313. 20. A transgenic seed of theplant of claim
 19. 21. A method for protecting a plant from an insectpest, comprising expressing in a plant or cell thereof a nucleotidesequence that encodes a pesticidal polypeptide, wherein said nucleotidesequence comprising (a) a nucleotide sequence that encodes a polypeptidecomprising the amino acid sequence of any one of SEQ ID NOs: 177, 307,372, 374, 396, 1, 11, 18, 29, 30, 36, 41, 49, 50, 142, 159, 160, 180,183, 229, 230, 233, 240, 247, 248, 249, 251, 254, 258, 267, 277, 282,293, 296, 308, 313, 357, 47, 62, 159, 160, 252, 296, 308, or 313; or (b)a nucleotide sequence that encodes a polypeptide comprising an aminoacid sequence having at least 90% sequence identity to an amino acidsequence selected from the group consisting of sequences set forth inSEQ ID NOs: 177, 307, 372, 374, 396, 1, 11, 18, 29, 30, 36, 41, 49, 50,142, 159, 160, 180, 183, 229, 230, 233, 240, 247, 248, 249, 251, 254,258, 267, 277, 282, 293, 296, 308, 313, 357, 47, 62, 159, 160, 252, 296,308, or
 313. 22. The method of claim 21, wherein said plant produces apesticidal polypeptide having pesticidal activity against at least oneof a lepidopteran pest, a coleopteran pest, or a hemipteran pest.
 23. Amethod for increasing yield in a plant comprising growing in a field aplant or seed thereof having stably incorporated into its genome a DNAconstruct comprising a promoter that drives expression in a plantoperably linked to a nucleotide sequence that encodes a pesticidalpolypeptide, wherein said nucleotide sequence comprises (a) a nucleotidesequence that encodes a polypeptide comprising the amino acid sequenceof any one of SEQ ID NOs: 177, 307, 372, 374, 396, 1, 11, 18, 29, 30,36, 41, 49, 50, 142, 159, 160, 180, 183, 229, 230, 233, 240, 247, 248,249, 251, 254, 258, 267, 277, 282, 293, 296, 308, 313, 357, 47, 62, 159,160, 252, 296, 308, or 313; or (b) a nucleotide sequence that encodes apolypeptide comprising an amino acid sequence having at least 90%sequence identity to an amino acid sequence selected from the groupconsisting of sequences set forth in SEQ ID NOs: 177, 307, 372, 374,396, 1, 11, 18, 29, 30, 36, 41, 49, 50, 142, 159, 160, 180, 183, 229,230, 233, 240, 247, 248, 249, 251, 254, 258, 267, 277, 282, 293, 296,308, 313, 357, 47, 62, 159, 160, 252, 296, 308, or 313.